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  • 1
    ISSN: 1432-0649
    Keywords: 42.60 ; 33.20
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract A computer-controlled diode laser spectrometer for the 1200 to 2500 cm−1 spectral region is described. The spectrometer has been applied to high resolution spectroscopy of the NCO radical at 5.2 μm. The lead-salt diode lasers are cooled to their operating temperature with a temperature adjustable helium evaporation cryostat. Computer-controlled tuning procedures for the frequency tuning of the diode lasers have been developed; they are independent of tables describing the tuning characteristics of the diode lasers. 41 lines of the antisymmetric stretching-vibrationv 3 of the linear NCO radical have been observed. We were able to detect vibration-rotation transitions in both2 π 1/2 and2 π 3/2 fine structure sublevels. These measurements led to the precise determination of additional molecular constants.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1432-0428
    Keywords: Diabetes ; uninephrectomy ; islet transplantation ; glomerulus ; mesangium ; basement membrane
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Uninephrectomy is known to accelerate the development of both functional and morphological changes seen with experimental diabetic nephropathy in the rat. The present experiments utilized electron and light microscopic morphometric techniques to assess glomerular basement membrane width and the volumes of the total mesangium and its cellular and matrix components of inbred Lewis rats made diabetic at 6 weeks of age and uninephrectomized 9 days later. Immunofluorescent microscopy was used to evaluate IgG and C3 in the mesangium. The reversibility of established diabetic glomerular lesions in uninephrectomized diabetic rats after 7 months of diabetes was studied by performing intraportal transplant of neonatal pancreatic tissue. Renal biopsies were taken 2 months later in transplanted and non-transplanted animals. Islet transplantation lowered plasma glucose to normal levels (29.6 to 7.3 mmol/l) and raised plasma insulin values (6.3 to 53 μU/l). Glomerular basement membrane width in transplanted rats (268 nm) still exceeded the same measure (226 nm) in nondiabetic uninephrectomized rats. In transplanted animals volumes of the mesangium (0.51 × 106 μm3) and of its cellular (0.27 × 106 μm3) and matrix (0.24 × 106 μm3) components remained higher than similar measures in control rats (0.32 × 106, 0.17 × 106 and 0.15 × 106 μm3, respectively). Mesangial IgG in treated animals approached normal, but mesangial C3 remained similar to levels in non-transplanted diabetic control animals. These observations in uninephrectomized-diabetic rats contrast with previous observations in intact diabetic rats in which mesangial volumes and localization of immunoglobulins and complement returned to normal levels following islet transplantation.
    Type of Medium: Electronic Resource
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  • 3
    ISSN: 1432-0428
    Keywords: Kidney size ; diabetic nephropathy ; creatinine clearance ; mesangium ; glomerular basement membrane ; urinary albumin excretion ; hypertension ; Type 1 diabetes
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Thirty-five patients with Type 1 (insulin-dependent) diabetes mellitus and 90 normal subjects had renal size (renal area index) determined by X-ray and also had examination of renal biopsies by light and electron microscopy. Renal area index of 206±32 cm2/1.73 m2 (mean±SD) in the Type 1 diabetic patients exceeded that in the normal subjects (180±25 cm2/1.73 m2, p〈0.001). In the diabetic patients, the renal area index correlated with creatinine clearance (r=+0.43, p〈0.05), but did not correlate with urinary albumin excretion, or the electron microscopic measurements of percentage total mesangium and glomerular basement membrane width. In diabetic patients with clinical nephropathy or severe glomerulopathy on biopsy, the kidneys may remain large. Thus, renal size does not indicate the severity of diabetic renal lesions on biopsy.
    Type of Medium: Electronic Resource
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