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  • 42.80  (4)
  • Atomic, Molecular and Optical Physics  (3)
  • Physics  (3)
  • reconstitution  (2)
  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Applied physics 32 (1983), S. 187-191 
    ISSN: 1432-0649
    Keywords: 42.80
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract A split detector is used in a scanning optical microscope to produce high-quality differential amplitude contrast images. A slight lateral offset in the detector position is shown to introduce information about object height variations to the image. These results are compared with images obtained by electrical differentiation.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Applied physics 49 (1989), S. 431-433 
    ISSN: 1432-0649
    Keywords: 42.40 ; 42.65 ; 42.80
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract The emergence of higher diffracted orders when a slightly wedged BSO crystal is illuminated by a single coherent beam is studied with input power and applied voltage as parameters. It is shown that the power spreads gradually towards the higher orders and that the temporal variation of the power in each beam is qualitatively the same as in the case of two-wave interaction.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    Applied physics 27 (1982), S. 211-213 
    ISSN: 1432-0649
    Keywords: 06 ; 42.80
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract The use of the depth discriminination property of the confocal scanning microscope for surface profiling has been adapted to provide a method of high-resolution three-dimensional surface profilometry. Measurements on a semiconductor specimen demonstrate the technique; depth variations of the order of 0.1 μm are clearly resolved.
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    Springer
    Applied physics 22 (1980), S. 119-128 
    ISSN: 1432-0630
    Keywords: 42.30 ; 42.80 ; 72.40
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Notes: Abstract This review paper is concerned with the imaging properties and major uses of scanning optical microscopes. It is shown that the confocal scanning microscope exhibits a form of super-resolution and that the instrument in general has great application in nonlinear microscopy and the inspection of electronic devices.
    Type of Medium: Electronic Resource
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  • 5
    Electronic Resource
    Electronic Resource
    Springer
    The journal of membrane biology 79 (1984), S. 185-193 
    ISSN: 1432-1424
    Keywords: reconstitution ; lactose transport ; membrane potential ; pH gradient ; proteolytic enzymes
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Summary The lactose transport carrier from parental (X71/F'W3747) and mutant cells (54/F'5441) was reconstituted into proteoliposomes. Transport by the counterflow assay showed slightly greater activity in proteoliposomes prepared from extracts of the mutant membranes compared with that for the parental cell. The mutant carrier showed a threefold lowerK m but similarV max compared to the parent. On the other hand proteoliposomes from the mutant showed a defect in protonmotive force-driven accumulation, compared with the parent. With a pH gradient (inside alkaline) plus a membrane potential (inside negative) the parental proteoliposomes accumulated lactose 25-fold over the medium concentration while the mutant proteoliposomes accumulated sixfold. In a series of experiments proteoliposomes were exposed to proteolytic enzymes. Chrymotrypsin treatment resulted in 30% inhibition of counterflow activity for the reconstituted carrier from both parent and mutant. Papain produced 84% inhibition of transport by the reconstituted parental carrier but only 41% of that of the mutant. Trypsin and carboxypeptidase Y treatment had no effect on counterflow activity of either parent or mutant. Exposure of purified lactose carrier in proteoliposomes to carboxypeptidase Y resulted in the release of alanine and valine, the two C-terminal amino acids predicted from the DNA sequence.
    Type of Medium: Electronic Resource
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  • 6
    ISSN: 1432-1424
    Keywords: proteoliposomes ; counterflow ; lactose carrier ; phospholipid requirement ; Escherichia coli ; reconstitution
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Summary The lactose carrier was extracted from membranes ofEscherichia coli and transport activity reconstituted in proteoliposomes containing different phospholipids. Two different assays f for carrier activity were utilized: counterflow and membrane potential-driven uptake. Proteoliposomes composed ofE. coli lipid or of 50% phosphatidylethanolamine−50% phosphatidylcholine showed very high transport activity with both assays. On the other hand, proteoliposomes containing asolectin, phosphatilcholine or 25% cholesterol/75% phosphatidylcholine showed good counterflow activity but poor membrane potentialdriven uptake. The discrepancy between the two types of transport activity in the latter group of three lipids is not due to leakiness to protons, size of proteoliposomes, or carrier protein content per proteoliposome. Apparently one function of the carrier molecule shows a broad tolerance for various phospholipids, while a second facet of the membrane protein activity requires very restricted lipid enviroment.
    Type of Medium: Electronic Resource
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  • 7
    Electronic Resource
    Electronic Resource
    New York, NY : Wiley-Blackwell
    International Journal of Quantum Chemistry 44 (1992), S. 703-716 
    ISSN: 0020-7608
    Keywords: Computational Chemistry and Molecular Modeling ; Atomic, Molecular and Optical Physics
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: We report on the results of calculations of the electronic structure of α-quartz that were made using the first principles, factorized linear combination of atomic orbitals method. Results were obtained for the primitive 9-atom, and orthorhombic 18- and 72-atom unit cells. Application of this method to the calculation of the electronic structure of the neutral oxygen vacancy in α-quartz is discussed and results obtained using a 72-atom unit cell are given. © 1992 John Wiley & Sons, Inc.
    Additional Material: 9 Ill.
    Type of Medium: Electronic Resource
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  • 8
    Electronic Resource
    Electronic Resource
    New York : Wiley-Blackwell
    Journal of Polymer Science: Polymer Physics Edition 12 (1974), S. 1607-1618 
    ISSN: 0098-1273
    Keywords: Physics ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Physics
    Notes: The dynamic mechanical properties have been determined and the β relaxation temperatures Tβ have been located for a series of copolymers of ethylene with norbornene, methylnorbornene, dicyclopentadiene, and octahydrodimethanonaphthalene. The Tβ values show discontinuous changes with changing comonomer content. The several levels of Tβ observed apparently can be associated with the sequence of close multiplets of norbornane moieties ranging from isolated units through pairs to triads and higher. For isolated norbornane units Tβ is around 10°C, while for pairs of these units, probably separated by a C2H4 unit, it is 55°C. The size of the norbornene-type comonomer has a small but measurable effect on these values of Tβ. The bearing of these results on the problem of defining a value of Tg for polyethylene is discussed.
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
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  • 9
    Electronic Resource
    Electronic Resource
    New York, NY : Wiley-Blackwell
    International Journal of Quantum Chemistry 44 (1992), S. 687-701 
    ISSN: 0020-7608
    Keywords: Computational Chemistry and Molecular Modeling ; Atomic, Molecular and Optical Physics
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: We report on the results of electronic band structure calculations of bulk ZnSe, bulk ZnS, and the (ZnSe)1(ZnS)1 strained-layer superlattice (SLS) using the ab initio factorized linear combination of atomic orbitals method. The bulk calculations were done using the standard primitive nonrectangular 2-atom zincblende unit cell, while the SLS calculation was done using a primitive tetragonal 4-atom unit cell modeled from the CuAu I structure. The analytic fit to the SLS crystalline potential was determined by using the nonlinear coefficients from the bulk fits. The CPU time saved by factorizing the energy matrix integrals and using a rectangular unit cell is discussed. © 1992 John Wiley & Sons, Inc.
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
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  • 10
    Electronic Resource
    Electronic Resource
    New York, NY : Wiley-Blackwell
    International Journal of Quantum Chemistry 44 (1992), S. 673-686 
    ISSN: 0020-7608
    Keywords: Computational Chemistry and Molecular Modeling ; Atomic, Molecular and Optical Physics
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: We report on the progress in a study to determine the structure of radiation-induced Mn defect complexes in CaF2. The purpose of this study is to correlate the calculated transition energies with those measured experimentally and to determine what effect the inclusion of the external lattice field has on these transitions. Unrestricted open shell Hartree-Fock Self-Consistent Field (UHF) calculations were performed on the unperturbed F-center [Ca4F6Vac]1+ S = 1/2, the unperturbed Mn2+ center [MnF8]6- S = 5/2, S = 3/2 and the Mn2+ perturbed F-center [Mn2Ca2F6Vac]1+ S = 11/2, S = 9/2 defect clusters. A method by which the effects of the external lattice field may be incorporated in the SCF procedure is developed and used in these calculations. Several transition energies are calculated and Mulliken population results for these clusters are presented and discussed. © 1992 John Wiley & Sons, Inc.
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
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