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  • Opaque-2  (3)
  • Sugar beet  (2)
  • A1 locus  (1)
  • 1
    Electronic Resource
    Electronic Resource
    The A1 (anthocyanin-1) locus in Zea mays encodes dihydroquercetin reductase
    Amsterdam : Elsevier
    Plant Science 52 (1987), S. 7-13 
    Details
    ISSN: 0168-9452
    Keywords: A1 locus ; Zea mays ; anthocyanin biosynthesis ; dihydroquercetin reductase
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology
    Type of Medium: Electronic Resource
    URL: http://linkinghub.elsevier.com/retrieve/pii/0168-9452(87)90098-7
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Localization of QTLs for tolerance to Cercospora beticola on sugar beet linkage groups (1999)
    Springer
    Theoretical and applied genetics 99 (1999), S. 829-836 
    Details
    ISSN: 1432-2242
    Keywords: Keywords Resistance ; Sugar beet ; Leaf spot disease (Cercospora beticola) ; Genetic linkage map ; QTLs
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  We present a new linkage map for sugar beet (Beta vulgaris) which has been developed using a population segregating for genetic factors that confer tolerance to the leaf spot fungus (Cercospora beticola), the causal factor of leaf spot disease in sugar beet). In the F2 population studied, a subset of 36 RFLP probes, mapping on eight out of the nine linkage groups of sugar beet, provided the anchor markers to assign chromosomes. A total of 224 markers, including RFLPs, AFLPs, SCARs and microsatellites, were mapped. Estimates of leaf damage in F2 and test-cross families were repeated at different stages of plant development. Each set of data was analysed as such. An average estimate was also considered. QTLs with highly significant LOD scores revealed both by the F2 and test-cross analyses were localized on linkage groups 2, 6 and 9. Linkage groups 4 and 5 gave a clear indication of the presence of a QTL only when F2 data were considered. One highly significant QTL with a LOD of 16.0 was revealed only by the data obtained under conditions of artificial inoculation. This QTL maps at position 90 on chromosome 3.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001220051302
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Linkage relationships between regulatory and structural gene loci involved in zein synthesis in maize (1978)
    Springer
    Theoretical and applied genetics 52 (1978), S. 263-267 
    Details
    ISSN: 1432-2242
    Keywords: Zein synthesis ; Gene regulation ; Opaque-2 ; linkage relationships
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The synthesis of at least 15 zein polypeptides is under the control of regulatory gene loci. One of these, Opaque-2 (chromosome 7, position 16) strongly reduces the zein accumulation without modifying the zein molecular components. The linkage relationship between the regulatory gene 02 and the 5 structural loci (Zp1, Zp2, Zp3, Zp6, Zp12) segregating with sample Mendelian ratios have been studied. Zp1, Zp2, Zp3 are closely linked to each other; moreover this gene cluster is located on chromosome 7 at 5.5 cM from the Opaque-2 locus. The structural loci Zp6 and Zp12 are not linked with each other, with the 02 locus or with Zp1, Zp2, Zp3. From our data it follows that the zein structural genes are located in at least three positions on the maize genome. The scattering in the genome of the genes controlled by the Opaque-2 locus suggests a transacting role for this regulatory element.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00303503
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    Paper (German National Licenses)
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  • 4
    Electronic Resource
    Electronic Resource
    An extended map of the sugar beet genome containing RFLP and RAPD loci (1995)
    Springer
    Theoretical and applied genetics 90 (1995), S. 189-193 
    Details
    ISSN: 1432-2242
    Keywords: Sugar beet ; Beta vulgaris ; RFLP ; RAPD
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract An updated map of sugar beet (Beta vulgaris L. ssp. vulgaris var ‘altissima Doell’) is presented. In this genetic map we have combined 248 RFLP and 50 RAPD loci. Including the loci for rhizomania resistance Rr1, hypocotyl colour R and the locus controlling the monogerm character M, 301 loci have now been mapped to the nine linkage groups covering 815 cM. In addition, the karyotype of some of the Beta vulgaris chromosomes has been correlated with existing RFLP and RAPD linkage maps.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00222201
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    Paper (German National Licenses)
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  • 5
    Electronic Resource
    Electronic Resource
    The role of multiple binding sites in the activation of zein gene expression byOpaque-2 (1996)
    Springer
    Molecular genetics and genomics 252 (1996), S. 723-732 
    Details
    ISSN: 1617-4623
    Keywords: Opaque-2 ; 22 kD α-zeins ; Endosperm ; Transient gene expression ; Particle bombardment
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Opaque-2 (O2) encodes a transcriptional activator of the basic domain-leucine zipper (bZIP) class, which controls the expression level in maize endosperm of the 22 kD α-zeins and a number of non-storage proteins. The interaction of the O2 protein at three clustered binding sites on an isolated 22 kD zein gene promoter has been investigated. O2 is shown to transactivate transcription from these sites in tobacco mesophyll protoplasts as well as in maize endosperm cells transformed by particle bombardment. The binding sites have been mutated by base exchanges, singly or in different combinations, to determine their contribution to transactivation in vivo in both the leaf protoplast and the maize endosperm system. The effect of these mutations on binding of O2 in vitro was determined by electrophoretic mobility shift assays (EMSA), using O2 protein expressed inE. coli. Two of the sites seemed to be equally effective in responding to Opaque-2 in vivo in both cell types, although one of them does not contain an ACGT core sequence, and has a lower affinity for O2 in vitro than the ACGT-containing binding site. A third site, which has the lowest affinity of all three, confers no detectable O2-dependent promoter activation alone, but significantly increases activation in combination with either one of the other sites. Hence, weaker O2 binding sites can still mediate major O2-dependent effects when present in target promoters in vivo.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02173979
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    Paper (German National Licenses)
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  • 6
    Electronic Resource
    Electronic Resource
    Molecular analysis of theBg-rbg transposable element system ofZea mays L. (1991)
    Springer
    Molecular genetics and genomics 227 (1991), S. 91-96 
    Details
    ISSN: 1617-4623
    Keywords: Zea mays ; Transposable elements ; Opaque-2 ; Waxy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The two components of theBg-rbg transposable element system of maize have been cloned. TheBg element, isolated from the mutable allelewx-m32 :: Bg is inserted in the intron of theWaxy (Wx) gene between exons 12 and 13. The length of the element is of 4869 bp.Bg has 5 by terminal inverted repeats, and generates upon insertion an 8 by direct duplication of the target sequence. Both ends of theBg element contain a 76 by direct repeat adjacent to the terminal inverted repeats. The hexamer motif TATCGkC G is here repeated several times in direct or inverse orientation. Therbg element was isolated from the mutable alleleo2m(r) where it is located in the promoter region of theOpaque-2 (O2) gene.rbg is approximately 4.5 kb in length, has terminal inverted repeats identical to those of theBg element, and is also flanked by an 8 by direct duplication at the target site. LikeBg, rbg carries the 76 by direct repeats. Restriction enzyme analysis reveals that, compared toBg, the receptor element is distinguishable by small deletion and insertion events. Sequence data indicate that not more than 75% homology exists at the DNA level between therbg element and the autonomousBg element.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00260712
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    Paper (German National Licenses)
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