ISSN:
1432-1017
Schlagwort(e):
Rhodopsin
;
Light-scattering
;
Flash-photometry
;
Disc membrane
;
Rod outer segments
Quelle:
Springer Online Journal Archives 1860-2000
Thema:
Biologie
,
Physik
Notizen:
Abstract The axial and radial shrinkage of bovine rod outer segments, monitored by near-infrared scattering changes (P-signal), is investigated in dependence on the intensity of the activating flash. Suspensions of axially oriented and randomly oriented rod outer segments were measured. In the latter case, axial and radial effects are superimposed to another. The following results are obtained: 1. The axial signal (P a, Τ≈10 ms) and the radial signal (P r, Τ=40–100 ms), simultaneously measured on axially oriented rod outer segments, are similarly saturated with a half-saturation at a rhodopsin turnover of 3.5%. 2. For the saturation of the signal amplitude, measured on randomly oriented rod outer segments, a good fit is obtained by: $$\begin{gathered} P\left( \varrho \right) \sim 1 - e\beta \varrho , \hfill \\ \varrho : relative rhodopsin turnover by the flash; \hfill \\ \beta is found in the range 23 \leqslant \beta \leqslant 27 in all measurements \hfill \\ \end{gathered} $$ 3. The kinetics of the signal, also measured on the isotropic sample, depends on the rhodopsin turnover, the apparent time constant becoming faster with increasing turnover. The distortion of the signal cannot be fitted by a sum of exponentials with a fixed set of time constants. The signals from the isotropic sample are fitted by a phenomenological model. It introduces three first order processes concatenated in series; the first step is assumed as a rhodopsin transition inducing the two further processes. The distortion of the signals with increasingϱ is then described assuming aϱ-dependent quenching of this induction, according to the measured amplitude saturation. The time constants remain thereby unchanged. The fit yields the values ln 2/k=4, 11, and 45 ms with mean square deviations of 20%.
Materialart:
Digitale Medien
URL:
http://dx.doi.org/10.1007/BF01047108
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