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  • 1960-1964  (24)
  • 1930-1934
  • 1860-1869
  • Biochemistry and Biotechnology  (21)
  • Synthetic methods  (3)
  • 1
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 5 (1963), S. 41-48 
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Bacillus subtilis NRRL B-2612, isolated at this laboratory, was used to produce polyglutamic acid from devitalized wheat gluten. Conditions for production of the polypeptide were investigated. When NRRL B-2612 was grown on wheat gluten in shaken flasks with phsophate buffer and salts at pH 6.5, yields of polymer reflected a 25-40% incorporation of glutamic acid into polyglutamic acid. Repeated alcoholic precipitation yielded peptides containing between 45 and 90% D-glutamic acid. Physical and chemical properties of a typical purified polyglutamic acid indicated that the product was a gamma-linked polypeptide composed of 75% D-glutamic acid and 25% L-glutamic acid with a molecular weight of approximately 200,000.
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Biochemical and Microbiological Technology and Engineering 2 (1960), S. 205-214 
    ISSN: 0368-1467
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Oxygen tension in aqueous media of the order of 10-6 to 10-9 molar is measured amperometrically by means of a stationary inert metal electrode. The electrolytic cell is connected into a high resistance bridge circuit, which is energized from a low resistance d.c. source of voltage adjusted to give a potential difference across the cell corresponding to a position on the diffusion current plateau of the first oxygen wave. This cell potential is monitored by means of a vacuum tube millivoltmeter and maintained constant. A vacuum tube millivoltmeter measures the bridge output. The bridge is initially balanced with the medium deoxygenated; subsequent introduction of low concentrations of oxygen dissolved in the medium unbalance the bridge. Either the resulting bridge output voltage, as read on the millivoltmeter, or the resistance change in the bridge arm opposite the cell, is related to the oxygen tension by means of a previous calibration. Both quantities are directly proportional to oxygen concentration, at least over limited ranges of such concentration changes. The method is also applicable to the estimation of other substances which can be discharged under amperometric conditions at a stationary electrode.
    Additional Material: 6 Ill.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 5 (1963), S. 309-330 
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Biological purification of water pollutants depends upon a complex balance of organisms and environment conducive to effective metabolism. The bench scale test is useful to determine effects of a given waste water on that balance and to suggest controls promoting optimum purification. Applicability of laboratory results depends, in part, upon an experimental design similating anticipated operating conditions. This paper discusses advantages and limitations of historical bench-tests as a guide for design of tests giving data that are more useful during full-scale operation.
    Additional Material: 5 Tab.
    Type of Medium: Electronic Resource
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  • 4
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Two sources of oxygen for man in closed-cycle space system environment have been considered in previous studies: (1) photosynthesis using algae, and (2) electrolysis of water. The latter system appears to be the most promising from the standpoints of energy and weight requirements and ability to operate in a zero gravity field. The surplus hydrogen produced by the electrolysis of water may be utilized together with waste carbon dioxide, part of the oxygen, and waste urea by bacteria of the genus Hydrogenomonas to produce cellular protein which might be used as a source of food. A continuous culture system for the propagation of hydrogen-fixing bacteria consists of a baffled borosilicate glass culture vessel provided with an impeller, a reservoir vessel for the culture medium, and an overflow vessel for collecting the bacterial cells removed from the culture vessel. Complete feedback control of all parameters affecting growth can be provided by hydrogen, oxygen, and carbon dioxide sensors, and a pH electrode in the culture medium. In addition, total pressure is monitored. Cell density is controlled in the optimum range by means of a photoelectric cell which dictates the amount of fresh medium to be added and the amount of cells to be removed. Operating data indicate that some of the key parameters are the ratio of hydrogen, oxygen, and carbon dioxide in solution. The harvested bacterial substance is high in protein, which contains all the essential amino acids.
    Additional Material: 2 Ill.
    Type of Medium: Electronic Resource
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  • 5
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: The sandwich paper electrophoresis technique, such as that of Foster (1952b), was modified to permit routine examination of as many as 15 samples of materials such as bacterial cultures, simultaneously, and with greater rapidity than with paper chromatography techniques. By the use of borate buffer at pH 9.6 with aniline hydrogen oxalate for development, materials such as glucose, fructose, 2-ketogluconic acid, and 5-ketogluconic acid could be separated in three hours.
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
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  • 6
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: A strain of Torulopsis magnoliae, an osmophilic yeast isolated from flowers, produced an extracellular hydroxy fatty acid glycoside of sophorose which was easily recoverable as a heavier-than-water “oil”. The yield of this product was greatly increased and the nature of the hydroxy fatty acid portion of the molecule was modified, within limits, by the addition of fatty acid esters, hydrocarbons, or glycerides. Yields of hydroxy fatty acids of up to 90% of the substrate added were achieved. Commercially available substrates such as tall oil fatty acids, molasses, and corn steep liquor could be used. Good aeration was necessary, and control of the concentration of nitrogenous substrates and of temperature improved the yields.
    Additional Material: 7 Tab.
    Type of Medium: Electronic Resource
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  • 7
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 4 (1962), S. 281-297 
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Strains of the green algae Scenedesmus obliquus, Chlorella vulgaris, and C. pyrenoidosa 7-11-05, and of the blue-green algae Fremyella diplosiphon, Plectonema calothricoides, Phormidium luridum, and Synechococcus lividus have been mass cultured autotrophically in essentially pure D2O. Closed Lucite rocking-box units containing 5 liters of culture media can produce 1 to 2 g. (dry weight) per day of fully deuteriated algae or of fully deuteriated, fully 15N-substituted algae. Racks for six such culture units are readily constructed from commerically available parts. Operation has been routine and essentially trouble-free. The algae produced have proved to be an excellent source of completely isotopically substituted metabolites.
    Additional Material: 6 Ill.
    Type of Medium: Electronic Resource
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  • 8
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 4 (1962), S. 5-16 
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: A multi-stage pilot fermentor was designed which consists of nine stainless steel cylindrical compartments 2 ft. in length and 8 in. in diameter, joined end to end, giving a tube with a total length of 18 ft. The whole apparatus was mounted with its axis in a horizontal plane. Each compartment was sealed off from its neighboring compartment by a separatory plate having a 1-in. overflow hole in the upper half. Shafts 9½ feet long extended from each end of the fermentor through the compartments to the center. Agitation was provided by a multitude of stainless steel blades mounted at right angles to the shafts and spaced at regular intervals along the shafts. Stationary, stainless steel comb-shaped baffle plates were installed vertically at the bottom of each compartment to increase mixing efficiency. Constant leaving and re-entering of the fermentation liquor by the blades upon rotation imparts a shattering of the liquid surface thus preventing the accumulation of mycelial aggregates on the walls above the fermentation liquor. The mechanical performance of the described fermentor has proved excellent and several trial fermentations (antibiotic formation and steroid bioconversion) are described.
    Additional Material: 7 Ill.
    Type of Medium: Electronic Resource
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  • 9
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 4 (1962), S. 79-86 
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: An apparatus is described for the aeration of a series of tissue cultures at rates which may be varied independently for each vessel. The composition of the aerating gas can be adjusted to any desired percentages of CO2 and O2. While designed particularly for study of the aeration requirements of submerged tissue growth, the equipment is equally adaptable for study of submerged growth of bacterial and other systems. The apparatus has been designed for future scale-up and is adaptable to both the overlay and bubble-through types of aeration. Flow rates necessary for producing mixtures of 5.0% CO2 and 95.0% air or 5.6% CO2, 14.0% O2, and 80.4% nitrogen are given. The described system has been operated satisfactorily and has proved to be an inexpensive and useful research tool.
    Additional Material: 2 Ill.
    Type of Medium: Electronic Resource
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  • 10
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 5 (1963), S. 83-85 
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: The inclusion of ethionine in Streptomyces rimosus fermentation resulted in the production of N-methylethyloxytetracycline. The compound has been isolated, crystallized, and a number of its chemical properties determined.
    Additional Material: 1 Tab.
    Type of Medium: Electronic Resource
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