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  • 1
    Electronic Resource
    Electronic Resource
    Transition of Thiobacillus ferrooxidans KG-4 from heterotrophic growth on glucose to autotrophic growth on ferrous-iron (1977)
    Springer
    Archives of microbiology 114 (1977), S. 193-195 
    Details
    ISSN: 1432-072X
    Keywords: Thiobacillus ferrooxidans ; Autotrophy ; Ferrous-iron oxidation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The reputedly obligately organotrophic Thiobacillus ferrooxidans KG-4 cultured on glucose contained a small proportion of cells which grew autotrophically on ferrous-iron.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00410784
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  • 2
    Electronic Resource
    Electronic Resource
    Kinetics of uranous ion and ferrous iron oxidation byThiobacillus ferrooxidans (1982)
    Springer
    Archives of microbiology 133 (1982), S. 33-37 
    Details
    ISSN: 1432-072X
    Keywords: Uranium oxidation ; Iron oxidation ; Oxygen uptake ; Carbon dioxide fixation ; Uranium toxicity ; Thorium toxicity ; Thiobacillus ferrooxidans
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Kinetic constants for the oxidation of uranous and ferrous ions byThiobacillus ferrooxidans were estimated. The kinetics indicate a direct biological mechanism for uranium oxidation. The complex interrelations of ferric, uranyl and uranous ion inhibition are considered.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00943766
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  • 3
    Electronic Resource
    Electronic Resource
    Studies on the growth of Thiobacillus ferrooxidans (1973)
    Springer
    Archives of microbiology 88 (1973), S. 285-298 
    Details
    ISSN: 1432-072X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary A method for enumeration of viable numbers of Thiobacillus ferrooxidans using membrane filters on ferrous-iron agar is presented. Factors affecting colony production were the concentration and brand of agar, pH of the medium, and type of membrane filter. The results suggest that inhibition of T. ferrooxidans by agar is a result of the acid hydrolysis of agar, the main product of which is d-galactose. Colony development was suppressed by aged medium, by acid-hydrolysed agar and by 0.1% galactose. Sartorius and Millipore membrane filters were suitable for the experiments, whereas Oxoid MF-50 membranes virtually suppressed the production of colonies. The method was employed to follow growth of T. ferrooxidans in pH 1.3 medium. The viable cell numbers were correlated with 14CO2-fixation and ferrous iron oxidation. Generation time was 6 h 22 min with a yield of 2.2×1012 organisms/g atom Fe2+ oxidized. Growth of T. neapolitanus on thiosulphate medium was not affected by agar-type or membrane filters and yield of the organism was 1.5×1013 organisms/g molecule Na2S2O3 oxidized.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00409941
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  • 4
    Electronic Resource
    Electronic Resource
    The uptake and assimilation of sulphate by Thiobacillus ferrooxidans (1975)
    Springer
    Archives of microbiology 105 (1975), S. 123-127 
    Details
    ISSN: 1432-072X
    Keywords: Thiobacillus ferrooxidans ; Sulphate Assimilation ; Sulphur Nucleotides
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Sulphate was rapidly bound by cell suspensions of Thiobacillus ferrooxidans. The binding was depressed by tetrathionate but was unaffected by Group VI anions, cysteine or methionine. Increasing uptake of sulphate was observed in cell suspensions incubated in the presence of ferrous iron. The bulk of 35S-sulphate was removed from the organisms by washing with dilute sulphuric acid and the remaining label was incorporated into cold trichloroacetic acid-soluble compounds. 35S-labelled adenosine 5′-sulphatophosphate was produced from ATP and 35S-sulphate by cell suspensions and in cell-free extracts. There was no evidence for the production of adenosine 3′-phosphate 5′-sulphatophosphate assayed by a very sensitive bioluminescence method.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00447125
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  • 5
    Electronic Resource
    Electronic Resource
    Studies on the growth ofThiobacillus ferrooxidans (1974)
    Springer
    Archives of microbiology 95 (1974), S. 153-164 
    Details
    ISSN: 1432-072X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Uranyl sulphate (0.2–0.9 mM) inhibited ferrous iron oxidation by growing cultures ofThiobacillus ferrooxidans. The addition of 5–100 mM uranium to the cultures caused immediate cessation of carbon dioxide fixation, rapid loss of viability and gradual depression of ferrous iron oxidation. Virtually no uranium was found in washed cells grown in the presence of subtoxic to toxic amounts of uranyl sulphate. Uranium-poisoned organisms appeared plasmolyzed in electron micrographs. Cultures tolerant to 5 mM UO2 2+ were develoepd by successive subculturing in increased uranium concentrations. The tolerance was maintained during subculturing in uranium-free medium. Frequency of mutants resistant to 1.0 and 1.5 mM UO2 2+ was 1 per 1.3×106 and 1 per 9.0×108, respectively. The frequency was increased in the presence of 15–150 mM nickel, zinc and manganese. In liquid cultures, bivalent cations and EDTA alleviated the toxicity of 2 mM uranyl sulphate.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02451757
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  • 6
    Electronic Resource
    Electronic Resource
    Studies on the growth ofThiobacillus ferrooxidans (1974)
    Springer
    Archives of microbiology 95 (1974), S. 165-180 
    Details
    ISSN: 1432-072X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Suspensions ofThiobacillus ferrooxidans in Warburg flasks oxidized ferrous iron and coupled14CO2 fixation to the oxidation. The sulphates of zinc, cobalt, copper, nickel or uranium (0.1–1.0 M) depressed the rate of Fe2+ oxidation: nickel and uranyl ions were the most inhibitory. Uranyl, copper and nickel ions inhibited iron-dependent CO2-fixation, the two former producing a marked uncoupling effect on CO2-fixation, similar to that produced by 2:4-dinitrophenol. Molybdate also inhibited iron oxidation. Incorporation of14C-labelled amino acids and glucose was largely dependent on energy from ferrous-iron oxidation and was also strongly inhibited by uranyl sulphate. Kinetic analysis of the inhibition of iron-oxidation by uranium indicated mixed competitive and non-competitive inhibition. Little binding of238U,63Ni or59Fe toT. ferrooxidans was observed and the effects of uranium were concluded to result mainly from loose binding at sites on the cell membrane concerned with iron-oxidation and possibly the transport of other metals. Molybdate probably interfered with sulphate-dependent steps of iron oxidation. Uncoupling of CO2-fixation probably resulted in part from interference with energy metabolism and could depend on transport of uranyl ions through the cell membrane. CO2-fixation by an uraniumtolerant culture (U+) was less sensitive to uranyl-inhibition than that by the wild-type strain (U−), but iron oxidation and CO2-fixation were much more sensitive to uranium when the U− organisms were previously cultured on thiosulphate rather than ferrous iron.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02451758
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  • 7
    Electronic Resource
    Electronic Resource
    Utilization of 35S-Thiosulphate and an appraisal of the role of ATP-sulphurylase in chemolithotrophic Thiobacillus ferrooxidans (1976)
    Springer
    Archives of microbiology 109 (1976), S. 205-208 
    Details
    ISSN: 1432-072X
    Keywords: Thiobacillus ferrooxidans ; Thiosulphate uptake ; Sulphate binding ; Sulphur metabolism ; ATP-sulphurylase
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Differentially labelled 35S-thiosulphate was taken up by washed cells of Thiobacillus ferrooxidans which were previously grown on thiosulphate. The uptake was proportional to the biomass over the range 0.5–4.0 mg dry wt. of bacteria and showed typical saturation kinetics with an estimated K m value of 0.5 mM for 35S-thiosulphate. Dithionate and Group VI anions inhibited the uptake, which was under pH control and had a temperature optimum of 50°C. In the absence of thiosulphate, the cells bound 35S-sulphate but the binding did not increase on prolonged incubation and the label could be removed completely by washing with dilute sulphuric acid. Increasing amounts of the label were incorporated from [outer-35S]thiosulphate into cellular materials over a 60-min period, whereas little or no assimilation was observed from either the [inner-35S]thiosulphate or 35S-sulphate. The kinetic properties of the sulphate-activating enzyme ATP_sulphurylase enriched from bacteria grown with either thiosulphate or ferrous-iron were similar although this enzyme has an assimilatory function only when the bacterium is grown with ferrous-iron.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00446629
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  • 8
    Electronic Resource
    Electronic Resource
    Accumulation and cellular distribution of uranium in Thiobacillus ferrooxidans (1983)
    Springer
    Archives of microbiology 135 (1983), S. 250-253 
    Details
    ISSN: 1432-072X
    Keywords: Uranium ; Thiobacilli ; Cell fractionation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The uptake and cellular distribution of UO 2 2+ were investigated in washed cell suspensions of Thiobacillus ferrooxidans. The uptake was dependent on external concentration of uranium (0.01–10.0 mM) and was influenced by the pH of the reaction mixture, but not by 1 mM transition metals ions or by the previous growth history of the cells. Cells inactivated by either ultraviolet radiation or potassium cyanide accumulated about 40% more uranium than did viable cells especially at a high, toxic UO 2 2+ concentration. Most of the uranium was associated with the cell wall and membrane fractions and relatively little uranium was detected in the cytoplasmic, lipopolysaccaride and periplasmic space material fractions. Cells poisoned with potassium cyanide were found to have an 8 to 11-fold increase in the cytoplasmic concentration of uranium.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00413476
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  • 9
    Electronic Resource
    Electronic Resource
    A novel method for the isolation of bacterial quinones and its application to appraise the ubiquinone composition of Thiobacillus ferrooxidans (1983)
    Springer
    Archives of microbiology 135 (1983), S. 77-80 
    Details
    ISSN: 1432-072X
    Keywords: Ubiquinone ; Isoprenoid quinone ; Quinone composition ; Thiobacillus ferrooxidans ; Mass spectrometry ; High performance liquid chromatography ; Thin layer chromatography
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A method was developed for the isolation of bacterial isoprenoid quinones. This method gave good yields and was superior to two standard methods that were also tested, because the cell membrane isolation and methanol extraction steps could be eliminated. The ubiquinone composition of Thiobacillus ferrooxidans was analyzed; only ubiquinone-8 was detected and its identification was resolved by thin layer chromatography, high performance liquid chromatography, and mass spectrometry.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00419487
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  • 10
    Electronic Resource
    Electronic Resource
    Proton translocation in intact cells of Thiobacillus denitrificans (1977)
    Springer
    Archives of microbiology 113 (1977), S. 11-16 
    Details
    ISSN: 1432-072X
    Keywords: Thiobacillus denitrificans ; Proton translocation ; Electron transport ; Oxygen uptake ; Fluorescence emission ; Throsulphate oxidation ; Membrane energization
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Proton translocation assessed by the quinacrine fluorescence technique was compared with oxygen uptake during thiosulphate oxidation by cells of Thiobacillus denitrificans. The addition of thiosulphate to cell suspensions resulted in an outwardly directed proton translocation as reflected by an increased quinacrine fluorescence. Compared to the O2 uptake activity, the proton translocating system was much more sensitive to proton conductors, other ionophores and inhibitors of electron transport. The results indicate that (a) the proton-translocation activity (membrane energization) is enhanced in aged cell suspensions, (b) intactness of the cytoplasmic membrane is essential for establishing a protonmotive force in cells, (c) the fluorescence increase and proton translocation are reversible processes, (d) inhibitors of electron transport may also act as proton conductors by altering the integrity of the cytoplasmic membrane.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00428573
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