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  • 27.80.+w 190 ⩽ A ⩽ 219  (1)
  • Key wordsKluyveromyces lactis   (1)
  • LGT1  (1)
  • 1
    ISSN: 1617-4623
    Keywords: Key wordsKluyveromyces lactis  ;  Casein kinase  ;   Glucose transport  ;  Transcriptional regulation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The RAG8 gene of Kluyveromyces lactis, which is one of the genes controlling the expression of the low-affinity carrier gene RAG1, has been cloned by in vivo complementation of the rag8 mutation. The sequence of Rag8p (535 amino acids), deduced from the nucleotide sequence of the cloned RAG8 gene, has been found to share a high degree of identity with the two casein kinases I of Saccharomyces cerevisiae, Yck1p and Yck2p, encoded by YCK1 and YCK2: the proteins are 65–66% identical overall and show 89–90% identity in the kinase domain. The finding that the RAG8 gene of K. lactis cloned in a centromeric vector was able to complement the growth defect of a yck1Δyck2 ts mutant of S. cerevisiae strongly suggested that Rag8p is a casein kinase I. In contrast to the S. cerevisiae homologs, the RAG8 gene of K. lactis seems to be an essential single-copy gene, as shown by Southern blot experiments and the lethality of the rag8 null mutation. Northern blot analysis showed that the transcription of the RAG8 gene was higher on glucose media than in cells grown on a non-fermentable carbon source.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1434-601X
    Keywords: 27.80.+w 190 ⩽ A ⩽ 219 ; 25.40.-h Nucleon-induced reactions ; 25.40.Sc Spallation reactions
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract. Neutrons and light charged particles produced in 62.9MeV proton-induced reactions on 208Pb were measured during a single experiment performed at the CYCLONE facility in Louvain-la-Neuve (Belgium). Two independent experimental set-ups were used to extract double differential cross-sections for neutrons, protons, deuterons, tritons, 3He and alpha-particles. Charged particles were detected using a set of Si- Si- CsI telescopes from 25° to 155°, by step of 10 degrees. Neutrons were measured using shielded DeMoN counters, liquid NE213 scintillators, at 24°, 35°, 55°, 80° and 120°. These data allowed the determination of angle differential, energy differential and total production cross-sections. A comparison with theoretical calculations (MCNPX, FLUKA and TALYS) has been performed. It shows that the neutron and proton production rates are well predicted by MCNPX, using the INCL4 option. All the other codes underestimate the neutron production whereas they overestimate the proton one. For composite particles, which represent 17% of the charged particle total reaction cross-section, neither the shape nor the amplitude of the cross-sections are correctly predicted by the models.
    Type of Medium: Electronic Resource
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  • 3
    ISSN: 0749-503X
    Keywords: glucose transport ; Saccharomyces cerevisiae ; LGT1 ; Life and Medical Sciences ; Genetics
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: A low-affinity glucose transporter gene of Saccharomyces cerevisiae was cloned by complementation of the rag1 mutation in a strain of Kluyveromyces lactis defective in low-affinity glucose transport. Gene sequence and effects of null mutation in S. cerevisiae were described. Data indicated that there are multiple genes for low-affinity glucose transport.
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
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