ISSN:
1432-2307
Keywords:
Immunoelectron microscopy
;
Protein A-gold-technique
;
Biotin-avidin system
;
Amylase
;
Parotid gland
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Summary Specimens of the human parotid gland were studied by immuno-electron microscopy for the presence of amylase. Both the protein A-gold technique and the biotin-avidin-gold technique were used on the same specimens. Different fixations were tried. Amylase was detected in the zymogen granules in high amounts. This enzyme could even be seen in glutaraldehyde fixed and routinely embedded material. The subcellular localization of this enzyme opens a new field of functional morphological studies and studies in special tumours including acinic cell carcinomas.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00704063
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