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  • 1
    Electronic Resource
    Electronic Resource
    Chemical Characterization of Prickly Pear Seed Oil, Opuntia ficus-indica (1982)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of food science 47 (1982), S. 0 
    Details
    ISSN: 1750-3841
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Oil extracted from Opuntia ficus-indica seeds constituted 13.6% of the whole seed. Results of the physical and chemical analyses of the oil for refractive index, iodine number, saponification number, Reichert-Meissl number, Hehner value, acid value and unsaponifiable matter compared well with the characteristics of other commonly consumed vegetable oils. Thin-layer chromatography in conjunction with gas-liquid chromatography-mass spectrometry revealed a relatively high degree of unsaturation, 82%, with a linoleic acid content of 73.4% followed by palmitic, 12%, oleic 8.8% and stearic acid, 5.8%. Based on these results, Opuntia ficus-indica seed oil appears to be a good potential source of edible oil for human and/or animal consumption.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-2621.1982.tb12946.x
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  • 2
    Electronic Resource
    Electronic Resource
    Chemical Characterization and Edibility of the Oil Extracted from Citrullus colocynthis Seeds (1983)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of food science 48 (1983), S. 0 
    Details
    ISSN: 1750-3841
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Notes: The characteristics and composition of the crude oil extracted from Citrullus colocynthis seeds were examined. Data obtained for the iodine value, saponification number, Reichert-Meissl and others compare well with other edible oils. Thin-layer chromatography in conjunction with gas-liquid chromatography-mass spectrometry revealed over 75% degree of unsaturation major among which were linoleic, 50.6% and oleic acids, 25%. Upon storage, the oil showed relatively low peroxide values that were within the acceptable limits set for other vegetable oils. Animal experimentation trials on the oil using chicks as the experimental animals did not show any toxicity effects. Based on these results, Citrullus colocynthis oil might be of some potential use for animal feed and/or human consumption.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-2621.1983.tb14799.x
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  • 3
    Electronic Resource
    Electronic Resource
    Induction and suppression of RNase activity by growth regulators in senescing ragi leaves (1983)
    Oxford, UK : Blackwell Publishing Ltd
    Physiologia plantarum 58 (1983), S. 0 
    Details
    ISSN: 1399-3054
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Activity of RNase was studied in attached and detached leaves of 7-day-old ragi (Eleusine coracana Gaertn. cv PR 202) plants during senescence using crude enzyme extracts. The RNase activity was relatively constant in attached leaves. In excised leaves incubated in the dark there was a rapid rise in enzyme activity up to 48 h, followed by a decline. No such decrease was observed in the light. Benzimidazole and gibberellic acid suppressed the activity of RNase up to 48 h in the dark and 96 h in the light. Both the growth regulators also prevented the post-48 h decline in RNase activity of dark incubated excised leaves. Decline in the levels of chlorophyll and RNA in the illuminated excised leaves was not affected by 3-(3,4-dichlorophenyl)-1,1-dimethyIurea, but the inhibitor prevented the photo-induced rise in RNase activity. Cycloheximide and actinomycin D could completely prevent both detachment (increase in enzyme activity after the leaf is excised) and photo-induced rise in RNase activity. Benzimidazole and gibberellic acid prevented the rise in the activity of RNase on one hand and maintained it on the other by their influence on its biosynthesis. Photoinduction of RNase and photo-induced retardation of senescence are concluded to be two different processes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1399-3054.1983.tb05731.x
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  • 4
    Electronic Resource
    Electronic Resource
    Defining the locus of origin of a genetically determined electrophoretic variant of a multilocus enzyme system; the calcutta-1 of human LDH system is a B-locus variant (1981)
    Springer
    Human genetics 〈Berlin〉 57 (1981), S. 290-295 
    Details
    ISSN: 1432-1203
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Six (four Hindus, one Sikh, and one Muslim) out of 213 individuals originating from different parts of the Indian subcontinent (namely, Andhra Pradesh, Maharashtra, Uttar Pradesh, East Punjab, and West Punjab) were found to be Calcutta-1 (CAL1) variants of lactate dehydrogenase (LDH). The CAL1 variant was originally described (and thus, generally believed at present) as an allelic variant at the LDHA locus in chromosome 11. By using an improved Cellogel electrophoretic procedure the isozyme patterns observed in the erythrocytes and leukocytes of the variant have indicated that the CAL1 is not a variant of LDHA but that of LDHB, a chromosome 12 marker. This suggestion was supported by the isozyme patterns of LDH in a set of segregating clones of man-mouse somatic cell hybrids with the variant as human partner. Moreover, the variant cosegregated consistently with the human chromosome 12 and with the markers firmly assigned to the latter but not with human chromosome 11 or its markers in these hybrids. These results confirmed that the CAL1 is an LDHB variant.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00278947
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  • 5
    Electronic Resource
    Electronic Resource
    Basic defect in the expression of adenosine deaminase in ADA- SCID disease investigated through the cells of an obligate heterozygote (1981)
    Springer
    Human genetics 〈Berlin〉 56 (1981), S. 379-386 
    Details
    ISSN: 1432-1203
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The nature of the defect of a female baby who died of severe combined immunodeficiency (SCID) disease associated with adenosine deaminase deficiency (ADA-) was investigated. Since tissue or tissue culture material was not available for subsequent studies, the expression of ADA in her cells was investigated in the somatic cell hybrid clones derived from a fusion between the lymphocytes from one of her two obligate heterozygote parents and thymidine kinase deficient Chinese hamster (a3) fibroblasts. The results of analyses of the human chromosomes and biochemical markers in 12 independent clones and 27 subclones indicated that the ADA deficiency in the patient is determined probably by a mutation in the structural gene for ADA in chromosome 20 leading either to the production of catalytically defective molecules or to the cessation of the production of ADA. Incidentally, the involvement of chromosome 2, which carries a gene for adenosine deaminase complexing protein (ADCP), in the causation of ADA deficiency was excluded. The in vitro approach through the cells from an obligate heterozygote described in this paper may have a general application in pursuing studies on other cases of inborn errors of metabolism whenever the material from the affected individuals (i.e., the homozygotes) is not available or not suitable for direct investigations.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00274697
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  • 6
    Electronic Resource
    Electronic Resource
    Assignment of adenosine deaminase complexing protein (ADCP) gene(s) to human chromosome 2 in rodent-human somatic cell hybrids (1981)
    Springer
    Human genetics 〈Berlin〉 59 (1981), S. 317-323 
    Details
    ISSN: 1432-1203
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The experiments reported in this paper indicate that the expression of human adenosine deaminase complexing protein (ADCP) in the human-rodent somatic cell hybrids is influenced by the state of confluency of the cells and the background rodent genome. Thus, the complement of the L-cell derived A9 or B82 mouse parent apparently prevents the expression of human ADCP in the interspecific somatic cell hybrids. In the a3, E36, or RAG hybrids the human ADCP expression was not prevented by the rodent genome and was found to be proportional to the degree of confluency of the cell in the culture as in the case of primary human fibroblasts. An analysis of human chromosomes, chromosome specific enzyme markers, and ADCP in a panel of rodent-human somatic cell hybrids optimally maintained and harvested at full confluency has shown that the expression of human ADCP in the mouse (RAG)-human as well as in the hamster (E36 or a3)-human hybrids is determined by a gene(s) in human chromosome 2 and that neither chromosome 6 nor any other of the chromosomes of man carry any gene(s) involved in the formation of human ADCP at least in the Chinese hamster-human hybrids. A series of rodent-human hybrid clones exhibiting a mitotic separation of IDH1 and MDH1 indicated that ADCP is most probably situated between corresponding loci in human chromosome 2.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00295464
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  • 7
    Electronic Resource
    Electronic Resource
    Familial benign hypercalcaemia (FBH; McK. No. 14598, 1983): Linkage studies in a large Dutch family (1984)
    Springer
    Human genetics 〈Berlin〉 67 (1984), S. 452-454 
    Details
    ISSN: 1432-1203
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary By screening 27 hypercalcaemic and 21 normocalcaemic subjects in a large Dutch pedigree with familial benign hypercalcaemia (FBH; McK. No. 14598) (McKusick 1983) for more than 35 genetic markers, it was found that linkage of FBH can be excluded at about 25 centimorgans (cM) from GM, 20 cM from ABO, 15 cM from MNS and HLA, 10cM from JK and PI, and 5cM each from ACP1, AK1, ADA, GPT1, and PGP.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00291409
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  • 8
    Electronic Resource
    Electronic Resource
    Basic defect in the expression of adenosine deaminase in ADA -SCID disease (1983)
    Springer
    Human genetics 〈Berlin〉 63 (1983), S. 121-125 
    Details
    ISSN: 1432-1203
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary A specific competitive radioimmunoassay (RIA) was employed to quantify human adenosine deaminase molecules produced in human-Chinese hamster somatic cell hybrids. Studies on a set of hybrids in which the normal and aberrant expressions of adenosine deaminase (assigned earlier to human chromosome 20) were segregating, have demonstrated that in the patient with ADA -SCID disease reported by Herbschleb-Voogt et al (1981a), the deficiency of ADA activity was associated with a comparable deficiency of adenosine deaminase specific immuno-crossreacting material (ADA-CRM).
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00291530
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  • 9
    Electronic Resource
    Electronic Resource
    Mapping SB in relation to HLA and GL01 using cells from first-cousin marriage offspring (1983)
    Springer
    Immunogenetics 18 (1983), S. 503-512 
    Details
    ISSN: 1432-1211
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract A newly defined leukocyte antigen system, the secondary B-cell (SB) system, was shown to be linked to HLA. The SB marker has been investigated in lymphocyte donors presumed to be genetically homozygous for HLA-A through HLA-D/DR by virtue of descent from a first-cousin marriage and of phenotypic homozygosity for these HLA markers. Of 19 donors, 3 were found to be heterozygous for SB. Studies of the families of these three donors could not distinguish with certainty whether the heterozygosity resulted from SB/DR recombination or from “pseudohomozygosity” for HLA-A through -D/DR by inheritance of two genetically unrelated but similar haplotypes. However, our data favored the occurrence of SB/DR recombination with a meiotic distance perhaps as large as 3.3 cMorgan. Recombinations were identified which mapped SB between HLA-B and GL01. These studies demonstrate the usefulness of cells from first-cousin marriage offspring in mapping a polymorphic genetic system.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00364391
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  • 10
    Electronic Resource
    Electronic Resource
    Red cell glutathione peroxidase (GPX1) variation in Afro-Jamaican, Asiatic Indian, and Dutch populations (1984)
    Springer
    Human genetics 〈Berlin〉 66 (1984), S. 352-355 
    Details
    ISSN: 1432-1203
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary A Cellogel procedure for screening the electrophoretic variants of the human red cell glutathione peroxidase (GPX1) was described. Three hundred and ninety eight Dutch persons living in various parts of The Netherlands, 385 individuals born in various states of India, and 72 Jamaicans of African origin livig in Birmingham, UK, were screened for GPX1 variants. The Dutch were monomorphic, while one Afro-Jamaican female and two males and one female of the 116 Punjabis were found to be variants indistinguishable from each other in their pattern of electrophoresis. The clear five banded pattern of the variant indicated that the subunit structure of the human red cell glutathione peroxidase is most probably a tetramer and suggested that the variant is the expression of a heterozygote due to alleles at an autosomal locus. The corresponding phenotype was designated tentatively as GPX1 2-1 and the alleles as GPX1 *1 and GPX1 *2 respectively. The variant 2-1 was found to be identical to the “Thomas” variant described by Beutler and West (1974). Thus so far, in addition to the Afro-Americans and Ashkenazi Jews (Beutler et al. 1974), the Punjabis of the Indian subcontinent (this report) were found to exhibit the GPX1 polymorphism due to the GPX1 *2 allele. The data discussed in this paper (which included unpublished observations on several African and non-African populations) suggest that the GPX1 *2 allele is originally an African variant and hint that the present day Punjabis of Indian subcontinent, like Ashkenazi Jews, are “predominantly of Mediterranean origin with some proportion of African ancestry” (Mourant et al. 1976).
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00287640
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