Electronic Resource
Oxford, UK
:
Blackwell Publishing Ltd
Physiologia plantarum
79 (1990), S. 0
ISSN:
1399-3054
Source:
Blackwell Publishing Journal Backfiles 1879-2005
Topics:
Biology
Notes:
Liposomes spontaneously interact with the plasma membrane of protoplasts. The delivery of their content to the protoplast, however, has to be induced by physical or chemical treatments. Three major approaches have been used to study and optimize the delivery of RNA or DNA molecules: viral infection, transient gene expression and stable transformation. Conclusive evidence for the delivery of nuclei acids to the treated protoplasts has been obtained. Stable transformants expressing kanamycin resistance have been further characterized by hybridization techniques and progeney analysis. The potential and limitations of this approach for the transformation of higher plants is discussed.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1111/j.1399-3054.1990.tb05882.x
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