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  • 1970-1974  (4)
  • 1
    Electronic Resource
    Electronic Resource
    Palo Alto, Calif. : Annual Reviews
    Annual Review of Genetics 4 (1970), S. 347-372 
    ISSN: 0066-4197
    Source: Annual Reviews Electronic Back Volume Collection 1932-2001ff
    Topics: Biology
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Palo Alto, Calif. : Annual Reviews
    Annual Review of Genetics 8 (1974), S. 15-50 
    ISSN: 0066-4197
    Source: Annual Reviews Electronic Back Volume Collection 1932-2001ff
    Topics: Biology
    Type of Medium: Electronic Resource
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  • 3
    ISSN: 1617-4623
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Revertants of an acu-6 mutant of Neurospora crassa have been isolated. One revertant, which showed temperature-sensitive growth on acetate (Fig. 2), was found to possess an abnormally thermolabile PEP carboxykinase (Fig. 3). The temperature-sensitive property mapped at, or extremely close to, the site of the original mutation, confirming that acu-6 is the structural gene for PEP carboxykinase. A group of acu-6 mutants were examined by polyacrylamide gel electrophoresis for the presence of a protein migrating in the same position as PEP carboxykinase. Two of the seven mutants examined were found to possess such protein and both of these show inter-allelic complementation. When grown on acetate the complementing heterokaryons showed about 5% of the wild type level of PEP carboxykinase activity. This activity was more thermolabile than that in wild type (Fig. 6) and the heterokaryons showed temperature-sensitive growth on acetate (Fig. 5).
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    Springer
    Molecular genetics and genomics 109 (1970), S. 309-322 
    ISSN: 1617-4623
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Fine structure map expansion is a marker effect which can be explained on the basis of the repair of mismatched bases in hybrid DNA. The chance of a mismatched base pair being corrected independently of a closely linked mismatched pair will sharply increase as the distance between the two sites becomes greater than the length of the DNA segment involved in the correction process. The consequences of this explanation are worked out and it is shown that, if it is true, the mapping curve should show three phases: an initial additive phase when the recombining sites are closely linked, a phase of increased slope—map expansion-and a final additive phase of reduced slope beyond the expansion region. Comparison of the initial and final slopes should yield information on the relation between gene conversion and crossing-over. Many sets of experimental data show a clear transition from the initial additive region to that of map expansion, but evidence for the predicted final phase is scanty, possibly because fine structure maps cover too small a region of the chromosome. Using data from genes with known products, estimates can be made of the minimum length of the DNA segments involved in correction. These are calculated as about 40 nucleotides in fission yeast and at least 130 nucleotides in Neurospora.
    Type of Medium: Electronic Resource
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