ISSN:
1432-0584
Keywords:
K 562 cells
;
Erythroid differentiation
;
Hemin
;
Iron chelators
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Summary Erythroid differentiation of human leukemic K 562 cells is inhibited by the iron chelator desferrioxamine (DF). In addition, desferrioxamine induces an increase of uptake of hemin. When hemin is added to the culture medium, the DF-mediated inhibitory effects on erythroid induction are reversed. Briefly, hemin allows hemoglobin synthesis by K 562 cells induced to erythroid differentiation by 1-β-D-arabinofuranosylcytosine (ara-C) and treated with 12.5 μg/ml DF. In addition, it was found that hemin treatment leads to a reversion of inhibition of K 562 cell proliferation mediated by 50–75 μg/ml DF. This effect of hemin was also detected in other cultured human tumor cell lines (B-lymphoid, erythroleukemic and from breast carcinomas, melanomas and kidney carcinomas).
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00320631
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