ISSN:
1432-1459
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Description / Table of Contents:
Zusammenfassung Die Fettsäuren der dünnschichtchromatographisch fraktionierten Liquorlipide von 7 Gesunden, 8 Patienten mit Multipler Sklerose und 2 Patienten mit myatrophischer Lateralsklerose wurden gaschromatographisch analysiert. Die Gruppe der MS-Patienten wurde unterteilt in solche, deren Liquor unmittelbar nach einem akuten Krankheitsschub und in solche mit mehr chronischem Verlauf, bei denen der Liquor im schubfreien Intervall entnommen worden war. Die Fettsäurenzusammensetzung der Liquorlipidfraktionen — Phosphatide, freie Fettsäuren, Triglyceride und Cholesterinester — von Normalen ist konstant. Auffallend ist der hohe Anteil von Myristinsäure von durchschnittlich 21,5%. Die pathologischen Liquores zeigen in der Gruppe der MS-Patienten im akuten Schub gegenüber den Vergleichsgruppen in der Triglycerid- und Cholesterinesterfraktion eine signifikante Vermehrung der zwei- und mehrfach ungesättigten Fettsäuren. Fragen des Zusammenhangs zwischen synchronen Veränderungen an den Lipiden und am Eiweiß der Cerebrospinalflüssigkeit werden unter dem Aspekt einer herabgesetzten Hirnliquorschranke bei entzündlichen Entmarkungskrankheiten diskutiert.
Notes:
Summary The fatty acid moiety of lipids of cerebrospinal fluid (CSF) of 7 normal persons, 8 patients with multiple sclerosis (MS), and 2 suffering from myatrophic lateral sclerosis were identified by gas-liquid chromatography (GLC) following the separation of CSF lipids by thin layer chromatography (TLC). The MS patients were assigned to two groups. Samples of the first group were obtained immediately after an exacerbation. From the second group — comprising cases with more chronical courses — CSF was obtained during remissions or intervals free of exacerbations. The fatty acid profile of CSF lipid fractions — phosphatides, free fatty acids, triglycerides and cholesterol ester — shows a constant pattern in normal subjects. The high proportion of C14-acids (myristic) with an average percentage of 21.5 is particularly striking. The constant occurrence of C24-acids (lignoceric) in all four fractions of normal CSF seems to indicate that normal CSF lipids are already altered breakdown products of cerebral lipids. Pathological samples of CSF from acute MS patients showed a significant increase in dienoic and polyenoic fatty acids of triglycerides and cholesterol ester as compared to the second group. It is pointed out that Neumann (1967) discovered in applying the potassium permanganate reduction test to 5300(!) samples of CSF that in 88% of the cases a decrease in reduction time is accompanied by an increase in total protein. Experiments by Tourtellotte (1969) showed concurrent changes in lipids and protein content of CSF in acute inflammatory disorders. It seems plausible to explain this by an impaired brain-CSF barrier. The augmentation of polyenoic fatty acids in CSF of MS patients with exacerbations — found in our investigations — is likely to be also related to an impaired brain-CSF barrier. Neumann's finding which is striking in several respects might be explained by the above results. In concluding attention should be called to the recently published monograph by H. Pilz in which a thorough account of the mutual interdependence of lipids and proteins of CSF may be found. This monograph on normal and pathological lipids of CSF also gives a detailed survey of methods and results of lipid studies of CSF.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00316666
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