ISSN:
1432-203X
Keywords:
Keywords Kenaf
;
Hibiscus cannabinus L.
;
Shoot apical meristem
;
Multiple shoots
;
Tissue culture
;
""AbbreviationsBA 6-Benzyladenine
;
2,4-D 2,4-Dichlorophenoxyacetic acid
;
MS Murashige and Skoog inorganic salt medium
;
TDZ Thidiazuron (N-phenyl-N′-1,2,3-thiadiazol-5-ylurea)
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract In this research, a medium was developed that would stimulate multiple shoot initiation from shoot apex explants of Hibiscus cannabinus L. (kenaf). Adventitious shoot formation on a shoot induction media supplemented with combinations of 2,4-dichlorophenoxyacetic acid (2,4-D) (0, 0.5, 2.3 μmol·l–1) and thidiazuron (N-phenyl-N′-1,2,3-thiadiazol-5-ylurea; TDZ) (0, 1, 5, 20 μmol·l–1) was evaluated. Multiple shoot induction medium with 1 μmol·TDZ l–1 resulted in the highest number of regenerated shoots per explant for all three kenaf cultivars tested (Tainung 1, Tainung 2, and Everglades 71). The 2,4-D did not enhance multiple shoot formation. Additionally, kenaf cultivars 7N and SF459 also produced multiple shoots on the induction medium with 1 μmol·TDZ l–1. Multiple shoot clumps formed after 2 weeks in culture without callus formation. Shoots elongated and rooted within 2 weeks after subculture on a plant growth regulator-free medium. A histological study demonstrated the de novo regeneration of shoots from the shoot apex.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/s002990000256
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