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  • 1960-1964  (2)
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  • 1
    Electronic Resource
    Electronic Resource
    An enzyme(s) from aStreptomyces sp. to prepare mould ‘protoplasts’ (1963)
    Springer
    Cellular and molecular life sciences 19 (1963), S. 82-83 
    Details
    ISSN: 1420-9071
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Résumé Les auteurs montrent l'action d'une préparation enzymique duStreptomyces GM sur la paroi cellulaire des champignons, donnant lieu à la formation de «protoplastes». De tous les champignons traités avec l'enzyme (strepzime),Fusarium culmorum semble s'être montré le plus sensible à cette digestion enzymatique.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02148030
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Formation of protoplasts ofFusarium culmorum by strepzyme (1964)
    Springer
    Antonie van Leeuwenhoek 30 (1964), S. 33-44 
    Details
    ISSN: 1572-9699
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Spherical protoplast-like structures can be liberated from hyphae ofFusarium culmorum by the action of an enzyme preparation obtained from the cell-free supernatant of the growth medium ofStreptomyces RA. In a previous publication this lytic system was named “strepzyme”. Treatment was carried out in a citrate phosphate buffer containing 0.8m mannitol or KCl as a stabilizing agent. If the concentration of the stabilizer was lowered to less than 0.2m the protoplasts lysed. These osmotically sensitive structures vary largely in size, are resistant to high speed centrifugation, and stable when kept in the cold. Protoplasts from other molds could also be obtained. The protoplasts could be observed to emerge through pores in the hyphal wall leaving behind delicate but rigid empty cell walls resistant to enzymic digestion and recognizable under the phase-contrast microscope. After sudden and complete lysis of protoplasts some membrane structures were observed. Attempts to obtain electron micrographs of the latter have failed. It was concluded that in the strain ofFusarium studied the strepzyme removes some constituent of the cell wall facilitating the extrusion of the protoplasts.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02046698
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
    Fulltext
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