ISSN:
1573-4935
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Chemistry and Pharmacology
Notes:
Abstract Marked differences were found in the electrophoretic profiles and amino-acid compositions of components prepared from rat glomerular basement membrane (GBM) by a number of different solubilization procedures. Treatment with reducing agent resulted in a simplified electrophoretic pattern which was characterized by the presence of a major collagenous component with a mol.wt, of 150 000. In contrast, detergent solubilized mainly lower-mol.-wt, material which had a more polar amino-acid composition. When both reagents were used together the majority of the basement-membrane material was soJubilized within 2 h and components with mol.wts, of 170 000 and 135 000 were predominant i n the pro-α region of the gel. Treatment for a further 16 h was required to solubilize higher-mol.-wt, material and to achieve maximum solubility of components in the pro-α region with mol.wts, of 185 000 and 150 000. These methods provide a means of separating subcomponents of rat GBM while avoiding the problems of degradation inherent in enzymatic procedures.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF01140495
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