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  • ATPase  (2)
  • ATPase cytochemistry  (2)
  • Pisum sativum  (2)
  • 1
    Electronic Resource
    Electronic Resource
    Characterization of solute transport in plasma membrane vesicles isolated from cotyledons ofRicinus communis L. (1990)
    Springer
    Planta 182 (1990), S. 532-539 
    Details
    ISSN: 1432-2048
    Keywords: ATPase ; Plasma membrane ; Pyrophosphatase ; Ricinus (solute transport) ; Sucrose transport
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A highly enriched plasma membrane fraction has been isolated from dark-grown cotyledons ofRicinus communis by phase partitioning. This is demonstrated by the properties of the associated ATPase: high vanadate sensitivity, azide and nitrate insensitivity, sharp pH optimum around 6.5, and high specificity for ATP as substrate. The upper plasma membrane fraction also contained a pyrophosphatase activity, normally considered to be located on the tonoplast or Golgi membranes, which showed a specific activity higher than that in the lower phase. Sucrose gradient centrifugation of both microsomal and upper phase fractions showed a comigration of some pyrophosphatase activity with the plasma membrane fraction. Sucrose uptake changes with development inRicinus cotyledons. The ATPase activity in the upper (plasma membrane) phase also varied in a similar way with development, whereas activity in the lower phase showed little change. Pyrophosphatase activity in the upper phase also increased with development but did not show a peak and fall as seen for sucrose uptake and ATPase. The possibility that changes in plasma membrane ATPase may contribute to changes in sucrose uptake capacity and the possible cellular origin and physiological significance of the pyrophosphatase activity are discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02341028
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    A quantitative stereological analysis of the effect of indoleacetic acid on the dictyosomes in pea stem epidermal cells (1985)
    Springer
    Protoplasma 125 (1985), S. 230-234 
    Details
    ISSN: 1615-6102
    Keywords: Dictyosomes ; Indoleacetic acid ; Pisum sativum ; Stereology
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary A quantitative analysis of electron micrographs showed that IAA treatment caused an initial rapid increase in the amount of dictyosome material in pea stem epidermal cells. The increase was detected within 15 minutes of auxin presentation and reached a maximum around 30 minutes. This was followed by a decrease, presumably due to an increased utilization of the organelle. The decrease involved a fall in the amount of dictyosome-derived vesicles and in the actual number of dictyosomes. The results are discussed in relation to similar observations on expanding cells of monocotyledonous plants.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01281242
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Cytochemical localization of ATPase activity in phloem tissues ofRicinus communis (1980)
    Springer
    Protoplasma 104 (1980), S. 55-65 
    Details
    ISSN: 1615-6102
    Keywords: ATPase cytochemistry ; Phloem ; Ricinus communis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Standard lead precipitation procedures have been used to examine the localization of ATPase activity in phloem tissues ofRicinus communis. Reaction product was localized on the plasma membrane of the companion cells associated with sieve elements and of parenchyma cells in phloem tissues from the leaf, petiole, stem and root. ATPase activity was also present on the plasma membrane and dispersed P-protein of sieve elements in petiole, stem and root tissue, but was absent from the plasma membrane of these cells in the leaf minor veins. Substitution ofβ-glycerophosphate for ATP produced no change in the localization of reaction product in leaf tissue. These findings are discussed in relation to current theories on the mechanism of sugar transport and phloem loading.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01279368
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  • 4
    Electronic Resource
    Electronic Resource
    The validity of the lead precipitation technique for the localization of ATPase activity in plant cells (1980)
    Springer
    Protoplasma 104 (1980), S. 193-200 
    Details
    ISSN: 1615-6102
    Keywords: Analytical electron microscopy ; ATPase cytochemistry ; Plasma membrane ; Ricinus communis ; Triticum aestivum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The claim that osmium-containing deposits which lack lead are frequently and incorrectly interpreted as enzymatic reaction products in lead precipitation techniques for ATPase localization in plants is without foundation. Proper controls clearly demonstrate the enzymatic origin of membrane-located deposits and the presence of lead is confirmed by analytical electron microscopy.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01279767
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    Paper (German National Licenses)
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  • 5
    Electronic Resource
    Electronic Resource
    The effect of calcium antagonists and inhibitors of secretory processes on auxin-induced elongation and fine structure ofPisum sativum stem segments (1986)
    Springer
    Protoplasma 133 (1986), S. 149-159 
    Details
    ISSN: 1615-6102
    Keywords: Calcium ; Cell elongation ; Indole-3-acetic acid ; Pisum sativum ; Secretion
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The treatment of dark grown pea stem segments with chelators of divalent cations (EGTA, EDTA, CTC), various Ca2+ antagonists (LaCl3, A-23187, verapamil) and inhibitors of secretory processes (monensin, CB) reduced elongation in the presence of indole-3-acetic acid (IAA). Generally the inhibition increased with increasing concentrations of the substances. The timing of the responses can be correlated with maximum auxin-stimulated secretion of cell wall material. Examination of cell ultrastructure showed that changes in dictyosome activity could explain a reduced deposition of cell wall material and so cause inhibition of elongation. The inhibitors affected the morphology and vesiculation of the dictyosomes, and the appearance of the plasma membrane, ER and mitochondria in different ways. The most pronounced effects on ultrastructure resulted from monensin and LaCl3 treatments with the dictyosomes being most affected; large vesicles appeared in the cytoplasm. Less pronounced effects on cell structure were seen in EGTA, A-23187 and verapamil treated tissue. The effects on the dictyosomes are considered to be due to disturbances of Ca2+ and other ionic levels within the cells.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01304630
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    Paper (German National Licenses)
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  • 6
    Electronic Resource
    Electronic Resource
    Cytochemical localization of plasma membrane ATPase activity in plant cells (1991)
    Springer
    Protoplasma 165 (1991), S. 27-36 
    Details
    ISSN: 1615-6102
    Keywords: ATPase ; Cerium ; Cytochemistry ; Fixation ; Zea mays roots ; Plasma membrane
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The cytochemical localization of ATPase activity has been investigated in maize root cells using both lead and cerium-based capture methods. With both methods, staining at the plasma membrane was observed in all cells of the root, although the precipitate obtained with cerium was more uniform and granular than that with lead. Controls using no substrate or no magnesium, β-glycerophosphate to replace ATP, vanadate or boiled tissue generally showed little or no staining. However, biochemical studies on purified plasma membrane fractions showed that ATPase activity was markedly inhibited by fixation, particularly by glutaraldehyde, and also by lead and cerium ions. Non-enzymic hydrolysis of ATP by cerium was greater than that by lead. The value and limitations of these procedures for the localization of plasma membrane H+-ATPase activity are summarized in relation to previous criticisms of these methods.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01322274
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    Paper (German National Licenses)
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