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Total DNA 5-methylcytosine level of rice calli derived from protoplasts is independent of regeneration ability (1993)

Tanaka, Yoshimasa ; Oono, Kiyoharu

Springer

Journal of plant research 106 (1993), S. 351-355

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ISSN: 1618-0860

Keywords: 5-Azacytidine ; 5-Methylcytosine ; Plant regeneration ; Protoclone ; Rice callus

Source: Springer Online Journal Archives 1860-2000

Topics: Biology

Notes: Abstract To study the roles of m5C in the differentiation of rice calli derived from protoplasts (protoclones), the m5C level of the total DNA was analyzed using the32P post-labeling method. The level of m5C in regenerable and nonregenerable protoclones was similar, as was in calli and leaves of plants regenerated from the same protoclones. Treatment with 0.5 mM 5-azacytidine caused significant reduction of the level of m5C and of the regeneration frequency of callus. Significantly increased m5C levels were observed during prolonged culture.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1007/BF02345980

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