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  • 1
    Electronic Resource
    Electronic Resource
    THE PARTICIPATION OF ENTERIC INHIBITORY NERVES IN ACCOMMODATION OF THE INTESTINE TO DISTENSION (1977)
    Oxford, UK : Blackwell Publishing Ltd
    Clinical and experimental pharmacology and physiology 4 (1977), S. 0 
    Details
    ISSN: 1440-1681
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: 1. The amounts by which various weights distended isolated segments from the distal colons of guinea-pigs were measured.2. Tetrodotoxin substantially reduced the distensibility of the intestine, but bretylium, guanethidine, hyoscine and methysergide had no significant effect.3. It is concluded that a local reflex involving enteric inhibitory nerves causes an accommodation of the intestine to stretch.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1440-1681.1977.tb02375.x
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  • 2
    Electronic Resource
    Electronic Resource
    Observations on the anatomy and amine histochemistry of the nerves and ganglia which supply the pelvic viscera and on the associated chromaffin tissue in the guinea-pig (1973)
    Springer
    Anatomy and embryology 140 (1973), S. 85-108 
    Details
    ISSN: 1432-0568
    Keywords: Autonomic nervous system ; Adrenergic nerves ; Chromaffin cells ; Pelvic viscera
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The organs of the lower abdominal and pelvic regions of the guinea-pig receive nerves from the inferior mesenteric ganglia and pelvic plexuses. The inferior mesenteric ganglia connect with the sympathetic chains, the superior mesenteric ganglia, the pelvic plexuses via the hypogastric nerves, and with the gut. Each pelvic plexus consists of anterior and posterior parts which send filaments to the internal generative organs and to the rectum, internal anal sphincter and other pelvic organs. The pelvic nerves enter the posterior plexuses, which also receive rami from the sacral sympathetic chains. The adrenergic neurons of the pelvic plexuses are monopolar, do not have dendrites and are supplied by few varicose adrenergic axons. Nearly all the nerves contain adrenergic fibres. After exposure to formaldehyde vapour the chromaffin cells appear brightly fluorescent with one or two long, often varicose, processes. Most of the chromaffin cells are in Zuckerkandl's organ or in chromaffin bodies associated with the inferior mesenteric ganglia. Groups of chromaffin cells are found along the hypogastric nerves and in the pelvic plexuses; they become smaller and fewer as regions more posterior to Zuckerkandl's organ are approached.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00520720
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  • 3
    Electronic Resource
    Electronic Resource
    The ramifications of adrenergic nerve terminals in the rectum, anal sphincter and anal accessory muscles of the guinea-pig (1973)
    Springer
    Anatomy and embryology 140 (1973), S. 109-128 
    Details
    ISSN: 1432-0568
    Keywords: Autonomic nervous system ; Gastrointestinal tract ; Adrenergic nerves ; Anal sphincter
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The anatomy and the adrenergic innervation of the rectum, internal anal sphincter and of accessory structures are described for the guinea-pig. The distribution of adrenergic nerves was examined using the fluorescence histochemical technique applied to both sections and whole mount preparations. The longitudinal and circular muscle of the rectum and the muscularis mucosae are all supplied by adrenergic nerve terminals. The density of the adrenergic innervation of the muscularis externa increases towards the anal sphincter. There is a very dense innervation of the internal anal sphincter, of the anal accessory muscles and of the corrugator ani. Non-fluorescent neurons in the ganglia of the myenteric plexus are supplied by adrenergic terminals. The ganglia become smaller and sparser towards the internal anal sphincter and non-ganglionated nerve strands containing adrenergic axons run from the plexus to the sphincter muscle. Adrenergic fibers innervate two interconnected ganglionated plexuses in the submucosa. Very few adrenergic nerve cells were found in the myenteric plexus and they were not found at all in the submucosa. The extrinsic arteries and veins of the pelvic region are heavily innervated by adrenergic nerves. Within the gut wall the arteries are densely innervated but there is little or no innervation of the veins.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00520721
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  • 4
    Electronic Resource
    Electronic Resource
    The origins of the adrenergic fibres which innervate the internal anal sphincter, the rectum, and other tissues of the pelvic region in the guinea-pig (1973)
    Springer
    Anatomy and embryology 140 (1973), S. 129-142 
    Details
    ISSN: 1432-0568
    Keywords: Autonomic nervous system ; Adrenergic nerves ; Pelvic viscera ; Gastrointestinal tract
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The adrenergic innervation of the pelvic viscera was examined by the fluorescence histochemical technique, applied to tissue from untreated guinea-pigs and from guinea-pigs in which nerve pathways had been interrupted at operation. It was found that adrenergic neurons in the inferior mesenteric ganglia give rise to axons which run in the colonic nerves and end in the myenteric and submucous plexuses and around the arteries of the distal colon. In the rectum, part of the innervation of the myenteric plexus and all of the innervation of the submucous plexus comes from the inferior mesenteric ganglia. The rest of the adrenergic innervation of the myenteric plexus comes from the posterior pelvic ganglia or the sacral sympathetic chains. The innervation of the blood vessels of the rectum is from the posterior pelvic ganglia. Adrenergic nerves run from the sacral sympathetic chains and pass via nerves accompanying the rectal arteries to the internal anal sphincter. Other adrenergic fibres to the internal anal sphincter either arise in, or pass through, the posterior pelvic plexuses. The anal accessory muscle is innervated by adrenergic axons arising in the posterior pelvic plexuses. Adrenergic nerves which run in the pudendal nerves, probably from the sacral sympathetic chains, innervate the erectile tissue of the penis.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00520326
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  • 5
    Electronic Resource
    Electronic Resource
    Immune lesions of noradrenergic neurones in rat central nervous system produced by antibodies to dopamine-β-hydroxylase (1977)
    [s.l.] : Nature Publishing Group
    Nature 267 (1977), S. 368-369 
    Details
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] Female Porton rats (200-250 g) were anaesthetised with intraperitoneal sodium pentobarbital (42 mg per kg body weight) and placed in a Kopf stereotaxic apparatus. A 21 or 23 gauge steel needle connected to a Harvard infusion pump was inserted stereotaxically in either a lateral or the third ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/267368a0
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  • 6
    Electronic Resource
    Electronic Resource
    Effect of cycloserine on histaminase (1975)
    Springer
    Inflammation research 5 (1975), S. 461-461 
    Details
    ISSN: 1420-908X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01972673
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  • 7
    Electronic Resource
    Electronic Resource
    In vivo anti-histaminic activity of histaminase (1975)
    Springer
    Inflammation research 5 (1975), S. 460-460 
    Details
    ISSN: 1420-908X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01972672
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  • 8
    Electronic Resource
    Electronic Resource
    The use of glycoxylic acid for the fluorescence histochemical demonstration of peripheral stores of noradrenaline and 5-hydroxytryptamine in whole mounts (1975)
    Springer
    Histochemistry and cell biology 41 (1975), S. 335-352 
    Details
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The reactions of glyoxylic acid with peripheral stores of noradrenaline and 5-hydroxytryptamine to provide a fluorescence histochemical method for their localization have been investigated. Incubation in glyoxylic acid, followed by drying and heating of whole mount preparations gives an intense and well localized reaction. For incubation, a concentration of 2% glyoxylic acid, buffered to pH 7 at room temperature for 30 minutes gives ideal results. The method is equally good if the pH is varied in the range 6 to 9 or if the tissue is stored in the incubation mixture for up to 6 hours. Ideal development of the fluorophore requires an initial excess of moisture in the tissue, that this moisture is driven off during development, and that the tissue is protected from further moistening. A suitable method of achieving these ends is to heat partially dried tissue at 100°C for 4 minutes and then cover it with paraffin oil. 5-hydroxytryptamine can be readily distinguished from noradrenaline because it forms a fluorophore after reaction at pH 3.5, whereas noradrenaline does not. Both amines can be visualized after incubation at neutral pH. Comparison with the formaldehyde vapour technique reveals three main advantages (and no disadvantages) of the glyoxylic acid method: (1) it gives a finer localization with higher fluorescence yield, (2) the glyoxylic acid method is less susceptible to variations in procedure and, (3) it is both simpler and quicker to apply.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00490076
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  • 9
    Electronic Resource
    Electronic Resource
    The presence of aromatic L-amino acid decarboxylase in certain intestinal nerve cells (1976)
    Springer
    Histochemistry and cell biology 48 (1976), S. 129-143 
    Details
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The presence of aromatic 1-amino acid decarboxylase (AADC) in nerve cell bodies of the intrinsic plexuses of the guinea-pig small intestine was demonstrated by incubating segments of intestine with 1-dopa in the presence of an inhibitor of monoamine oxidase, pargyline. After such incubation, some nerve cell bodies gave a fluorescence histochemical reaction indicative of the presence of a decarboxylated product of 1-dopa, probably dopamine. No fluorescence reaction occurred in the unincubated control or if the inhibitor of AADC, RO 4-4602, was included in the incubation mixture. The AADC-containing cell bodies apparently do not take up and store dopamine, because no fluorescence could be detected after incubation with dopamine and a monoamine oxidase inhibitor. The AADC-containing cells were found in about half of the ganglia of the submucous plexus of the guinea-pig small intestine, but were considerably less frequent in the myenteric plexus. They were also found in the other areas examined in this study, that is, in both enteric plexuses of the guinea-pig distal colon and of the small intestines of rabbits and rats.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00494551
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  • 10
    Electronic Resource
    Electronic Resource
    Aqueous aldehyde (Faglu) methods for the fluorescence histochemical localization of catecholamines and for ultrastructural studies of central nervous tissue (1978)
    Springer
    Histochemistry and cell biology 57 (1978), S. 285-295 
    Details
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Aqueous solutions combining a high concentration of formaldehyde (4%) with low concentrations of glutaraldehyde (0.5–1%) have been used to simultaneously localize amines by the formation of fluorescent products and to fix central nervous tissue for electron microscopy. The fluorescence reaction is produced by the aldehyde mixture at room temperature and the fluorescence is stable when the tissue is maintained in aqueous solution. This means that nerve cell bodies and terminal fields which contain catecholamines can be located accurately in vibratome sections at the light microscope level and, after further processing, can be examined under the electron microscope. With 1% glutaraldehyde in the aldehyde mixture, ultrastructural details are well preserved; there is no significant distortion of any component of the tissue. If vibratome or cryostat sections are dried against glass slides, the intensity of the fluorescence reaction is enhanced and the sections can be permanently mounted.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00492664
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