ISSN:
1432-2242
Keywords:
Valine resistance
;
Nicotiana plumbaginifolia
;
Protoplast
;
Amino acid uptake
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Summary Haploid mesophyll protoplasts of Nicotiana plumbaginifolia were mutagenized by UV-irradiation. Protoplast-derived colonies were then selected for valine resistance on a medium containing 5 or 10 mM valine. From the resistant calli, plants were regenerated. Resistance was inherited as a recessive Mendelian character in seven clones. Mutations conferring valine resistance were shown to be allelic. Protoplast-derived cells of L-valine-resistant plants were also resistant to L-threonine. Resistance to valine was based on a reduced valine uptake rate.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00303963
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