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  • 11S acidic subunit  (1)
  • Brassica  (1)
  • 1
    Electronic Resource
    Electronic Resource
    Intervarietal and interspecific chimera formation by in vitro graft-culture method in Brassica (1992)
    Springer
    Theoretical and applied genetics 83 (1992), S. 727-732 
    Details
    ISSN: 1432-2242
    Keywords: Brassica ; Chimera ; Tissue culture ; Graft
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary An efficient chimera formation method by tissue culture combined with grafting was studied in Brassica. Cabbage cultivars “Ruby-ball” and “YR-ranpou” (Brassica oleracea) were used for intervarietal chimera formation. Seven-day-old seedlings leaving one of two cotyledons were approach-grafted and cultured in vitro (AGSC method). Chimeric shoots were obtained by the subculture of directly growing chimeric leaves (DGCL) from grafted part and cross-cut section of the united part after the graft culture. These were rooted and grown to complete chimera. An approach-grafted culture method was also available for interspecific chimera formation between “Komatsuna” (B. campestris) and “Ruby-ball” (B. oleracea), resulting in 20% formation per culture. Interspecific chimeras were identified as complex and peripheral-sectorial type by microscopic observation and the electrophoretic analysis of acid phosphatase isozyme pattern. The AGSC method was more effective than the usual Winkler's graft method and a simple mixed culture of heterogeneous cells or tissues.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00226691
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Graft-induced changes in soybean storage proteins. I. Appearance of the changes (1986)
    Springer
    Euphytica 35 (1986), S. 395-401 
    Details
    ISSN: 1573-5060
    Keywords: Glycine max ; soybean ; grafting ; storage protein ; 11S acidic subunit ; Kunitz trypsin inhibitor
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Genetic changes induced by grafting were observed in soybean storage proteins, involving the 11S acidic subunit composition and Kunitz trypsin inhibitor. Changes from Gly 1 Gly 1to Gly 1 gly 1involved the 11S acidic subunit, and those from Ti 0Ti0 to Ti 3Ti0 involved the KTI in the first progeny (G1S1) in the scion (Gly 1 Gly 1 Ti 0Ti0, var. Kinzu) grafted onto var. Raiden (gly 1 gly 1 Ti 3 Ti 3). The progenies G1S2 and G2S1 from the seeds that had been changed segregated for both proteins. However, the segregation ratio in the progenies were different from those of the F2's from the sexual crosses between Kinzu and Raiden used for grafting. These findings show that the changes (changed genes) were transmitted to the progenies in an unstable manner.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00021847
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    Paper (German National Licenses)
    Fulltext
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