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  • 1
    Electronic Resource
    Electronic Resource
    Methodische Untersuchungen zur polarographischen Messung der Atmung und des „kritischen Sauerstoffdrucks” bei Mitochondrien und isolierten Zellen mit der membranbedeckten Platinelektrode (1972)
    Springer
    Pflügers Archiv 337 (1972), S. 19-28 
    Details
    ISSN: 1432-2013
    Keywords: Mitochondrial Respiration Rate ; $$P_{{\text{O}}_{\text{2}} }$$ Electrode ; Critical Oxygen Tension
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The respiration of cells and mitochondria of different rat tissues and of ascites tumor cells was measured with a polarographic method. The rate of respiration (μl O2/mg mitochondria protein×min) was controlled by the substrate, by the addition of ADP and by the temperature (25° and 37°C). By means of a membrane-covered platinum electrode no correlation was found between the rate of respiration and the $$P_{{\text{O}}_{\text{2}} }$$ below of which the rate of oxygen consumption decreases. Under optimum conditions especially at saturating concentrations of ADP and substrate, the rate of respiration (measured between ∼120 and 0 Torr $$P_{{\text{O}}_{\text{2}} }$$ ) is not sufficiently constant to allow the definition of a “critical $$P_{{\text{O}}_{\text{2}} }$$ ” as the $$P_{{\text{O}}_{\text{2}} }$$ measured polarographically, at which the rate of respiration drops to 90% of the maximum rate. Due to the variations in the $$P_{{\text{O}}_{\text{2}} }$$ measurements the above “critical $$P_{{\text{O}}_{\text{2}} }$$ ” becomes strongly dependent on the method of computation used to fit the $$P_{{\text{O}}_{\text{2}} }$$ values measured to a straight line and to measure the rate of respiration from it. An additional difficulty is induced by the response time of the electrode which causes the $$P_{{\text{O}}_{\text{2}} }$$ measured to follow the real $$P_{{\text{O}}_{\text{2}} }$$ with a time lag large enough to significantly influence the measurements of the “critical $$P_{{\text{O}}_{\text{2}} }$$ ”. Because of these difficulties, the so measured “critical $$P_{{\text{O}}_{\text{2}} }$$ ” is not a function of the respiratory properties of cells and mitochondria but depends largely on the measuring device.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00587868
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Arteriovenous anastomoses in the cat carotid body (1973)
    Springer
    Cell & tissue research 142 (1973), S. 515-524 
    Details
    ISSN: 1432-0878
    Keywords: Arteriovenous anastomoses ; Carotid body ; Cat ; Corrosion casting technique ; Scanning electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Description / Table of Contents: Zusammenfassung In den arteriennahen, peripheren Bereichen des Glomus caroticum der Katze wurden arteriovenöse Verbindungen nachgewiesen, die ihrem Charakter nach unterschiedlich sind. Es ist allerdings noch nicht zu entscheiden, ob derartige Anastomosen regelmäßig vorkommen und wie sie funktionieren. Die beschriebenen Gefäßverbindungen konnten lichtmikroskopisch anhand von histologischen Serienschnitten und mit Hilfe einer weiterentwickelten Korrosionstechnik im Rasterelektronenmikroskop dargestellt werden.
    Notes: Summary In artery surrounding areas in the periphery of the carotid body of the cat we found arteriovenous anastomoses differing in respect to their character. So far, it is not yet to decide the frequency of their occurrence and their functional significance. The anastomoses were demonstrated by light microscopy of serial sections and by scanning electron microscopy with a more developed corrosion casting technique.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00306712
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    Paper (German National Licenses)
    Fulltext
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