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  • 1
    Electronic Resource
    Electronic Resource
    A detailed linkage map around an apple scab resistance gene demonstrates that two disease resistance classes both carry the Vf gene (1996)
    Springer
    Theoretical and applied genetics 93 (1996), S. 485-493 
    Details
    ISSN: 1432-2242
    Keywords: Key wordsMalus×domestica ; Venturia inaequalis ; Malus floribunda ; Genetic map ; Disease resistance
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A detailed genetic map has been constructed in apple (Malus×domestica Borkh.) in the region of the V f gene. This gene confers resistance to the apple scab fungus Venturia inaequalis (Cooke) Wint‥ Linkage data on four RAPD (random amplified polymorphic DNA) markers and the isoenzyme marker PGM-1, previously reported to be linked to the V f gene, are integrated using two populations segregating for resistance to apple scab. Two new RAPD markers linked to V f (identified by bulked segregant analysis) and a third marker previously reported as being present in several cultivars containing V f are also placed on the map. The map around V f now contains eight genetic markers spread over approximately 28 cM, with markers on both sides of the resistance gene. The study indicates that RAPD markers in the region of crab apple DNA introgressed with resistance are often transportable between apple clones carrying resistance from the same source. Analysis of co-segregation of the resistance classes 3A (weakly resistant) and 3B (weakly susceptible) with the linked set of genetic markers demonstrates that progeny of both classes carry the resistance gene.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001220050306
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    Paper (German National Licenses)
    Fulltext
  • 2
    Electronic Resource
    Electronic Resource
    Microsatellites in Malus X domestica (apple): abundance, polymorphism and cultivar identification (1997)
    Springer
    Theoretical and applied genetics 94 (1997), S. 249-254 
    Details
    ISSN: 1432-2242
    Keywords: Key words Microsatellites ; Apple ; Identification
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Screening of an apple genomic library with (GA)15 and (GT)15 probes demonstrated that these repeats are abundant, occurring about every 120 and 190 kb, respectively. Microsatellites isolated from a small insert library enriched for (GA) repeats contained numbers of repeats ranging from 7 to 39. Primers to these microsatellite loci were able to direct the amplification of the repeats in 21 different cultivars. The majority of markers were highly polymorphic, diploid, and showed simple Mendelian inheritance, although about 25% of markers generated complex banding patterns consistent with the amplification of more than one locus. As few as three microsatellite markers were sufficient to differentiate between all 21 cultivars.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001220050407
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
    Fulltext
  • 3
    Electronic Resource
    Electronic Resource
    A detailed linkage map around an apple scab resistance gene demonstrates that two disease resistance classes both carry the V f gene (1996)
    Springer
    Theoretical and applied genetics 93 (1996), S. 485-493 
    Details
    ISSN: 1432-2242
    Keywords: Malus x domestica ; Venturia inaequalis ; Malus floribunda ; Genetic map ; Disease resistance
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A detailed genetic map has been constructed in apple (Malus x domestica Borkh.) in the region of the v f gene. This gene confers resistance to the apple scab fungus Venturia inaequalis (Cooke) Wint. Linkage data on four RAPD (random amplified polymorphic DNA) markers and the isoenzyme marker PGM-1, previously reported to be linked to the v f gene, are integrated using two populations segregating for resistance to apple scab. Two new RAPD markers linked to v f (identified by bulked segregant analysis) and a third marker previously reported as being present in several cultivars containing v f are also placed on the map. The map around v f now contains eight genetic markers spread over approximately 28 cM, with markers on both sides of the resistance gene. The study indicates that RAPD markers in the region of crab apple DNA introgressed with resistance are often transportable between apple clones carrying resistance from the same source. Analysis of co-segregation of the resistance classes 3A (weakly resistant) and 3B (weakly susceptible) with the linked set of genetic markers demonstrates that progeny of both classes carry the resistance gene.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00417939
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
    Fulltext
  • 4
    Electronic Resource
    Electronic Resource
    Unique distribution of the anion exchange protein in the sea lamprey, Petromyzon marinus (2000)
    Springer
    Journal of comparative physiology 170 (2000), S. 497-504 
    Details
    ISSN: 1432-136X
    Keywords: Key words Anion exchange ; AE1 ; Red blood cells ; Lamprey ; Respiration ; AbbreviationsA carbonic anhydrase activity ; CA carbonic anhydrase ; DIDS 4,4′ diisothiocyanatostilbene-2,2′ disulphonic acid ; GTC-AP guanidium isothiocyanate-acid phenol ; IgGs type G immunoglobins ; P red blood cell HCO−3 permeability ; PCR polymerase chain reaction ; rbc(s) red blood cell(s)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract The purpose of the present study was to examine the distribution of the anion exchange protein in the sea lamprey. Southern blots showed that genomic DNA of juvenile lampreys possesses several regions that are similar to segments of the AE gene from other vertebrates. However, physiological experiments examining rapid anion fluxes across the red blood cell (rbc) membrane and molecular experiments examining mRNA transcript levels both indicated that the anion exchange protein is absent in sea lamprey rbcs. In contrast, lamprey kidney, skeletal muscle, liver and heart tissue all appeared to possess mRNA transcripts for an AE protein. Further evidence for the presence of an AE protein in kidney tissue was obtained from Western blots. In order to evaluate the impact of the apparent rbc anion exchange limitations, the bicarbonate permeability of lamprey rbcs was also evaluated using mass spectrometry. The bicarbonate permeability of the lamprey rbc membrane was an order of magnitude lower than that of trout rbcs. Taken together, these results indicate that the gene for the AE protein is indeed present in lampreys, but it is not expressed in the rbc. Moreover, the process of CO2 transport in lamprey probably does not involve bicarbonate transport across the rbc membrane.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s003600000127
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    Paper (German National Licenses)
    Fulltext
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