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  • Analytical Chemistry and Spectroscopy  (14)
  • 25.70.Np  (4)
  • Immunofluorescence  (2)
  • 1
    Electronic Resource
    Electronic Resource
    Immunohistologische, feingewebliche und neurochemische Untersuchungen bei Encephalitiden (1968)
    Springer
    Acta neuropathologica 10 (1968), S. 74-81 
    Details
    ISSN: 1432-0533
    Keywords: Encephalitis ; Panencephalitis ; Immunofluorescence ; γ-Globulin ; Plasmocytic infiltration
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Zusammenfassung An perivasculären entzündlichen Infiltratzellen einer Panencephalitis wurde die Immunofluorescenz als Nachweis vom γ-Globulin in Plasmazellen untersucht und die fluorescenzoptischen Ergebnisse folgender vier verschiedener Gewebepräparationsmethoden verglichen: Formolfixierung, Modifikation der Albrechtschen Carnoy-Chloroformfixierung, Gefriertrocknung mit Carbowax-Einbettung und Gefrierschnitt-Technik an unfixierten Geweben. Die beste Immunofluorescenz bei gleichzeitiger guter Wahrung der strukturellen Gewebeformation wurde erzielt mit der modifizierten Carnoy-Chloroformfixierung und der Gefriertrocknung, während die Gefrierschnitt-Technik am unfixierten Gewebe zwar eine ausreichende Fluorescenz zeigte, aber in der Lokalisation des immunologischen Geschehens unzureichend war. Das formalinfixierte Gewebe war fluorescenzoptisch inaktiv. Als Methode der Wahl für die Fluorescenzmikroskopie an Hirngewebe wird die Gefriertrocknung mit Carbowax-Einbettung angesehen, da chemische Fixierungmittel nicht benutzt werden und daher denaturierte Eiweißstrukturen fluorescenzoptisch nicht zu erwarten sind.
    Notes: Summary In a case of panencephalitis the immunofluorescence technique was used to prove the presence of γ-globuline in plasmacells of perivascular infiltrations. The following four different brain tissue preparation methods were employed and the results compared: 1. formalin fixation, 2. modification of the Albrecht Carnoy-chloroform fixation, 3. lyophilisation with polyaethylenglycoll embedding, 4. frozen sections of unfixed tissue. The brightest staining and the utmost preservation of the tissue structure was obtained with the modified Carnoy-chloroform- and the lyophilisation techniques. The fluorescence in the frozen sections was sufficient, but the immunological reaction could often not be localized due to the loss of the tissue structure. The formalin treated tissue did not show any fluorescence. We consider the lyophilisation method with polyaethylenglycoll embedding for brain tissue as the best method since no chemical fixing agent is employed and therefore no unspecific staining resulting from denaturated protein structures is to be expected.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00690511
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  • 2
    Electronic Resource
    Electronic Resource
    Virus assembly inHincksia hincksiae (Ectocarpales, Phaeophyceae) An electron and fluorescence microscopic study (1998)
    Springer
    Protoplasma 203 (1998), S. 153-167 
    Details
    ISSN: 1615-6102
    Keywords: Algae ; Virus assembly ; DAPI staining ; Electron microscopy ; Hincksia hincksiae ; Immunofluorescence ; Marine double-stranded DNA virus
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The filamentous brown algaHincksia hincksiae can be infected by a large icosahedral double-stranded DNA virus (HincV-1). The virus shows extended latency and is replicated only in cells homologous to sporangia. Virus formation was studied by transmission electron microscopy, DAPI staining, and β-tubulin immunofluorescence. Inhibition of cytokineses results in multinucleate cells, which are the first indication of virus replication in productive cells; the microtubular cytoskeleton does not seem to be affected by the virus. Replication of viral DNA begins in the nuclei, which increase in size and eventually disintegrate. Virus assembly takes place in a mixed nucleo-/cytoplasm. Capsids bud from cisternae, which are interpreted as modified endoplasmic reticulum aggregated to virus assembly centres. The internal membranous component of the virus is thus derived from the endoplasmic reticulum. The particles are empty (electron translucent) when assembled, and the nucleoprotein core seems to be packaged subsequently through an opening in the capsid. A number of fine structural features not previously reported from brown algae and related to virus formation are described. Our results on Hincksia hincksiae virus are compared with observations made on various other icosahedral DNA viruses infecting eukaryotic algae and animals.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01279472
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  • 3
    Electronic Resource
    Electronic Resource
    β-Delayed neutron emission of15B,18C,19,20N,34,35Al and39P (1988)
    Springer
    The European physical journal 330 (1988), S. 63-68 
    Details
    ISSN: 1434-601X
    Keywords: 25.70.Np ; 23.40.-s ; 27.30.+ t
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract The isotopes15B,18C,19,20N,34,35Al and39P have been produced as projectile-like fragments from the interaction of a86Kr beam of 45 MeV/u from GANIL with a181Ta target. Separated by the 0° magnetic analyser LISE, the nuclei are identified by means of aΔE- E semiconductor telescope. It is surrounded, in a geometry close to 4π, by a thin plastic scintillator (NE 102) and a large volume liquid scintillator (NE 213) for the detection of betas and neutrons, respectively, from the decay of the implanted nuclei. By observation of theβ-delayed neutrons, the half-livesT 1/2 and neutron emission probabilitiesP n have been measured. TheT 1/2 of18C,20N,35Al,39P and Pn of18C,19,20N,34,35Al and39P are determined for the first time. For the isotopes17C,36,37Si and38P upper limits of Pn are deduced.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01287265
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  • 4
    Electronic Resource
    Electronic Resource
    Total cross sections of reactions induced by neutron-rich light nuclei (1989)
    Springer
    The European physical journal 332 (1989), S. 457-465 
    Details
    ISSN: 1434-601X
    Keywords: 21.10.-k ; 07.75.+h ; 21.10.Gv ; 25.70.Np ; 27.20.+n
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract Total nuclear reaction cross-sections are determined by means of a 4π-γ method. The results cover a wide span of targets for various stable beams. The validity of the method is shown in a combined systematics including also the results of transmission-type experiments. The data are very well described by the formula developed by Kox et al. The same method is applied to secondary fragment beams produced from a 44 MeV/u22Ne beam on a 332mg/cm2 181Ta target. Using the LISE spectrometer the fragments4, 6He,6–9, 11Li,7, 9–12, 14Be,10–15, 17B11–19C,13–19N,15–21O,18– 21F and20,21 Ne are analyzed and transported to interact with a 199.4 mg/cm2 Cu target surrounded by a 4π-γ counter. The measured total reaction cross-sectionsσ R are discussed in terms of the reduced strong absorption radiusr 0 and compared with other experimental results.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01292431
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  • 5
    Electronic Resource
    Electronic Resource
    Direct observation of new proton rich nuclei in the region 23≦Z≦29 using A 55A.MeV58Ni beam (1987)
    Springer
    The European physical journal 327 (1987), S. 17-24 
    Details
    ISSN: 1434-601X
    Keywords: 21.10-k ; 25.70.Np ; 27.40+z
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract Using magnetic separation and identification through time of flight andΔExE measurements, the nuclei56Cu,52Co (T z =−1);55Cu,51Co,47Mn,43V(T z =−3/2)52Ni,50Co,48Fe,46Mn,44Cr (T z =−2); and51Ni (T z =−5/2) are observed for the first time from interactions of 55A.MeV58Ni projectiles with a Ni target. The predicted proton drip-line has probably been reached for the odd elements.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01295244
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  • 6
    Electronic Resource
    Electronic Resource
    New neutron-rich isotopes in the scandium-to-nickel region, produced by fragmentation of a 500 MeV/u86Kr beam (1992)
    Springer
    The European physical journal 343 (1992), S. 67-71 
    Details
    ISSN: 1434-601X
    Keywords: 25.70.Np ; 27.50. + e
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract We have measured production cross-sections of the new neutron-rich isotopes58Ti,61V,63Cr,66Mn,69Fe,71Co and neighbouring isotopes that have been identified as projectile fragments from reactions between a 500 MeV/u86Kr beam and a beryllium target. The isotope identification was performed with the zero-degree magnetic spectrometer FRS at GSI, using in addition time-of-flight and energy-loss measurements. The experimental production cross-sections for the new nuclides and neighbouring isotopes are compared with an empirical parametrization. The resulting prospects for reaching even more neutron-rich isotopes, such as the doubly-magic nuclide78Ni, are discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01291599
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  • 7
    Electronic Resource
    Electronic Resource
    Direct stereochemical assignment of sugar subunits in naturally occurring glycosides by low energy collision induced dissociation. Application to papulacandin antibiotics (1988)
    Chichester : Wiley-Blackwell
    Biological Mass Spectrometry 15 (1988), S. 441-446 
    Details
    ISSN: 0887-6134
    Keywords: Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: A tandem mass spectrometric method is described which allows the assignment of stereochemistry to fragment ions comprising intact sugar subunits of larger glycosides without chemical degradation and product isolation by chromatography. The approach relies on the mass selection of the ‘sugar ion’ of interest followed by analysis of stereoselective fragmentation induced by low-energy collisional activation. The daughter ion spectra provide configurational fingerprints of the selected sugar ions which can be matched for identity with reference spectra obtained from suitable precursors of known stereochemistry. Glucose, mannose and galactose furnished the required set of the most important reference ions. By using peracetyl (and perdeuterioacetyl) derivatives, galactose was readily identified as the glycosidic sugar constituent of the (known) antibiotic papulacandin B and a further (unknown) congener.
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bms.1200150805
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  • 8
    Electronic Resource
    Electronic Resource
    High temperature (GC)2 and (GC)2/CI-MS of nucleosides: Analysis of purinyl nucleoside isomer composition (1980)
    Weinheim : Wiley-Blackwell
    Journal of High Resolution Chromatography 3 (1980), S. 161-165 
    Details
    ISSN: 0935-6304
    Keywords: GC, GC/MS ; Capillary, HMDS-deactivated ; On-column injection ; Nucleoside linkage isomers ; Separation, structural assignment feasibilities ; Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: The analysis of low-volatile polar nucleosides by (GC)2/FID and (GC)2/CI-MS, employing HMDS-deactivated glass capillary columns and on-column injection, is described. The four linkage isomers of a specific nucleoside, formed in the synthetic procedure, are in each case sufficiently well separated in the chromatogram; GC/CI-MS allows for a differentiation of N-7 and N-9 linkage isomers.
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jhrc.1240030402
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  • 9
    Electronic Resource
    Electronic Resource
    All-glass capillary T-joints and manifolds (1980)
    Weinheim : Wiley-Blackwell
    Journal of High Resolution Chromatography 3 (1980), S. 411-412 
    Details
    ISSN: 0935-6304
    Keywords: Gas chromatography ; Capillary, glass ; All-glass T-joints and manifolds with small dead volume, production of ; Up to five-channel outlet splitter ; Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Additional Material: 2 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jhrc.1240030806
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  • 10
    Electronic Resource
    Electronic Resource
    Tandem mass spectrometric analysis of fixed-charge derivatized oligosaccharidesPresented at the 12th IMSC, Amsterdam, August 1991. (1994)
    Chichester : Wiley-Blackwell
    Biological Mass Spectrometry 29 (1994), S. 713-719 
    Details
    ISSN: 0030-493X
    Keywords: Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: Various derivatives of a set of three isomeric (linear and branched) pentasaccharides (LNF-1, LNF-2 and LNF-3) were prepared and investigated by high-performance tandem mass spectrometry in order to compare their collision-induced dissociation (CID) behaviour. The fast atom bombardment tandem mass spectra of [M + H]+ ions of peracetylated derivatives mainly display fragments containing the non-reducing terminus (B-type ions) and allow a straightforward assignment of sugar sequence and branching together with the identification of some interglycosidic linkages. Permethylated derivatives, which better accommodate the mass range of tandem mass spectrometers in the case of larger oligosaccharides, yield similar results, i.e. predominance of B ions, but the necessary information has to be retrieved from incomplete Bi and Yi ion series. By contrast, CID of permethyl or peracetyl derivatives carrying a preformed charge due to prior reductive amination of the oligosaccharides with trimethyl(p-aminophenyl)ammonium chloride yields exclusively fragment ions comprising the reducing end. In this case, the four distinct series of fragments observed involve charge-remote fragmentation processes. As a consequence, the spectral patterns are not significantly affected by the nature of the sugar O-substituents additionally introduced (i.e. methyl or acetyl).
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/oms.1210291204
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