ISSN:
1573-5060
Keywords:
Oryza sativa
;
rice
;
gamma-radiation
;
temperature
;
mutation frequency
;
heading date
Source:
Springer Online Journal Archives 1860-2000
Topics:
Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
Notes:
Summary Dry seeds of rice, Oryza sativa L., containing about 13 percent moisture, were exposed to 60Co gamma raysat extremely low temperature (−196°C). M1 damage on survival rate, seedling height and seed set were found to become much smaller in the cooling treatment than in the not-cooled control. Chlorophyll and heading date mutation frequencies in the M2 generation were not affected by the decreased M1 damage caused by the cooling treatment, and the mutation frequencies could be significantly increased by applying higher doses combined with the cooling treatment. Frequency of sterile or semi-sterile mutants in the M2 generation was found to be significantly lower in the cooling treatment than in the not-cooled control. It is assumed from the experimental results that extremely low temperatures during irradiation could reduce the amount of physiological damage and gross chromosome aberrations. This results in an increased efficiency of producing minute deletions and/or point mutations.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00038936
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