ISSN:
1432-0428
Keywords:
Insulin receptor
;
cultured lymphocytes
;
insulin structure function relationship
;
insulin chains
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Summary The ability of insulin and the S-sulphonate A and B-chain derivatives to bind to a receptor on cultured human lymphocytes was evaluated. A receptor site for the S-sulphonate A-chain was identified and was strongly influenced by the intact insulin molecule. S-sulphonate A-chain weakly interfered with insulin binding. S-sulphonate B-chain showed no evidence of significant binding and did not interfere with insulin or S-sulphonate A chain binding.14CO2 production from14C-1-glucose was stimulated by insulin in cultured lymphocytes and this effect was blunted by S-sulphonate A-chain. The sulphhydryl blocking agent used in the production of insulin A-chain appears to be of critical importance.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF01219708
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