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  • 1
    Electronic Resource
    Electronic Resource
    Springer
    International journal of legal medicine 102 (1989), S. 247-254 
    ISSN: 1437-1596
    Keywords: ABO grouping on hairs, immunohistochemistry ; Avidin-Biotin Complex, ABO grouping on hairs ; ABO-Blutgruppenbestimmung an Haaren, Immunhistochemie ; Avidin-Biotin-Komplex, ABO-Blutgruppenbestimmung an Haaren
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine , Law
    Description / Table of Contents: Zusammenfassung An 59 menschlichen Haarproben von Leichen und lebenden Personen wurden die ABO-Blutgruppen mittels der Avidin-Biotin-Technik untersucht. Eine positive Reaktion für A-, B- und H-Blutgruppen-Antigene konnte nur im Markstrang beobachtet werden. Wegen eines fehlenden Markstranges gelang der Nachweis in einigen Proben nicht. Bei 47 Haarproben von frischen Leichen und Lebenden stimmte das Untersuchungsergebnis mit der festgestellten Blutgruppe überein, aber einige Proben von Personen der Blutgruppe A2, Le(a+b−) zeigten eine schwache Reaktion mit Anti-A und eine starke mit Anti-H. Die Reaktion mit Anti-B und Anti-H bei einigen Fällen der Blutgruppe AB war stärker als mit Anti-A-Serum. Auch fünf Haarproben von fäulnisveränderten Leichen wurden untersucht. Bei zwei exhumierten und einer hochgradig fäulnisveränderten Leiche gelang der Blutgruppennachweis sicher, bei zwei Wasserleichen mit einer Liegezeit von 6 Monaten nicht. Von 28 Haarproben einer Blindstudie konnte in 22 Fällen ein positives Ergebnis erzielt werden, in sechs Fällen wegen des Fehlens eines Markstranges kein Ergebnis.
    Notes: Summary Fifty-nine hair specimens obtained from human autopsies and volunteers were used for the determination of ABO blood group substances using the ABC (Avidin-Biotin Complex) technique. Positive staining for A, B and H blood group substances was detected only in the medulla of the hairs. Blood group antigens could not be detected in seven hair specimens because they possessed no medulla. Forty-seven specimens obtained from fresh cadavers and volunteers gave the correct results corresponding to the blood group of the donor, but some specimens from individuals of blood group A2, Le(a+b−) showed weak reaction with anti-A and strong reaction with anti-H. The staining intensity with anti-B and -H in some individuals of blood group AB was stronger than with anti-A serum. Five hair specimens obtained from decomposed bodies were also examined. The blood group antigens could be specifically detected in hairs obtained from two exhumed and one putrid body, but no positive reactions were obtained from two cases of drowning where the bodies had been in the sea for about 6 months. In a blind trial, hair specimens from 28 individuals were also examined. Twenty-two specimens which possessed a medulla gave the correct result. Six specimens gave no result because they possessed no medulla.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    International journal of legal medicine 108 (1996), S. 323-326 
    ISSN: 1437-1596
    Keywords: Picoplankton ; Drowning ; 16S rDNA ; PCR
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine , Law
    Notes: Abstract Picoplankton belonging to theSynechococcus genus in cyanobacteria (approximately 1 μm in size) are found ubiquitously in Lake Biwa, Japan. However, they could not be morphologically discriminated from other bacteria by microscopy. In this study we attempted to use picoplankton for the diagnosis of drowning by PCR analysis of the 16S ribosomal DNA (rDNA). We designed primers complementary to the variable regions of 16S rDNA of the picoplankton we had sequenced. A comparison was made of the PCR products from the three picoplanktons, five other cyanobacteria,Melosira (diatom),Staurastrum (green alga), bacteria from Lake Baikal, and humans. The picogram order of template DNA from picoplankton was specifically amplified by the primers. When the template of picoplankton was mixed with human lung tissue, at least 10 ng of template DNA was needed to obtain a PCR product. The isolation of the picoplankton from human lung tissue increased the sensitivity of PCR more than a hundred-fold. The specific PCR products of the picoplankton were obtained from formalin-fixed drowning tissue. Molecular biological diagnosis of drowning was successful using picoplankton 16S rDNA.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    International journal of legal medicine 112 (1998), S. 72-74 
    ISSN: 1437-1596
    Keywords: Key words Multiplex STR profiling ; Automated DNA sequencer ; Romany (Gypsy) population ; Population ; genetics ; Hungary
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine , Law
    Notes: Abstract A population study of Hungarian Romanies was carried out on the STR loci HumLPL, HumF13B, HumFES and HumF13A01. There was little evidence for association of alleles within/between the four STR systems. Allele frequency distributions were significantly different between the Romany and the previously reported Central Hungarian population databases. Population differentiation was estimated by computing F- and º-statistics as well as frequency estimate differences of individual phenotypes for these two population samples. The results suggest that the population structure may have an effect on the interpretation of forensic DNA evidence in Hungary. Phylogenetic tree reconstruction with six populations from three major ethnic groups revealed a relatively distant genetic relationship of the Baranya Romanies with other Caucasian populations.
    Type of Medium: Electronic Resource
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