ISSN:
1871-4528
Keywords:
α-chaconine
;
α-solanine
;
potato
Source:
Springer Online Journal Archives 1860-2000
Topics:
Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
Notes:
Summary A new, efficient and economic method employing Medium Pressure Liquid Chromatography (MPLC) for the isolation of the two majorSolanum tuberosum L. glycoalkaloids (α-solanine and α-chaconine) is described. Potato peelings are homogenised with 5% acetic acid, the glycoalkaloids purified by filtration through an XAD-2 column and then by precipitation from the aqueous solution. The resulting glycoalkaloid fraction was purified by MPLC using a Silica Gel column and a CHCl3:MeOH:2% NH4OH mixture (70∶30∶5) as mobile phase to yield pure α-chaconine and a-solanine. This methodology can be used to obtain glycoalkaloids for enthomology and toxicological research where large amounts of these compounds are required.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF02358001
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