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  • Rice  (8)
  • Glycosphingolipid  (3)
  • 3-D image  (2)
  • 1
    Digitale Medien
    Digitale Medien
    Amsterdam : Elsevier
    Biochimica et Biophysica Acta (BBA)/Lipids and Lipid Metabolism 666 (1981), S. 426-432 
    ISSN: 0005-2760
    Schlagwort(e): (Hamster fibroblast) ; CMP-N-acetylneuraminic acid ; Glycosphingolipid ; Glycosyltransferase ; UDP-N-acetylgalactosamine
    Quelle: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Thema: Biologie , Chemie und Pharmazie , Medizin , Physik
    Materialart: Digitale Medien
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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  • 2
    Digitale Medien
    Digitale Medien
    Amsterdam : Elsevier
    Biochimica et Biophysica Acta (BBA)/Lipids and Lipid Metabolism 666 (1981), S. 418-425 
    ISSN: 0005-2760
    Schlagwort(e): (Hamster fibroblast) ; Glycosphingolipid ; Glycosyltransferase ; UDPgalactose ; UDPglucose
    Quelle: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Thema: Biologie , Chemie und Pharmazie , Medizin , Physik
    Materialart: Digitale Medien
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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  • 3
    Digitale Medien
    Digitale Medien
    Springer
    Surgical and radiologic anatomy 20 (1998), S. 291-297 
    ISSN: 1279-8517
    Schlagwort(e): Skull ; Craniometry ; Anthropology ; 3-D image ; Helical CT
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Abstract To make a digital image database of human craniology, we optimized the three-dimensional (3-D) images of 29 dried human skull specimens by helical computed tomography (CT). For the verification of the quantitative exactitude of these image data, we manually measured nine items of direct distances between standard anthropologic points on each skull and the corresponding distances projected on the CT monitor by specifying the respective points. The results obtained by the two methods of manual and CT measurements were compared and statistically analyzed. The CT measurements were so exact that the lower limit of correlation coefficients (95% of the confidence interval) between the two results was more than 0.8 in six items; i.e., maximal cranial length and breadth, minimal frontal breadth, bizygomatic breadth, distance between ectomolares and nasion-basion length. In contrast, the CT results were less well correlated with the manual measurements of three items; i.e., distance between bilateral mastoidales, total facial height, and nasal breadth. We concluded that the qualitative representation of 3-D CT images was adequate, although some quantitative data may be incorrect. The inaccuracy is suspected to be due to the difficulty in specifying the standard points on the CT images, and due to the differences in measurement procedures between the direct and projected distances.
    Materialart: Digitale Medien
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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  • 4
    Digitale Medien
    Digitale Medien
    Springer
    Cellular and molecular life sciences 46 (1990), S. 611-616 
    ISSN: 1420-9071
    Schlagwort(e): Glycosphingolipid ; topography of glycolipid glycosylation ; the Golgi apparatus ; glucosylceramide ; monensin ; glycolipid transfer protein
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary Glycolipid transfer protein (GL-TP), a nonglycosylated protein with a molecular weight of 22,000 K, has been purified from pig brain. The protein transfers, by a carrier mechanism, glycolipids with a β-glucosyl or β-galactosyl residue directly linked to either ceramide or diacylglycerol. GL-TP appears to be present in most animal cells, and evidence has been obtained which indicates that it is a cytoplasmic protein. Little is known about the function of GL-TP. Current evidence indicates that glycosphingolipid glycosylation occurs at the luminal side of the Golgi apparatus, except for the glucosylation of ceramide, which has been shown to occur at the cytoplasmic side of the Golgi or endoplasmic membrane. It appears most likely that GL-TP participates in the intracellular traffic of glucosylceramide.
    Materialart: Digitale Medien
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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  • 5
    Digitale Medien
    Digitale Medien
    Springer
    Surgical and radiologic anatomy 20 (1998), S. 291-297 
    ISSN: 1279-8517
    Schlagwort(e): Skull ; Craniometry ; Anthropology ; 3-D image ; Helical CT
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Beschreibung / Inhaltsverzeichnis: Résumé Afin d'établir une banque informatisée de données en crâniologie humaine, nous avons recueilli les images tridimensionnelles, de 29 crânes secs, obtenues par scanner hélicoïdal. Pour vérifier les données obtenues, nous avons mesuré manuellement 9 longueurs situées entre les repères crâniologiques classiques sur chaque crâne et les distances correspondantes entre les points analogues sur la console du scanner. Les résultats obtenus par les 2 méthodes de mesure manuelle et par scanner sont comparés et analysés statistiquement. Les mesures scanner sont situées à la limite inférieure de corrélation entre les 2 résultats (95% d'intervalle de confiance) et supérieures à 0.8 dans 6 mesures : la longueur et la largeur maximales crâniennes, la largeur minimale frontale, la largeur bizygomatique, la distance entre les faces externes des molaires et la longueur nasion-basion. Par contre, les mesures scanner sont moins concordantes avec les résultats manuels dans 3 mesures : distance intermastoïdienne, hauteur faciale totale et largeur nasale. Nous en concluons que la représentation qualitative des images scanner est correcte, même si quelques données chiffrées sont imprécises. Les causes d'erreurs sont, semble t-il, dues à la difficulté de repérer les points crâniologiques précis sur les images scanner, ainsi qu'à la différence des techniques de mesure entre une donnée directe et une en projection.
    Notizen: Summary To make a digital image database of human craniology, we optimized the three-dimensional (3-D) images of 29 dried human skull specimens by helical computed tomography (CT). For the verification of the quantitative exactitude of these image data, we manually measured nine items of direct distances between standard anthropologic points on each skull and the corresponding distances projected on the CT monitor by specifying the respective points. The results obtained by the two methods of manual and CT measurements were compared and statistically analyzed. The CT measurements were so exact that the lower limit of correlation coefficients (95% of the confidence interval) between the two results was more than 0.8 in six items; i.e., maximal cranial length and breadth, minimal frontal breadth, bizygomatic breadth, distance between ectomolares and nasion-basion length. In contrast, the CT results were less well correlated with the manual measurements of three items; i.e., distance between bilateral mastoidales, total facial height, and nasal breadth. We concluded that the qualitative representation of 3-D CT images was adequate, although some quantitative data may be incorrect. The inaccuracy is suspected to be due to the difficulty in specifying the standard points on the CT images, and due to the differences in measurement procedures between the direct and projected distances.
    Materialart: Digitale Medien
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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  • 6
    ISSN: 1432-2242
    Schlagwort(e): Key words RFLP markers ; Days-to-heading ; QTL analysis ; Rice ; Epistatic interaction
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract  Quantitative trait locus (QTL) analysis has been carried out to identify genes conferring heading date in rice. One hundred and eighty six F2 plants derived from a cross between a japonica variety, Nipponbare, and an indica variety, Kasalath, were used as a segregating population for QTL mapping and more than 850 markers were employed to identify QTLs. Scan-analysis revealed the existence of two QTLs with large effects, Hd-1 and Hd-2, one in the middle of chromosome 6 and one at the end of chromosome 7, respectively. For both loci, the Kasalath alleles reduced days-to-heading. In addition, three QTLs with minor effects, Hd-3, Hd-4 and Hd-5, were found to be located on chromosomes 6, 7 and 8 based on a secondary scan analysis which was carried out by removing the phenotypic effects of Hd-1 and Hd-2. For the three secondary loci, the Nipponbare alleles reduced days-to-heading. The five QTLs explained 84% of the total phenotypic variation in the F2 population based on a multiple-QTL model. The presence of a digenic interaction between Hd-1 and Hd-2 was clearly suggested.
    Materialart: Digitale Medien
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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  • 7
    ISSN: 1432-2242
    Schlagwort(e): STS ; RFLP ; Rice ; Genetic map ; Coding region
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract Generating sequence-tagged sites (STSs) is a prerequisite to convert a genetic map to a physical map. With the help of sequence information from these STSs one can also isolate specific genes. For these purposes, we have designed PCR primer sets, of 20 bases each, by reference to sequences of restriction fragment length polymorphism (RFLP) landmarkers consisting of rice genomic clones. These markers were evenly distributed over the 12 chromosomes and were shown to be single copy by Southern-blot analysis. With improved PCR protocols, 63 standard STS landmarkers in the rice genome were generated. Similarity searches of all partial sequences of RFLP landmarkers by the FASTA algorithm showed that 2 of the 63 RFLP landmarkers, G357 and G385, contained part of the ORFs of aspartate aminotransferase and protein kinase, respectively.
    Materialart: Digitale Medien
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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  • 8
    ISSN: 1432-2242
    Schlagwort(e): Gametophyte gene ; Genetic map ; Hybrid sterility gene ; Rice ; Segregation distortion
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract We have constructed a high-resolution rice genetic map containing 1383 DNA markers covering 1575 cM on the 12 linkage groups of rice using 186 F2 progeny from a cross between a japonica variety, ‘Nipponbare’, and an indica variety, ‘Kasalath’. Using this high-resolution molecular linkage map, we detected segregation distortion in a single wide cross of rice. The frequencies of genotypes for 1181 markers with more than 176 genotype data were plotted along this map to detect segregation distortion. Several types of distorted segregation were observed on 6 of the chromosomes. We could detect 11 major segregation distortions at ten positions on chromosomes 1, 3, 6, 8, 9, and 10. The strongest segregation distortion was at 107.2 cM on chromosome 3 and may be the gametophyte gene 2 (ga-2). The ‘Kasalath’ genotype at this position was transmitted to the progeny with about a 95% probability through the pollen gamete. At least 8 out of the 11 segregation distortions detected here are new. The use of the high-resolution molecular linkage map for improving our understanding of the genetic nature and cause of these segregation distortions is discussed.
    Materialart: Digitale Medien
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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  • 9
    ISSN: 1432-2242
    Schlagwort(e): Key words Regeneration ability ; QTL ; Rice ; Oryza sativa L. ; Seed callus
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract  Quantitative trait loci (QTL) controlling the regeneration ability of rice seed callus were detected using 245 RFLP markers and 98 BC1F5 lines derived from two varieties, ‘Nipponbare’ and ‘Kasalath’. Regeneration ability was evaluated by two indices: average number of regenerated shoots per callus (NRS) and regeneration rate (RR). The BC1F5 lines showed continuous segregation for both indices. Five putative QTL for NRS (tentatively named qRg1, qRg2, qRg4a, qRg4b and qRg4c) located on chromosomes 1, 2 and 4 were detected. Digenic interaction among these detected QTL was not significant (P〈0.01). Among the five QTL detected, four ‘Kasalath’ alleles and one ‘Nipponbare’ allele increased NRS. According to an estimate based on the nearest marker loci, the five QTL accounted for 38.5% of the total phenotypic variation of the BC1F5 lines. For RR, four putative QTL were detected on chromosomes 2 and 4, and all of these were in the same chromosomal regions as the NRS QTL. The four RR QTL accounted for 32.6% of the total phenotypic variation.
    Materialart: Digitale Medien
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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  • 10
    ISSN: 1432-2242
    Schlagwort(e): Plant disease resistance ; Rice ; Xanthomonas oryzae pv. oryzae ; YAC
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract Map-based cloning methods have been applied for isolation of Xa-1, one of the bacterial blight resistance genes in rice.Xa-1 was previously mapped on chromosome 4 using molecular markers. For positional cloning of Xa-1, a high-resolution genetic map was made for theXa-1 region using an F2 population of 402 plants and additional molecular markers. Three restriction fragment length polymorphism (RFLP) markers, XNpb235, XNpb264 and C600 were found to be linked tightly to Xa-1, with no recombinants, and U08 750 was mapped 1.5 cM from Xa-1. The screening of a yeast artificial chromosome (YAC) library using theseXa-1-linked RFLP markers resulted in the identification of ten contiguous YAC clones. Among these, one YAC clone, designated Y5212, with an insert of 340 kb, hybridized with all three tightly linked markers. This YAC was confirmed to possess the Xa-1 allele by mapping the Xa-1 gene between both end clones of this YAC (Y5212R and Y5212L).
    Materialart: Digitale Medien
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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