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  • 74.25.Ha  (1)
  • 74.62.Bf  (1)
  • Carbohydrates  (1)
  • Hemoglobin variant  (1)
  • Physics  (1)
  • 1
    Electronic Resource
    Electronic Resource
    Rapid detection of hemoglobin variants by mutagenically separate polymerase chain reaction (MS-PCR) (1995)
    Springer
    Annals of hematology 71 (1995), S. 97-100 
    Details
    ISSN: 1432-0584
    Keywords: Hemoglobin variant ; MS-PCR ; Allele-specific primer
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract The detection of molecular defects of hemoglobin variants using mutagenically separated polymerase chain reaction (MS-PCR) was applied in this study. Using different lengths of allele-specific mutagenic primers, normal and mutant alleles of hemoglobin genes were amplified in the same reaction tube. Subsequent gel electrophoresis showed at least one of the two allelic products at the same loci or at least two of the several allelic products at different loci. We employed MS-PCR to test the following hemoglobin variants: Hb Constant Spring (Hb CS), Hb E, Hb G-Taichung, Hb J-Meinung, and Hb Kaohsiung. The results were the same as those obtained by amplified created reaction sites (ACRS) or direct sequencing. We conclude that the MS-PCR provides a rapid and simple alternative to other techniques for mutation detection in hemoglobin variants. Moreover, the principle can be extended to other genetic diseases.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01699253
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    High-performance liquid chromatography of carbohydrates in enzymic hydrolysate of waste paper (1992)
    Springer
    Chromatographia 34 (1992), S. 534-536 
    Details
    ISSN: 1612-1112
    Keywords: Column liquid chromatography ; Carbohydrates ; Enzymic hydrolysate ; Waste paper
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Summary Waste paper was pretreated with 5M sulfuric acid at 28°C to give a gel-like solution which was then hydrolyzed with cellulase, purified fromTrichoderma viride, to form carbohydrates. The determination of the carbohydrates was accomplished using an aminopropylbonded silica HPLC column and an RI detector. The detection limits were 20 ppm; xylose, glucose, and cellobiose were the major components. Salts from the buffer solution may affect the activity of cellulase. The analysis of carbohydrates can be performed under acid conditions. HPLC analysis of carbohydrates from enzymatic hydrolysis is reliable and successful.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02290248
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Paramagnetic meissner effect in conventional Nb superconductors (1996)
    Springer
    Journal of low temperature physics 105 (1996), S. 509-514 
    Details
    ISSN: 1573-7357
    Keywords: 74.60.Ec ; 74.25.Ha ; 74.60.Ge ; 74.62.Bf
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract The paramagnetic Meissner effect (PME), in which the field-cooled-magnetization (FCM) of superconducting samples is positive below the superconducting transition temperature Tc, has been observed in certain ceramic and single-crystal samples of the high-Tc cuprate superconductors and more recently in disk-shaped Nb samples. Through systematic investigations of the conditions for observing the PME in Nb disks, various surface treatments to the Nb disks were found to change both the zero-field-cooled-magnetization (ZFCM) and the FCM, including the appearance of a positive FCM in samples previously not exhibiting the PME as well as the elimination of the PME through surface abrasion. These results suggest that the PME arises from the field distributions created by the flux pinning associated with microstructural defects on the surface layer of the disk.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00768436
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    Paper (German National Licenses)
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  • 4
    Electronic Resource
    Electronic Resource
    Polymer conformation based on correlated walk model (1980)
    New York : Wiley-Blackwell
    Journal of Polymer Science: Polymer Physics Edition 18 (1980), S. 175-176 
    Details
    ISSN: 0098-1273
    Keywords: Physics ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Physics
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/pol.1980.180180117
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    Paper (German National Licenses)
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