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  • 1975-1979  (7)
  • Biochemical taxonomy  (6)
  • Acetic Acid  (1)
  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Archives of microbiology 113 (1977), S. 139-141 
    ISSN: 1432-072X
    Keywords: Biochemical taxonomy ; Prototheca ; Chlorella ; Hydrogenase ; Acid tolerance ; Salt tolerance ; Sodium chloride ; Thermophily ; Gelatin liquefaction
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Five physiological and biochemical characters, which had proved to be valuable for the taxonomy of the genus Chlorella, were studied in the genus Prototheca. There is no hydrogenase activity and no liquefaction of gelatin. Most strains are very acidtolerant (limit of growth at pH 2.0 or 2.5) and very salt-tolerant (limit of growth at 4 or 5% NaCl). Two strains grow well at 38°C. The 16 strains, which were previously assigned to seven taxa, fall into four different groups. Our results tend to support the assumption that Prototheca might be related to Chlorella protothecoides.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Archives of microbiology 113 (1977), S. 143-144 
    ISSN: 1432-072X
    Keywords: Biochemical taxonomy ; Ankistrodesmus ; Scenedesmus ; Salt tolerance ; Sodium chloride ; Thermophily
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The genera Ankistrodesmus (including Raphidium) (17 strains) and Scenedesmus (27 strains) have a rather low salt tolerance without distinct speciesspecific differences. Only one strain of Ankistrodesmus and 23 strains of Scenedesmus are able to grow in the presence of 1% NaCl. Members of these genera therefore appear to be rather unsuitable for mass cultures in media based on sea water.-Two strains of Scenedesmus are thermophilic and grow well at 38°C.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    Archives of microbiology 119 (1978), S. 13-16 
    ISSN: 1432-072X
    Keywords: Biochemical taxonomy ; Chlorella ; Starch hydrolysis ; Amylase ; Extracellular enzymes
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Starch hydrolysis, i.e., the production of extracellular amylolytic enzymes, was found to be a specific character for most species of the genus Chlorella. C. fusca var. vacuolata, C. spec. 211-30, and C. spec. 211-11r hydrolyse starch, whereas C. vulgaris, C. fusca var. rubescens, C. zofingiensis, C. fusca var. fusca, C. minutissima, C. homosphaera, C. kessleri, C. luteoviridis, and C. protothecoides are unable to hydrolyse starch. Only C. sorokiniana and C. saccharophila appear heterogenous; within C. sorokiniana, 7 strains hydrolyse starch and 9 do not; and within C. saccharophila, 6 strains exhibit amylolytic activity and 2 do not. — A key for the identification, according to 9 easily determined physiological and biochemical characters, of the Chlorella species is presented.
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    Springer
    Archives of microbiology 116 (1978), S. 97-103 
    ISSN: 1432-072X
    Keywords: Biochemical taxonomy ; Chlorella ; DNA hybridization ; DNA homology
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract 1. DNA homology was studied in 12 Chlorella species. The DNA of 88 strains was hybridized with 3H-labelled DNA from C. fusca var. vacuolata 211-8b and from C. vulgaris 211-8m. The results indicate that the genus Chlorella is a heterogenous taxon which consists of several groups of species. 2. The “C. fusca group” comprises C. fusca var. vacuolata, C. fusca var. rubescens, and C. zofingiensis. Within this group, C. zofingiensis appears to be more closely related to C. fusca var. vacuolata than is C. fusca var. rubescens. C. fusca var. fusca does not belong to this group of taxa. 3. The “C. vulgaris group” consists of C. vulgaris, C. sorokiniana, and C. saccharophila. There are several strains which seem to assume a position intermediate between C. vulgaris and C. saccharophila. C. protothecoides is not related to this group of species. 4. Several groups of strains of C. sorokiniana with different base compositions (guanine + cytosine content) of their DNA appear to belong to the same taxon. 5. In addition to C. fusca var. fusca and C. protothecoides, also C. luteoviridis, C. minutissima, C. kessleri, and C. homosphaera seem to have so little relationship with the other species that their assignment to the genus Chlorella appears questionable.
    Type of Medium: Electronic Resource
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  • 5
    Electronic Resource
    Electronic Resource
    Springer
    Archives of microbiology 116 (1978), S. 105-107 
    ISSN: 1432-072X
    Keywords: Biochemical taxonomy ; Prototheca ; Chlorella ; Starch hydrolysis ; DNA ; Base composition ; GC content
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract 16 strains of the genus Prototheca do not produce extracellular amylolytic enzymes. The base composition of their DNA shows rather continuous values from 62% to 78% GC (guanine + cytosine). Their assignment to four species and their possible relationship with Chlorella protothecoides are discussed.
    Type of Medium: Electronic Resource
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  • 6
    Electronic Resource
    Electronic Resource
    Springer
    Archives of microbiology 103 (1975), S. 13-19 
    ISSN: 1432-072X
    Keywords: Biochemical Taxonomy ; Chlorella ; Fermentation Products ; Lactic Acid ; Acetic Acid ; Formic Acid ; Glycerol ; Ethanol ; Hydrogen
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The products of glucose fermentation were studied in 87 strains of the genus Chlorella. Lactic acid, acetic acid, formic acid, glycerol, ethanol, H2 and CO2 were identified. The lactic acid was shown to be d(-)lactic acid. The pattern of fermentation products is species-specific and can therefore be used as a taxonomic character. Lactic acid was found in C. fusca (varieties vacuolata, fusca, and rubescens), C. zofingiensis, C. vulgaris (var. vulgaris and f. tertia), and C. protothecoides. Formic acid and H2 appeared in those species which contain hydrogenase. Rather large amounts of glycerol were produced only by the most salt-tolerant species C. luteoviridis, C. saccharophila, and C. protothecoides.
    Type of Medium: Electronic Resource
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  • 7
    Electronic Resource
    Electronic Resource
    Springer
    Plant systematics and evolution 125 (1976), S. 129-138 
    ISSN: 1615-6110
    Keywords: Chlorophyceae ; Chlorella ; Biochemical taxonomy ; hydrogenase ; secondary carotenoids ; liquefaction of gelatin ; products of fermentation ; nitrate reduction ; thiamine requirement ; physiological tolerances ; thermophily ; DNA base composition
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract According to biochemical and physiological characters, 77 strains ofChlorella were assigned to 12 taxa. The characters used are presence or absence of hydrogenase, formation of secondary carotenoids under nitrogen-deficient conditions, liquefaction of gelatin, products of glucose fermentation, ability to reduce nitrate, thiamine requirement, acid tolerance, salt tolerance, thermophily, and base composition of DNA (GC content). On the other hand, certain nutritional characters, i. e., utilization of organic carbon and nitrogen compounds, were found to be more or less strain-specific, highly variable, and therefore unsuitable for taxonomy.
    Type of Medium: Electronic Resource
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