ISSN:
1432-2307
Keywords:
Glomerular epithelial cell culture
;
Monocyte
;
Tumour necrosis factor α
;
Interleukin 1 β
;
MTT assay
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Abstract Rat glomerular epithelial cells were cultured with human monocyte supernatant or with recombinant cytokines. A primary glomerular culture and a glomerular epithelial cell culture were made; supernatant from monocyte cultures derived from healthy humans, and recombinant tumour necrosis factor α (TNF α) or recombinant interleukin 1 β (IL-1 β) were added. Cell proliferation rates were assayed by the MTT (3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide) assay. In serum-free media, consistent proliferation of glomerular epithelial cells (GEC) was observed throughout the 3 week culture period. Significant growth-stimulatory effects were induced by lipopolysaccharide-treated monocyte conditioned medium and by 1–50 ng/ml of TNF α, growth being up to 400% more than in the control culture. The effect of TNF α depended mainly on its interaction with epidermal growth factor (EGF). In contrast to TNF α, IL-1 β inhibited GEC proliferation; this was due to the early appearance and proliferation of mesangial cells, despite the culture being serum-free. This study showed that activated monocytes secrete growth factors for GEC in vitro, and that interaction between both TNF α and IL-1 β and between TNF α and EGF can modulate GEC proliferation. These findings suggest that, under pathological conditions, monocytes or macrophages affect GEC proliferation, probably being involved in crescent formation.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00195770
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