ISSN:
1573-4986
Keywords:
glycosylation
;
Na+,K+-ATPase
;
oligomannose
;
polylactosamine
;
tissue-specific
;
2-AB, 2-aminobenzamide
;
AMOG, adhesion molecule on glia
;
ATP, adenosine triphosphate
;
BSA, bovine serum albumin
;
CAM, calcium/calmodulin-dependent protein kinase
;
CNS, central nervous system
;
DSA, Datura stramonium agglutinin (lactosamine and sialic acid recognition)
;
EDTA, ethylenediaminetetra-acetic acid
;
Gal, galactose
;
Glc-NAc, N-acetyl-D-glucosamine
;
GNA, Galanthus nivalis agglutinin (oligomannosidic recognition)
;
GnT, N-acetylglucosaminyltransferase
;
GU, glucose unit
;
HPLC, high performance liquid chromatography
;
MALDI, matrix-assisted laser desorption/ionisation
;
(Man)5–9, oligomannose-type glycan series with 5–9 manose residues
;
Man1, Manβ1-4GlcNAcβ1-4 GlcNAc
;
Man3, trimannose-chitobiose core
;
Man3F, trimannose-chitobiose core with fucose on reducing terminal GlcNAc
;
MS, mass spectrometry
;
NP normal phase
;
PBS, phosphate buffered saline
;
PNGase F, protein-N-glycosidase F
;
PVDF, poly(vinylidene difluoride)
;
SDS-PAGE, sodium dodecyl sulphate polyacrylamine gel electrophoresis
;
TOF, time-of-flight
;
Tris, 2-amino-2-hydroxymethylpropane-1,3 diol
;
WAX, weak anion exchange
Source:
Springer Online Journal Archives 1860-2000
Topics:
Chemistry and Pharmacology
Notes:
Abstract The organ-specific nature of the glycosylation of Na+,K+-ATPase-enriched preparations from kidney and brain tissues has earlier been indicated by the use of lectin-staining techniques. Na+,K+-ATPase is ubiquitous and abundant, and subject to upregulation during cell-division and in certain pathological conditions. Lectins specific for the different carbohydrates displayed by the Na+,K+-ATPases may, therefore, be useful carriers/mediators in tissue-specific targeting. N-linked oligosaccharides purified from Na+,K+-ATPase-enriched preparations from rat brain and kidney were consequently characterised in detail in this study using weak anion exchange and normal phase HPLC (combined with serial glycosidase digestions) and matrix-assisted laser desorption/ionisation mass spectrometry. The oligomannose series of glycans were most abundant in the brain tissue preparation and this contrasted with the renal-associated oligosaccharides that were dominated by families of tetra-antennary glycans (with/without a core fucose) with up to four lactosaminylglycan residues in either branched or linear formation.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1023/A:1007078511110
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