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  • 1
    Electronic Resource
    Electronic Resource
    Behaviour of a replicating mitochondrial DNA sequence fromAspergillus amstelodami inSaccharomyces cerevisiae andAspergillus nidulans (1988)
    Springer
    Current genetics 13 (1988), S. 479-486 
    Details
    ISSN: 1432-0983
    Keywords: Amplified mitochondrial DNA ; ARS elements ; Transformation ; Aspergillus nidulans
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary An amplified sequence of mitochondrial DNA from a ragged (rgd) mutant ofAspergillus amstelodami has been shown to exist in multimeric circular form, suggesting that it is excised from the genome and can exist independently of it. This sequence has replicative (ARS) activity inSaccharomyces cerevisiae, and a subfragment responsible for this activity has been identified and sequenced. A homologous sequence fromAspergillus nidulans mtDNA also has ARS activity inS. cerevisiae. BothA. amstelodami andA. nidulans ARS elements have been incorporated into the integrative transformation vector pDJBI and the derived vectors used to transformA. nidulans. Inclusion of theA. nidulans ARS element enhanced the transformation frequency 5-fold relative to pDJBI. No increase in transformation frequency was evident with the ARS element fromA. amstelodami. The stability of transformants was variable but in comparison to pDJBI, ARS-containing plasmids were mitotically unstable inA. nidulans. Although plasmid DNAs could be rescued inEscherichia coli from undigested transformant DNA, no freely replicating plasmids were detected by Southern hybridisation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02427753
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Transformation of Penicillium chrysogenum using the Aspergillus nidulans amdS gene as a dominant selective marker (1987)
    Springer
    Current genetics 11 (1987), S. 639-641 
    Details
    ISSN: 1432-0983
    Keywords: Penicillium chrysogenum ; Transformation ; Acetamidase gene
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The Aspergillus nidulans acetamidase gene (amdS) has been used to transform Penicillium chrysogenum at low frequency. Several transformants were tested and shown to be mitotically stable. Southern blot analysis indicated that transforming DNA had integrated into the chromosomal DNA, possibly at multiple sites.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00393928
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    2-Phosphaindolizines (1991)
    Weinheim : Wiley-Blackwell
    Berichte der deutschen chemischen Gesellschaft 124 (1991), S. 475-480 
    Details
    ISSN: 0009-2940
    Keywords: 1,3-Azaphosphole, annulation to pyridine ; 2-Phosphaindolizines ; Pyridinium bromides, 1,2-dialkyl ; Phosphorus(III) chloride, condensation with CH2 groups ; Calculations, MNDO ; Chemistry ; Inorganic Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Additional Material: 1 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/cber.19911240309
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    Paper (German National Licenses)
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