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  • (Rabbit small intestine)  (2)
  • Electron microscopy  (2)
  • Erwinia carotovora subsp.atroseptica  (2)
  • Inorganic Chemistry  (2)
  • 1
    Electronic Resource
    Electronic Resource
    Amsterdam : Elsevier
    Biochimica et Biophysica Acta (BBA)/Biomembranes 731 (1983), S. 437-447 
    ISSN: 0005-2736
    Keywords: (Rabbit small intestine) ; Amino group ; Chemical modification ; Cotransport ; Glucose transport ; Na^+ transport ; Phlorizin binding ; Substrate-binding site
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology , Chemistry and Pharmacology , Medicine , Physics
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Amsterdam : Elsevier
    Biochimica et Biophysica Acta (BBA)/Biomembranes 900 (1987), S. 249-257 
    ISSN: 0005-2736
    Keywords: (Rabbit small intestine) ; Amino acid transport ; Brush-border membrane ; Carboxyl group ; Sodium ion cotransport ; Sugar transport
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology , Chemistry and Pharmacology , Medicine , Physics
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Amsterdam : Elsevier
    Biochimica et Biophysica Acta (BBA)/Gene Structure and Expression 696 (1982), S. 76-86 
    ISSN: 0167-4781
    Keywords: (Adenovirus) ; Chromatin ; Electron microscopy ; Nuclease digestion
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology , Chemistry and Pharmacology , Medicine , Physics
    Type of Medium: Electronic Resource
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  • 4
    ISSN: 1432-072X
    Keywords: Bradyrhizobium ; Electron microscopy ; Mutants ; Nitrogen fixation ; Nodulation ; Soybean ; Symbiosis ; Transposon Tn5
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The genome of the slow-growing Bradyrhizobium japonicum (strain 110) was mutagenized with transposon Tn5. A total of 1623 kanamycin/streptomycin resistant derivatives were screened in soybean infection tests for nodulation (Nod) and symbiotic nitrogen fixation (Fix). In this report we describe 14 strains possessing a stable, reproducible Nod+Fix- phenotype. These strains were also grown under microaerobic culture conditions to test them for free-living nitrogen fixation activity (Nif). In addition to strains having reduced Fix and Nif activities, there were also strains that had reduced symbiotic Fix activity but were Nif+ ex planta. Analysis of the genomic structure revealed that the majority of the strains had a single Tn5 insertion without any further apparent physical alteration. A few strains had additional insertions (by Tn5 or IS50), or a deletion, or had cointegrated part of the vector used for Tn5 mutagenesis. One of the insertions was found in a known nif gene (nifD) whereas all other mutations seem to affect different, hitherto unknown genes or operons. Several mutant strains had an altered nodulation phenotype, inducing numerous, small, widely distributed nodules. Light and electron microscopy revealed that most of these mutants were defective in different stages of bacteroid development and/or bacteroid persistence. The protein patterns of the mutants were inspected by two-dimensional gel electrophoresis after labelling microaerobic cultures with l-(35S)methionine. Of particular interest were mutants lacking a group of proteins the synthesis of which was known to be under oxygen control. Such strains can be regarded as potential regulatory mutants.
    Type of Medium: Electronic Resource
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  • 5
    ISSN: 1871-4528
    Keywords: Erwinia carotovora subsp.atroseptica ; effektive Dosis ; Vitalfärbung
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Description / Table of Contents: Summary The inoculum densities (effective dose) ofErwinia carotovora sub sp.atroseptica (Eca) needed to initiate infection of tuber tissue before and after a 24 h wound-healing period, was determined using a vital stain. The effects of various treatments on the efficiency of the defence reaction associated with wound healing, and on rot initiation were manifested as changes in the effective dose. The defence reaction was inhibited by temperatures 〈10°C, reduced O2 levels ≤5%, and CO2 proportions ≥20% (Fig. 3a and 4a). Moisture loss in stored tubers increased their defence reaction compared to that of freshly harvested ones (Table 1). The effect of these factors on Eca development was identical to that which occurs in potato production where tuber wounds are generally contaminated with Eca. Rot initiation was best prevented by removal of water (Table 1), storage at 15°C, avoidance of reduced O2 levels 〈10% and CO2 accumulation (Fig. 3b and 4b).
    Notes: Zusammenfassung Nach Inokulation mit abgestuften Bakteriendichten wurde über eine Vitalfärbung von Knollengewebe der Schwellenwert zur Fäuleauslösung bestimmt. Seine Erhöhung nach 24 h Wundheilung charakterisiert die Effizienz der biochemischen Fäuleabwehr. Die Abwehrreaktionen des Knollengewebes und der Prozess der Fäuleauslösung sind in unterschiedlichem Masse von der Temperatur, der Feuchtigkeit und dem O2- sowie dem CO2-Gehalt der Atmosphäre abhängig.
    Type of Medium: Electronic Resource
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  • 6
    ISSN: 1871-4528
    Keywords: Erwinia carotovora subsp.atroseptica ; effektive Dosis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Description / Table of Contents: Summary A cultivar collection was assessed for tuber resistance to the soft rot organism,Erwinia carotovora subsp.atroseptica (=Eca), and for the strength of the defence reaction associated with wound healing, by measuring the inoculum density needed to initiate infection (effective dose). Wound healing was more efficient in susceptible genotypes than in resistant ones (Fig. 1b), and resulted in changes of the ranking of cultivars, and a narrowing of the variation for soft rot resistance (Fig. 1a). No relationship was found between enzymic maceration of tissue and effective dose (Fig. 2a). The resistance ratings of cultivars were in general agreement with the effective dose and with symptom expression (Fig. 2b). Resistance behaviour was modified by growing conditions (harvest year) (Table 1), and cultivars need therefore to be assessed over several years.
    Notes: Zusammenfassung Die wundheilbedingte Infektionsabwehr beeinflusst das Resistenzverhalten der Knollen. Sie ist bei fäuleanfälligen Genotypen effizienter als bei widerstandsfähigen. Die Resistenzbewertung von Genotypen nach der Höhe der befallsauslösenden Erregerdichte wird von den Vegetationsbedingungen beeinflusst. Sie korreliert mit einer Bewertung nach dem Ausmass der Fäulesymptome, nicht aber mit dem Ausmass einer enzymatischen Mazeration.
    Type of Medium: Electronic Resource
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  • 7
    Electronic Resource
    Electronic Resource
    Weinheim : Wiley-Blackwell
    Berichte der deutschen chemischen Gesellschaft 20 (1887), S. 3336-3337 
    ISSN: 0365-9496
    Keywords: Chemistry ; Inorganic Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
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  • 8
    Electronic Resource
    Electronic Resource
    Weinheim : Wiley-Blackwell
    Berichte der deutschen chemischen Gesellschaft 20 (1887), S. 3118-3132 
    ISSN: 0365-9496
    Keywords: Chemistry ; Inorganic Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
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