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  • 1
    ISSN: 1618-2545
    Keywords: barley grain inoculum ; growth promotion ; soybean ; yield components ; zoysiagrass rhizosphere
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Among 21 rhizosphere fungi tested, eight sterile fungi and oneTrichoderma isolate (GT2-1) from zoysiagrass rhizosphere promoted the overall growth of soybean varieties when grown in the greenhouse. Out of nine effective isolates, GS7-4, GS8-2, GS8-3, GU23-3 (all sterile fungi) and GT2-1 (Trichoderma sp.) promoted plant growth and increased yield of Toyosuzu (variety 1) significantly, while GS8-3, GS10-1, GS10-2 (sterile fungi), and GT2-1 significantly caused plant growth promotion and yield increase of Kitamusume (variety 2). Among these efficient isolates, GS8-3 and GS10-2 induced considerable and consistent increases in length, biomass and yield of plants of varieties 1 and 2, respectively. In the field, however, only GS8-3 and GU23-3 among seven selected isolates, induced consistent and significant increases in plant growth and yield of varieties 1 and 2, while the ability of other isolates decreased. The plant growth promotion by these isolates in the field followed a similar trend to that in the greenhouse, but the effect was less marked. Some isolates which were effective in the greenhouse were less effective in the field. The degree of growth promotion by different isolates depended on the variety of soybean. The nutrient condition of soils used in experiments also seemed to play a vital role, since notable growth promotion by these isolates was observed in nutrient-depleted soil.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1618-2545
    Keywords: Chromista ; internal transcribed spacer ; phylogeny ; Pythium ; 5.8S rDNA
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The sequences of ITS regions in 30 species and two groups of the genusPythium were resolved. In the phylogenetic trees, the species were generally divided into two clusters, referred to here as the F and S groups. The species in the two groups correspond in terms of their sporangial morphology, with the F group being filamentous/lobulate and the S group being spherical. Genetic divergence within the F group was lower than that within the S group. Other morphological characteristics such as oogonial structure and sexual nature appeared to be unrelated to the groupings in these trees. An alignment analysis revealed common sequences to all the species and arrangements specific to each F or S group. It was found that the ITS region was a good target in designing species-specific primers for the identification and detection ofPythium species. In the tree based on 5.8S rDNA sequences, oomycetes are distantly related to other fungi but separated from algae in Chromista.
    Type of Medium: Electronic Resource
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  • 3
    ISSN: 1573-8469
    Keywords: turfgrass ; binucleate ; RFLP ; ITS
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Binucleate Rhizoctonia anastomosis group (AG) D is the cause of rhizoctonia-patch and elephant-footprint diseases of zoysiagrass, and winter-patch disease of bentgrass. Rhizoctonia AG-D is also known as the causal pathogen of other diseases such as sharp-eye-spot of cereals, foot-rot of cereals and winter-stem-rot of mat rush. Isolates of AG-D have been divided into the two subgroups AG-D (I) and AG-D (II), based on the results of cultural characteristics and pathogenicity tests. Isolates obtained from zoysiagrass exhibiting symptoms of rhizoctonia-patch disease, from bentgrass with winter-patch disease, from wheat with foot-rot disease, and from mat rush with winter-stem-rot disease were reported to belong to subgroup AG-D (I). On the other hand, isolates obtained from zoysiagrass with elephant-footprint disease were assigned to subgroup AG-D (II). To confirm the existence of these two subgroups in AG-D, the genetic structure of AG-D isolates from turfgrass and other crops was compared. RFLP analysis of the ITS region from rDNA after digestion with the restriction enzymes EcoRI, HaeIII, HhaI, HinfI, and MboI separated AG-D isolates into two groups corresponding to AG-D (I) and AG-D (II). Furthermore, other AGs except AG-Q (AGs-A, Ba, Bb, C, E, F, G, I, K, L, O, P, and R. solani AG1-IC) did not have the same patterns that were seen for the two AG-D subgroups. AG-Q isolates from bentgrass showed the same patterns as AG-D (I). The results of the RAPD analysis also revealed the existence of two groups that corresponded to AG-D (I) and AG-D (II). These analyses revealed that Rhizoctonia AG-D isolates from turfgrass could be divided into two subgroups consistent with those based on cultural characteristics and pathogenicity. In addition, isolates of foot-rot disease of wheat and isolates of winter-stem-rot disease of mat rush whose cultural characteristics were the same as those of AG-D (I) also showed similar RFLP and RAPD patterns to those of AG-D (I) isolates from turfgrass.
    Type of Medium: Electronic Resource
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