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  • In situ hybridization  (2)
  • AML blast cells  (1)
  • Aplastic anemia  (1)
  • Crossreacting antibodies  (1)
  • 1
    Electronic Resource
    Electronic Resource
    Auer-rod positive myelocytic leukemia cells in diffusion chambers: Differentiation along the eosinophilic pathway (1982)
    Springer
    Annals of hematology 45 (1982), S. 323-328 
    Details
    ISSN: 1432-0584
    Keywords: AML blast cells ; Eosinophilic differentiation ; Diffusion chambers
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Auer-rod positive acute myelocytic leukemia (AML) blasts from a 33-year-old patient were cultured in diffusion chambers (DC) in order to test their differentiation potential. The cells were labeled with anti-human granulocyte antiserum known to be negative for eosinophils, and evaluated using the unlabeled peroxidase-antiperoxidase (PAP) method. Parallel to a decline in the number of leukemic blasts there was an increase of up to 86% in the number of granulocytic cells belonging to the eosinophilic series. Auer-rod bodies were found in the eosinophilic cells even after 20 days in culture. Staining with anti-granulocyte antiserum failed to demonstrate positive cells at any time during DC culture. Based on the negative reaction with the anti-granulocytic antibodies already at an early stage of development evidence is provided for the existence of a progenitor cell exclusively committed to the eosinophilic pathway.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00319526
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Identification of the messenger (m) RNA coding for the constant fragment (cμ) of the heavy chain with cloned DNA in single cells by in situ hybridization (1983)
    Springer
    Annals of hematology 46 (1983), S. 107-110 
    Details
    ISSN: 1432-0584
    Keywords: Immunoglobulin expression ; μ-mRNA ; In situ hybridization
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Cells from a normal donor, a B-CLL and a T-ALL were labeled with an FITC conjugated rabbit antihuman IgM and hybridized with a rhodamineconjugated cloned DNA coding for the cμ segment. The cells were measured simultaneously by immunofluorimetry for their surface content of IgM and their amount of m-RNA hybridizing with the cloned DNA. Thus it was possible to compare the transcription of the gene coding for the immunoglobulin μ chain and the expression of IgM in individual cells. It could be shown that besides the immunoglobulin μ containing B-cells, which were expected to contain the respective m-RNA, cells were found which did not express IgM but which were still positive for the m-RNA.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00320667
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Quantitation of globin mRNA in individual human erythroblasts by in situ hybridization (1981)
    Springer
    Annals of hematology 43 (1981), S. 79-83 
    Details
    ISSN: 1432-0584
    Keywords: Erythropoiesis ; Aplastic anemia ; Globin mRNA ; In situ hybridization
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary In situ hybridization of globin mRNA was performed in four human bone marrows using a 3H-cDNA derived from rat globin messenger. Erythroblasts were classified according to nuclear area as well as to morphological appearance after Giemsa staining. Grains over individual erythroblasts and reticulocytes were counted by incident light microphotometry. Reticulocytes from phenyl hydrazine-treated rats served as internal standards in order to allow a comparison of different experiments. The preliminary results from one normal bone marrow, one of stress erythropoiesis and two of aplastic anemia revealed the major content of globin mRNA in the stages of basophilic erythroblasts or proerythroblasts. In all cases there was a tendency of decreasing globin mRNA content towards orthochromatic erythroblasts, and even more so, towards reticulocytes. The results show some differences between normal and diseased erythropoiesis which are under study on a larger scale now in order to ascertain their significance.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00320464
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    Paper (German National Licenses)
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  • 4
    Electronic Resource
    Electronic Resource
    Die Zellspezifität heterologer, sogenannter antilymphocytärer Antiseren vom Kaninchen (1968)
    Springer
    Clinical and experimental medicine 147 (1968), S. 44-59 
    Details
    ISSN: 1591-9528
    Keywords: Antilymphocyte serum ; Crossreacting antibodies ; Antilymphocytenserum ; Kreuzreagierende Antikörper
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Zusammenfassung Im heterologen Antimäuse- bzw. Antirattenlymphocytenserum vom Kaninchen ließen sich die folgenden Antikörpertypen unterscheiden: 1. Anti-Erythrocyten/Leukocyten/Thrombocyten/Leber/Nieren-Antikörper; 2. Anti-Leukocyten /Thrombocyten/Leber/Nieren-Antikörper; 3. Antileukocytenantikörper; 4. Antierythrocytenantikörper; 5. Antiserumproteinantikörper. Während antierythrocytäre und antileukocytäre Antikörper sowohl in der 7 S als auch in der 19 S-Globulin-Fraktion auftraten, wurden Antikörper, die gegen ein den Erythrocyten und Leukocyten gemeinsames Antigen gerichtet sind, nur in der 7 S-Fraktion nachgewiesen. Die Bedeutung des letzteren Antikörpertyps, insbesondere für die Absorption der Erythrocytenagglutinine, wird diskutiert. Die gegen Granulocyten gerichtete Aktivität sogenannter antilymphocytärer Antikörper wird durch Komplementbindungsteste, Leukocytenkurven und den Nachweis extramedullärer sowie extrasplenaler Blutbildungszentren in der Leber wahrscheinlich gemacht. Neben dem Fehlen der Gruppenspezifität wird gezeigt, daß die Kreuzreaktion zwischen Blutzellen von Ratte und Maus auf einen Teil der Antikörper beschränkt ist. Die gegen Organextrakte auftretenden Präzipitationen des Antilymphocytenserums werden als organunspezifisch interpretiert.
    Notes: Summary The following types of antibodies were differentiated in heterologous rabbit antisera against mouse or rat lymphocytes: 1. Anti-erythrocyte/leucocyte/ thrombocyte/liver/kidney-antibody; 2. Anti-leucocyte/thrombocyte/liver/kidney-antibody; 3. Anti-leucocyte-antibody; 4. Anti-erythrocyte-antibody; 5. Anti-serum-protein-antibody. While anti-erythrocyte and anti-leucocyte antibodies were found in the 7 S-as well as in the 19 S-Globulin-Fraction, antibodies against an antigen common to red cells and leucocytes were only demonstrated in the 7 S-fraction. The practical implications with the latter antibody what regards the absorption of red cell agglutinins were considered. Antibody activity against granulocytes was demonstrated by complementfixing tests, granulocyte count and extramedullary haemopoesis in the liver. The antibodies lack blood group specificity. Cross-reactivity between blood cells of rat and mouse does not involve all of the antibodies. The precipitation lines of the antibodies against organ extracts were not interpreted as organ specific.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02044590
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