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  • 13C-NMR spectroscopy  (2)
  • Acute myeloid leukemia  (2)
  • Genetics  (2)
  • 1
    Electronic Resource
    Electronic Resource
    Lymphoid subsets in acute myeloid leukemias: Increased number of cells with NK phenotype and normal T-cell distribution (1993)
    Springer
    Annals of hematology 67 (1993), S. 217-222 
    Details
    ISSN: 1432-0584
    Keywords: NK cells ; T cells ; AML ; Acute myeloid leukemia
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Natural killer (NK) and T subsets were analyzed with appropriate dual labeling by flow cytometry in peripheral blood (PB) (66 cases) and bone marrow (BM) (55 cases) from patients with de novo AML in order to determine: (a) their distribution at diagnosis, (b) the correlation between PB and BM in NK subpopulations, (c) their relationship with the clinical and hematological disease characteristics, and (d) the changes occurring upon achieving complete remission (CR). NK cells defined by the expression of CD56 in the absence of CD3 were significantly increased at diagnosis and their levels in PB correlated with those of BM. By contrast, NK subsets defined by CD16 expression (CD16+ CD2+ and CD16+ CD2− NK-cell subsets) as well as T lymphocytes with NK activity (CD56+ CD3+), although increased in PB, displayed normal levels in BM. An additional observation of interest was the expansion of an immature NK population lacking CD16 Ag expression (CD56+CD16−). AML cases were divided into two groups according to the absolute number of NK cells in PB; patients with the highest levels showed an increased proportion of blast cells in PB (p=0.01), monocytic subtypes (p=0.03), and expression of CD11b, CD14, and CD4 antigens (p=0.05). Infections at diagnosis were not related to the level of NK cells. In 19 patients who achieved complete remission the number of CD56+CD3− cells tended to be reduced to within the normal range. Other T-cell populations, including the CD4 naive and memory cells, were also explored, their distribution being normal in the PB of AML patients. By contrast, the cytotoxic subset CD8+/CD57+was significantly increased (p〈 0.001). These data point to the existence of marked alterations of NK cells in AML patients, possibly reflecting a host-tumor immunological interaction.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01715050
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  • 2
    Electronic Resource
    Electronic Resource
    The phenotype of L-CFU and its correlation with the immunological characteristics of the blast cell population in AML (1994)
    Springer
    Annals of hematology 68 (1994), S. 233-236 
    Details
    ISSN: 1432-0584
    Keywords: Immunophenotype ; L-CFU Clonogenic leukemic cell ; Acute myeloid leukemia
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The membrane phenotype of AML clonogenic cells (L-CFU) was analyzed in 19 AML patients using an in vitro culture technique after a complement-mediated lysis assay employing a panel of six monoclonal antibodies (McAb) -HLA-DR, FMC56 (CD9), FMC27 (CD9), CD14, CD15, CD41a-. Our results show that L-CFU has a heterogeneous but immature phenotype lacking on the expression of differentiation antigens (CD14, CD15, CD41a). In addition, we observed that the L-CFU phenotype is different from that of the whole blast cell population. Interestingly, L-CFU showed a higher expression of HLA-DR antigens with respect to their progeny. Upon analyzing whether the L-CFU phenotype was related to both the morphological and immunological features of AML blast cells, it was observed that, while there is no correlation with the FAB classification, there was a partial relationship between the immunological phenotype of AML blast cells and that of L-CFU. Accordingly, the more immature AML cases showed a more differentiated L-CFU phenotype (HLA-DR+, CD9+, FMC27+) when compared with cases with a more mature blast cell phenotype. These results suggest that those AML cases with a relatively immature myeloblastic phenotype may arise from a progenitor cell that has undergone partial differentiation and that is unable to acquire myeloid differentiation antigens, while those AML cases with mature blast cells might emerge from a very early L-CFU that has the capacity to undergo a greater degree of differentiation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01737422
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  • 3
    Electronic Resource
    Electronic Resource
    Synthesis of polyesters as binders for deinkable inks. VI. Structural analysis of the copolyesterification between o-phthalic anhydride, oleic acid, and trimethylolpropane in bulk at 160°C by high resolution 13C- and 1H nuclear magnetic resonance (13C- and 1H-NMR) (1997)
    Bognor Regis [u.a.] : Wiley-Blackwell
    Journal of Polymer Science Part A: Polymer Chemistry 35 (1997), S. 3409-3429 
    Details
    ISSN: 0887-624X
    Keywords: polyesters ; bulk copolyesterification ; o-phthalic anhydride ; oleic acid ; trimethylolpropane ; 13C-NMR spectroscopy ; 1H-NMR spectroscopy ; structural analysis ; trimethylolpropane esterification degree ; volumetry ; Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: A detailed structural analysis of the copolyesterification in bulk without any external catalyst at 160°C between o-phthalic anhydride (P), oleic acid (O), and trimethylolpropane (T) with a mol ratio ([—COOH]/[—OH]) = 0.70 has been carried out by high resolution 13C nuclear magnetic resonance (13C-NMR) (DMSO-d6 and CDCl3 solutions), 1H nuclear magnetic resonance (1H-NMR) (CDCl3 solution), and by volumetry. The use of CDCl3 as NMR solvent has allow us to identify several signals that have been assigned to trimethylolpropane monoesters with different esterification degrees in the o-phthalate residue. Identically, we have detected signals assignable to monoesters at the chain ends in structures with different chain lengths. These monoesterified structures have been also confirmed by analyzing samples modified by the diazomethane addition. These modified samples have been also used to determine free acid groups as their methoxylic derivatives by 1H-NMR in CDCl3 solution. We have not observed any detectable signs of gelation nor products produced by secondary reactions. © 1997 John Wiley & Sons, Inc. J Polym Sci A: Polym Chem 35: 3409-3429, 1997
    Additional Material: 8 Ill.
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    Synthesis of polyesters as binders for deinkable inks. I. Structural analysis of the polyesterification between o-phthalic anhydride and neopentyl glycol in bulk at 200°C by high resolution 13C nuclear magnetic resonance (13C-NMR) (1996)
    Bognor Regis [u.a.] : Wiley-Blackwell
    Journal of Polymer Science Part A: Polymer Chemistry 34 (1996), S. 1059-1072 
    Details
    ISSN: 0887-624X
    Keywords: polyesters ; bulk polyesterification ; o-phthalic anhydride ; neopentyl glycol ; 13C-NMR spectroscopy ; structural analysis ; Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: Analysis of the polyesterification in bulk without any external catalyst at 200°C of o-phthalic anhydride with neopentyl glycol (2,2-dimethyl-1,3-propanediol) with a mole ratio ([(SINGLE BOND) COOH]/[ (SINGLE BOND) OH]) = 0.7 has been carried out by high resolution 13C nuclear magnetic resonance (13C-NMR). Polyesters can be analyzed by 13C-NMR spectra because of the fact that both o-phthalic acid (o-phthalic anhydride) and neopentyl glycol carbons are sensitive to sequence effects. Spin-lattice relaxation times T1, of quaternary, tertiary and secondary carbons in different structures are in the 0.1-6.5 s range depending on the neighboring residue effects in the polymer chain. © 1996 John Wiley & Sons, Inc.
    Additional Material: 8 Ill.
    Type of Medium: Electronic Resource
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    Paper (German National Licenses)
  • 5
    Electronic Resource
    Electronic Resource
    VII. Yeast sequencing reports. The complete sequence of a 9000 bp fragment of the right arm of Saccharomyces cerevisiae chromosome VII contains four previously unknown open reading frames (1995)
    New York, NY [u.a.] : Wiley-Blackwell
    Yeast 11 (1995), S. 1087-1091 
    Details
    ISSN: 0749-503X
    Keywords: yeast genome ; chromosome VII ; Life and Medical Sciences ; Genetics
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: We report the sequence of a 9000 bp fragment from the right arm of Saccharomyces cerevisiae chromosome VII. Analysis of the sequence revealed four complete previously unknown open reading frames, which were named G7587, G7589, G7591 and G7594 following standard rules for provisional nomenclature. Outstanding features of some of these proteins were the homology of the putative protein coded by G7589 with proteins involved in transcription regulation and the transmembrane domains predicted in the putative protein coded by G7591. The sequence reported has been deposited in the EMBL data library under Accession Number X82775.
    Additional Material: 2 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/yea.320111110
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  • 6
    Electronic Resource
    Electronic Resource
    Molecular cloning of CIF1, a yeast gene necessary for growth on glucose (1992)
    New York, NY [u.a.] : Wiley-Blackwell
    Yeast 8 (1992), S. 183-192 
    Details
    ISSN: 0749-503X
    Keywords: CIF1 gene ; catabolite inactivation ; chromosome II ; S. cerevisiae ; Life and Medical Sciences ; Genetics
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: The cif1 mutation of Saccharomyces cerevisia (Navon et al., Biochemistry 18, 4487-4499, 1979) causes inability to grow on glucose and absence of catabolite inactivation. We have cloned the CIF1 gene by complementation of funcion and licated it in a 2·75 kb SphI-BstEII fragment situated at ca. 18 kb centomere distal of LYS2 and ca. 80 kb centromere proximal of TYRI on chromosome II. Southern analysis demostrated that CIF1 is present in a single copy in the yeast genome. Northern analysis revealed that the corresponding mRNA of 1·8 kb is more abundant in cells grown on galactose than in those grown on glucose. A protein of ca. 54 kDa was predicted from the open reading frame in the sequenced fragment. In strains carrying the cif1 mutation the intracellular concentration of ATP decreased immediately after addition of glucose while the intracellular concentration of cAMP did not increse. cAMP concentration increases in response to galactose or 2,4-dinitrophenol. Disruption of BCY1 or overexpression of CDC25 in a cif1/, background did not restore growth on glucose, suggesting that the absence of cAMP signal is not primary cause of lack of growth on glucose. Complementation tests showed that cif1 is not allelic to fdp1 although the two genes seem to be functionally related.
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/yea.320080304
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