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  • 1965-1969  (2)
  • Hämoglobin  (1)
  • Shunt-Diffusion of O2  (1)
  • 1
    Electronic Resource
    Electronic Resource
    The oxygen supply of the rat kidney: Measurements of intrarenalpO2 (1969)
    Springer
    Pflügers Archiv 309 (1969), S. 328-349 
    Details
    ISSN: 1432-2013
    Keywords: O2-Microelectrodes ; Measuring of LocalpO2 ; Shunt-Diffusion of O2 ; Critical O2-Supply Pressure ; Oxygen Tension in the Kidney ; Sauerstoff-Mikroelektroden ; LokalepO2-Messung ; O2-Diffusions-Shunt ; Kritischer Sauerstoffdruck ; Sauerstoffdrucke in der Niere
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Using a newly developed platinum-O2-microeletrode [30] based on the design ofSilver [37] the construction and properties of which are described,pO2-measurements in the parenchyma of the blood-perfused and the cell-free perfused rat kidney were carried out. By continuous recording of thepO2 during slow (150 μ×min−1) insertion of the O2-electrode into the respiring tissue two regions of distinctly different meanpO2-values were found. In the outer region which extends from the renal surface to a depth of about 3–4 mm (corresponding anatomically with the renal cortex) largepO2-differences exist close to each other. In the blood-perfused kidney the maximum corticalpO2-values lie in the range of arterialpO2 the lowest values at about 10 Torr. In the cortex of the cell-free perfused kidney the maximumpO2-values lie considerably below the arterialpO2. In both the blood perfused and in the cell-free perfused kidney at centripetal movement of the O2-electrode the cortical region of high and fluctuatingpO2 is followed by a narrow zone (≈200 μ radial extension) of a steep decrease of the meanpO2. At further insertion in both preparations thepO2 remains at lowpO2-values of ca. 10 Torr. Anatomically, this latter region of low and constantpO2 corresponds to renal medulla and pelvis. By recording the decrease of parenchymalpO2 after sudden stop of the perfusion attempts were made at measuring the critical local O2-supply pressure. In the cortex of the cell-free perfused kidney critical local O2-supply pressures between 6 and 28 Torr with a maximum abundance at 8 Torr were found. The qualitative and quantitative implications of the presented data on the conditions of parenchymal O2-supply are discussed. The results are interpreted as an indication for the arteriovenous shunt (bypass)-diffusion of considerable amounts of oxygen, especially under the conditions of the cell-free perfusion. Furthermore, it follows from the data presented that even at high venous O2-pressures and high meanpO2-values in the parenchyma regions of local anoxia may exist.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00587756
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  • 2
    Electronic Resource
    Electronic Resource
    Photometrische Messungen am schlagenden Herz von Myxine glutinosa L. zur Erfassung intracellulär gelegener Hämoproteine (1968)
    Springer
    Pflügers Archiv 299 (1968), S. 252-260 
    Details
    ISSN: 1432-2013
    Keywords: Myocardium ; Haemoglobin ; Myoglobin ; Cytochromes ; Cyclostomata ; Myokard ; Hämoglobin ; Myoglobin ; Cytochrome ; Cyclostomata
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Zusammenfassung 1. Spektralphotometrische Untersuchungen am Herzen des primitiven Hexenfisches Myxine glutinosa L. ergaben folgendes: In der Herzmuskulatur von Myxine existiert ein dem Hämoglobin sehr ähnliches Hämoproteid, dessen α-Bande (578.3 nm) gegenüber der des Hämoglobins (577,0 nm) um durchschnittlich 1,3 nm zu längeren Wellenlängen verschoben ist. Die Cytochrome a/a3 (603–609 nm), c (548,9 nm) und b (562,5 nm) sind in der Herzmuskulatur nachzuweisen. 2. Es wurde eine Perfusionsanordnung entwickelt, die die photometrische Messung am isolierten, spontan schlagenden, blutfrei perfundierten Herzen von Myxine ermöglicht. 3. Es wurde eine Methode der Filterpapierphotometrie entwickelt, die auf einfache Weise die Messung geringer Flüssigkeitsmengen (z. B. Blut) gestattet und mit der Cuvettenmethode identische Werte liefert.
    Notes: Summary 1. The heart of the primitive hagfish Myxine glutinosa L. has been directly examined spectrophotometrically. In the cardiac musculature there is a haemoprotein very similar to the haemoglobin. Compared with haemoglobin the α-band (578,3 nm) of this haemoprotein is shifted by 1,3 nm towards longer wavelengths. Cytochromes a+a3 (603–609 nm), cytochrome c (548,9 nm) and cytochrome b (562,5 nm) were also found in the cardiac muculature. 2. Using a special perfusion arrangement the isolated, erythrocyte-free, spontaneously beating hearts of Myxine were examined spectrophotometrically. 3. A spectrophotometric method employing filter paper as a sample holder has been developed. This allows measurements of reflection spectra on small quantities of liquids (e.g. blood). The results are identical with those using standard methods.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00362588
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
    Fulltext
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