Library

X
feed icon rss

Your email was sent successfully. Check your inbox.

An error occurred while sending the email. Please try again.

Proceed reservation?

Export
  • 1
    Electronic Resource
    Electronic Resource
    Oligosaccharide sequencing technology (1997)
    [s.l.] : Macmillan Magazines Ltd.
    Nature 388 (1997), S. 205-207 
    Details
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] Recent advances allow any well-founded laboratory to carry out rapid and straightforward characterization of subpicomolar levels of N-glycans released from one to five microgram quantities of ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/388205a0
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 2
    Electronic Resource
    Electronic Resource
    Matrix-assisted laser desorption mass spectrometry on a magnetic sector instrument fitted with an array detector (1994)
    New York, NY : Wiley-Blackwell
    Rapid Communications in Mass Spectrometry 8 (1994), S. 585-589 
    Details
    ISSN: 0951-4198
    Keywords: Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Physics
    Notes: Matrix-assisted laser desorption/ionization (MALDI) has been carried out on a high performance double-focusing mass spectrometer fitted with an integrating focal plane array detector. High sensitivity (low femtomole detection) was obtained with peptides such as renin substrate. Underivatized complex oligosaccharides were also detectable at the 200 fmol level. The advantages of the array detector for improved resolution (5000 full width at half maximum) and the benefits in complex oligosaccharide and glycolipid analyses relative to MALDI time-of-flight (TOF) systems are shown. This improved resolution enabled ambiguities from the TOF data to be interpreted.
    Additional Material: 11 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/rcm.1290080803
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 3
    Electronic Resource
    Electronic Resource
    Comparison of fragmentation modes for the structural determination of complex oligosaccharides ionized by matrix-assisted laser desorption/ionization mass spectrometryPresented at the 21st Annual Meeting of the British Mass Spectrometry Society, UMIST, Manchester, 3-6 September 1995. (1995)
    New York, NY : Wiley-Blackwell
    Rapid Communications in Mass Spectrometry 9 (1995), S. 1556-1561 
    Details
    ISSN: 0951-4198
    Keywords: Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Physics
    Notes: Fragment ions from underivatized N-linked oligosaccharides ionized by matrix-assisted laser desorption/ionization mass spectrometry were obtained by spontaneous fragmentation on a magnetic sector mass spectrometer, by post-source decay (PSD) on a reflectron time-of-flight (TOF) instrument and by collision-induced dissociation on a magnetic sector instrument fitted with an orthogonal-TOF analyser. Spontaneous fragmentation on the magnetic sector instrument produced ions mainly by glycosidic cleavage together with two abundant ions formed by cross-ring cleavage of the reducing-terminal residue. The PSD spectra were similar, the majority of ions being formed by glycosidic cleavage. Internal fragment ions were abundant. High-energy collision-induced dissociation spectra, recorded with the orthogonal-TOF analyser, differed considerably from the other types of spectra, particularly in the appearance of major fragment ions produced by cross-ring cleavages of most of the constituent monosaccharide residues. These ions allowed much sequence and branching information to be obtained from the oligosaccharide.
    Additional Material: 6 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/rcm.1290091517
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 4
    Electronic Resource
    Electronic Resource
    The role of mass spectrometry in glycobiology (1992)
    Springer
    Glycoconjugate journal 9 (1992), S. 1-12 
    Details
    ISSN: 1573-4986
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00731169
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 5
    Electronic Resource
    Electronic Resource
    Perspectives in the glycosciences – matrix-assisted laser desorption/ionization (MALDI) mass spectrometry of carbohydrates (1998)
    Springer
    Glycoconjugate journal 15 (1998), S. 333-338 
    Details
    ISSN: 1573-4986
    Keywords: mass spectrometry ; MALDI ; fragmentation ; post-source decay
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Abstract The technique of matrix-assisted laser desorption/ionization (MALDI) mass spectrometry is reviewed with emphasis on the best type of matrix to use with carbohydrates, ways to obtain fragmentation and use of the technique for carbohydrate sequencing. © 1998 Rapid Science Ltd
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1006913616252
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 6
    Electronic Resource
    Electronic Resource
    Characterisation of tissue-specific oligosaccharides from rat brain and kidney membrane preparations enriched in Na+,K+-ATPase (1999)
    Springer
    Glycoconjugate journal 16 (1999), S. 437-456 
    Details
    ISSN: 1573-4986
    Keywords: glycosylation ; Na+,K+-ATPase ; oligomannose ; polylactosamine ; tissue-specific ; 2-AB, 2-aminobenzamide ; AMOG, adhesion molecule on glia ; ATP, adenosine triphosphate ; BSA, bovine serum albumin ; CAM, calcium/calmodulin-dependent protein kinase ; CNS, central nervous system ; DSA, Datura stramonium agglutinin (lactosamine and sialic acid recognition) ; EDTA, ethylenediaminetetra-acetic acid ; Gal, galactose ; Glc-NAc, N-acetyl-D-glucosamine ; GNA, Galanthus nivalis agglutinin (oligomannosidic recognition) ; GnT, N-acetylglucosaminyltransferase ; GU, glucose unit ; HPLC, high performance liquid chromatography ; MALDI, matrix-assisted laser desorption/ionisation ; (Man)5–9, oligomannose-type glycan series with 5–9 manose residues ; Man1, Manβ1-4GlcNAcβ1-4 GlcNAc ; Man3, trimannose-chitobiose core ; Man3F, trimannose-chitobiose core with fucose on reducing terminal GlcNAc ; MS, mass spectrometry ; NP normal phase ; PBS, phosphate buffered saline ; PNGase F, protein-N-glycosidase F ; PVDF, poly(vinylidene difluoride) ; SDS-PAGE, sodium dodecyl sulphate polyacrylamine gel electrophoresis ; TOF, time-of-flight ; Tris, 2-amino-2-hydroxymethylpropane-1,3 diol ; WAX, weak anion exchange
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Abstract The organ-specific nature of the glycosylation of Na+,K+-ATPase-enriched preparations from kidney and brain tissues has earlier been indicated by the use of lectin-staining techniques. Na+,K+-ATPase is ubiquitous and abundant, and subject to upregulation during cell-division and in certain pathological conditions. Lectins specific for the different carbohydrates displayed by the Na+,K+-ATPases may, therefore, be useful carriers/mediators in tissue-specific targeting. N-linked oligosaccharides purified from Na+,K+-ATPase-enriched preparations from rat brain and kidney were consequently characterised in detail in this study using weak anion exchange and normal phase HPLC (combined with serial glycosidase digestions) and matrix-assisted laser desorption/ionisation mass spectrometry. The oligomannose series of glycans were most abundant in the brain tissue preparation and this contrasted with the renal-associated oligosaccharides that were dominated by families of tetra-antennary glycans (with/without a core fucose) with up to four lactosaminylglycan residues in either branched or linear formation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1007078511110
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 7
    Electronic Resource
    Electronic Resource
    Structural determination of N-linked carbohydrates by matrix-assisted laser desorption/ionization-mass spectrometry following enzymatic release within sodium dodecyl sulphate-polyacrylamide electrophoresis gels: Application to species-specific glycosylation of α1-acid glycoprotein (1998)
    Weinheim : Wiley-Blackwell
    Electrophoresis 19 (1998), S. 1950-1959 
    Details
    ISSN: 0173-0835
    Keywords: Carbohydrates ; Matrix assisted laser desorption ; ionization-mass spectrometry ; Sodium dodecyl sulfate - polyacrylamide gel electrophoresis ; Peptide N-glycosidase P ; α1-Acid glycoprotein ; Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: This paper describes a sensitive method for analysis of N-linked carbohydrates released enzymatically from within the gel following separation of glycoproteins (50-100 pmols) by sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE). The separated bands containing the glycoproteins were cut from the gel, destained, reduced and alkylated. N-linked glycans were then released by in-gel incubation with peptide N-glycosidase-F (PNGase-F) and extracted with water and acetonitrile. Sialic acid-containing glycans were converted into methyl esters by reaction with methyl iodide, salts and reagents were removed by passage through a mixed-bed column of ion-exchange resins and the glycans were examined by matrix-assisted laser desorption/ionization (MALDI)-mass spectrometry. Structural determination of the released glycans was performed by exoglycosidase digestion. Following glycan release and extraction, the protein could be digested within the gel with trypsin, and the masses of the tryptic peptides could be compared with those generated from a sequence database for protein identification. The method is applied to the analysis of N-linked glycans from α1-acid glycoprotein from man, cow, sheep and dog. Major species-specific differences in glycosylation were found. Thus, although all four species used N-acetyl-neuraminic acid, only cow and sheep additionally used N-glycolyl-neuraminic acid. Biantennary glycans were the predominant carbohydrates in cow, sheep and dog but man produced more triantennary glycans and a substantial amount of tetraantennary sugars. Fucosylation was only found in glycans from man and cow and both cow and sheep glycans were found to have β1-3- and well as β1-4-linked galactose residues in the antennae.
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/elps.1150191113
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 8
    Electronic Resource
    Electronic Resource
    Influence of the 6-trimethylsilyl group on the fragmentation of the trimethylsilyl derivatives of some 6-hydroxy- and 3,6-dihydroxy-steroids and related compounds (1979)
    Chichester : Wiley-Blackwell
    Biological Mass Spectrometry 6 (1979), S. 135-143 
    Details
    ISSN: 0306-042X
    Keywords: Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: The 25 eV mass spectra of the trimethylsilyl derivatives of a number of 6-hydroxy and 3,6-dihydroxy steroids together with deuterium and 18O-labeled analogs have been examined to determine the influence of the 6-OTMS group on fragmentation patterns. Ions in the cholestane series at m/z 321 and 403 were the most characteristic ions derived from the 6-OTMS function; their relative abundances, although low in the spectra of 6-OTMS steroids themselves, were considerably elevated when a 3-OTMS or 3-oxo group was present. Similar ions were present in the spectra of androstane and pregnane derivatives. No correlation was found between the abundance of these ions and the stereochemistry at C3, C5, or C6. Fragmentation mechanisms and gas chromatographic data are discussed.
    Additional Material: 4 Tab.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bms.1200060402
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 9
    Electronic Resource
    Electronic Resource
    Determination of N-terminal myristoylation of proteins using a combined gas chromatographic/mass spectrometric assay of derived myristoylglycine: Electron impact-induced fragmentation of acylglycine derivatives (1995)
    Chichester : Wiley-Blackwell
    Biological Mass Spectrometry 30 (1995), S. 900-910 
    Details
    ISSN: 1076-5174
    Keywords: Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Physics
    Notes: A method based on gas chromatography/mass spectrometry is described for the detection of N-terminal myristoylation of proteins. Myristoylglycine, derivatized as its trimethylsily (TMS) ester, gave an electron impact mass spectrum containing abundant molecular and and [M — CH3]+ ions, together with several ions diagnostic of the acyl glycine moiety, namely at m/z 145, 158, 172 and 189. The compositions of these ions and the mechanisms that produced them were investigated by high-resolution mass measurements, deuterium labelling and the preparation of analogous compounds. As these were present in the spectra of all acylglycines examined, they could be used as markers for these compounds. A selected-ion monitoring method for the detection of myristoylglycine was set up using the above ions and was used to confirm the presence of N-terminal myristoylation in three reference peptides. A series of ions produced by radical-induced cleavage of the alkyl chain following TMS group migration and elimination of carbon dioxide gave information on the structure of the chain and could be used to determine the structure of other potential acylglycines such as those with unsaturated acyl chains. Thus the derivatives could be used not only to detect myristoylation of protein, but also to detect and determine the structures of other acyl substituents.
    Additional Material: 6 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jms.1190300616
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 10
    Electronic Resource
    Electronic Resource
    Matrix-assisted laser desorption/ionization mass spectrometry of sphingo- and glycosphingo-lipids (1995)
    Chichester : Wiley-Blackwell
    Biological Mass Spectrometry 30 (1995), S. 1311-1324 
    Details
    ISSN: 1076-5174
    Keywords: Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Physics
    Notes: Sphingo- and glycosphingo-lipids were examined by matrix-assisted laser desorption/ionization mass spectrometry on a time-of-flight (TOF) and a magnetic sector mass spectrometer with 2,5-dihydroxybenzoic acid (2,5-DHB), sinapinic acid, α-cyano-4-hydroxycinnamic acid, 2-(4-hydroxyphenylazo)benzoic acid (HABA) and esculetin as the matrices. Strong signals were produced by all lipids tested, with many of the compounds producing structurally useful fragment ions. Neutral glycosphingolipids gave the best signals in the positive-ion mode with 2,5-DHB, α-cyano-4-hydroxycinnamic acid and esculetin. When 2,5-DHB was used as the matrix, glycosphingolipids that did not contain HexNAc residues tended to fragment between the sugar and ceramide moieties to give ions revealing the saccharide and lipid contents. Other matrices tended not to induce this fragmentation but, instead, produced ions resulting from loss of that acylamide from the ceramide moiety. These ions revealed the distribution of carbon atoms between the acyl and sphingosine chains. Fragmentation of the oligosaccharide chain of glycolipids that did contain HexNAc groups tended to be more random than that seen when HexNAc was absent and provided considerable sequence information. The acidic gangliosides produced broad, unresolved fragment ion peaks on the TOF spectrometer. Fragmentation was the result of both decarboxylation and loss of sialic acids. On the magnetic sector instrument, these fragment ions were sharp and well resolved. Additional fragment ions resulting from glycosidic cleavages provided information about the sequence of sugars in the oligosaccharide chain. Negative-ion signals ([M - H])- from the acidic glycosphingolipids were generally better defined than those in the positive-ion spectra as the result of a reduction in alkali metal adduct and salt formation.
    Additional Material: 19 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jms.1190300916
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
Close ⊗
This website uses cookies and the analysis tool Matomo. More information can be found here...