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  • 1
    Electronic Resource
    Electronic Resource
    Acetylcholine dose-response relation and the effect of cesium ions in the rat adrenal chromaffin cell under voltage clamp (1987)
    Springer
    Pflügers Archiv 408 (1987), S. 401-407 
    Details
    ISSN: 1432-2013
    Keywords: ACh-induced current ; Adrenal chromaffin cell ; ACh dose-response curve ; Channel block by CS ions ; Whole cell voltage clamp
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Acetylcholine(ACh)-induced inward current was examined in the rat adrenal chromaffin cell in culture. Cells were voltage clamped with one electrode using the giga-seal technique. The ACh dose-response relationship was determined by applying various concentrations of ACh with the “puffer” method. Although there were several technical difficulties, we found tentative figures as 135 to 480 μM for the dissociation constant and 1.2 to 1.4 for the Hill coefficient. External Cs ions had a depressing effect on the ACh reponse. Cs ions may slowly block ACh receptor channels from outside at the resting state. The ACh response in normal saline recorded with the Cs-filled electrode reversed the polarity at −7.1 mV. When the amplitude of ACh-induced current was plotted against holding membrane potential the curve showed strong rectification at the region more negative than the reversal potential. Between +10 and +30 mV there was a region of a negative slope. We discussed possible mechanisms for this characteristic I–V curve.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00581136
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Isolation of clones from T24 cells and test of their sensitivity to adriamycin (1988)
    Springer
    Research in experimental medicine 188 (1988), S. 239-246 
    Details
    ISSN: 1433-8580
    Keywords: T24 ; Heterogeneity ; Single cell clone ; Adriamycin ; Chemosensitivity
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Two single cell clones, CL3 and CL7, were developed from T24 cells. CL3 had abundant cytoplasmic granules, while CL7 did not have as much. CL3 and CL7 exhibited a modal number of chromosomes at 69 and 74, respectively. CL7 had a higher growth rate than CL3, as indicated by in vitro cell growth, plating efficiency, and growth of the tumors produced in nude mice. CL3 was slightly susceptible to adriamycin toxicity as compared to CL7.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01852272
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
    Fulltext
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