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  • 1975-1979  (5)
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Material
Years
Year
  • 1
    Electronic Resource
    Electronic Resource
    Functional Characterization in Vitro of Two Human T-cell Subpopuiations with Different Affinities to Sheep Erythrocytes (1979)
    Oxford, UK : Blackwell Publishing Ltd
    Scandinavian journal of immunology 9 (1979), S. 0 
    Details
    ISSN: 1365-3083
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: On the basis of their different abilities to form rosettes with unsensitized sheep erythrocytes (SRBC), two subpopuiations of human T lymphocytes were separated by the rosette sedi-mentation technique. In a series of in vitro experiments the T cells carrying high-avidity receptors for SRBC (‘active’ T cells) were shown to respond significantly better when stimulated with purified protein derivative (PPD) and allogeneic cells than the subpopulation carrying low-avidity reecplors ‘non-active’ T cells). On the other hand, the latter responded better to phytohaemaggiutinin, concanavalin A and pokcweed mitogen; it was likewise demonstrated that these cells were better stimulators in a mixed lymphocyte reaction. The role of monocytcs in mitogen and PPD responses was investigated by adding such cells to Fe-treated suspensions. These were found io be crucial for an optimal response of both subpopuiations. Furthermore, ditfereni patterns of activation were observed for the active and non-active cells. Especially interesting was the observation that the Con A response of the active fraction was more mono-cyte-dependent than that of the non-active one.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-3083.1979.tb03171.x
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Purification of Human Interferon by Antibody Affinity Chromatography, using Highly Absorbed Anti-interferon (1978)
    Oxford, UK : Blackwell Publishing Ltd
    Scandinavian journal of immunology 8 (1978), S. 0 
    Details
    ISSN: 1365-3083
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Antibodies against partially purified human leucocyte interferon (PIF) were bound to Sepharose 4B and crude interferons applied on this affinity column were purified, up to 8 × 105 interferon units (IFU) per mg protein in one step. Antibodies against PIF were absorbed with immobilized crude human leucocyte interferon bound to Sepharose, whereby antibodies against impurities were predominantly removed. Extensively absorbed antisera were coupled to Sepharose and used for antibody affinity chromatography of crude interferon preparations. Leucocyte and fibroblast interferons were purified in one step with around 100% recovery, up to 1 × 108 IFU per mg protein, and Namalva interferon up to 2 × 107 IFU/mg. SDS electrophoresis of affinity-purified leucocyte interferon revealed that the interferon activity appeared in two bands (19,000 and 23,000 D).
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-3083.1978.tb00538.x
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    Paper (German National Licenses)
    Fulltext
  • 3
    Electronic Resource
    Electronic Resource
    Human Leucocyte Interferon: Analysis of Effect on MLC and Effector Cell Generation (1979)
    Oxford, UK : Blackwell Publishing Ltd
    Scandinavian journal of immunology 9 (1979), S. 0 
    Details
    ISSN: 1365-3083
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Mixed lymphocyte cultures (MLC) showed decreased thymidine incorporation when interferon (IF) had been added to the culture medium. The cell-mediated lympholysis (CML) potential generated in mixed lymphocyte cultures grown in the presence of IF was substantially augmented at a certain concentration range. Highly purified leucocyte IF also had this effect, whereas mock preparations did not. The CML-augmenting property was found in the antiviral fraction after separation of semipurified leucocyte IF (by the method of Daht & Degree). The possibility that the effects were due lo a shift in kinetics after culturing in the presence of IF has been excluded. The killer-enhancing properties of IF did not seem to be due to an enhanced expression of histocompatibility antigens, the selective inhibition of CML suppressor cells, or a change in the specificity of recognized target cells. The results of secondary in vitro MLC-CML experiments, in which primary MLC had been carried out in the presence or absence of IF, indicated that the compound acts (in vitro) through preferential selection of cells that are less sensitive lo inhibition by IF and more likely to kill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-3083.1979.tb03280.x
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    Paper (German National Licenses)
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  • 4
    Electronic Resource
    Electronic Resource
    The actions of interferon are potentiated at elevated temperature (1978)
    [s.l.] : Nature Publishing Group
    Nature 274 (1978), S. 508-510 
    Details
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] Fig. 1 Enhancing effect of interferon on CML in MLR at different temperatures. Six MLR-CML temperature experiments are shown. Killer cells generated in MLR in the absence of interferon (-IF) and in the presence of 500 U ml"1 (+IF) are compared at 37 and 39 C. o, specific release by allogeneic ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/274508a0
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    Paper (German National Licenses)
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  • 5
    Electronic Resource
    Electronic Resource
    Modification of lymphocyte response to phytomitogens by polycations and polyanions (1978)
    Springer
    Cellular and molecular life sciences 34 (1978), S. 1224-1227 
    Details
    ISSN: 1420-9071
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The stimulation effect on mitogen-induced lymphocyte response by polycationic compounds such as polylysine, DEAE dextran protamine and methylated albumin, is studied at different serum-protein concentrations and mitogen concentrations. It is suggested that the polar interaction between polycationic compounds and glycoproteins are of major importance for reactivity of lymphocytes to mitogens.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01922972
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    Paper (German National Licenses)
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