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  • ATTA  (1)
  • Key Words. Cotton fiber—Cytokinin—Abscisic acid—Cotton ovule—Mutant  (1)
  • Keywords Apoptosis  (1)
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  • 1
    Electronic Resource
    Electronic Resource
    Induction of apoptosis by high proinsulin and glucose in cultured human umbilical vein endothelial cells is mediated by reactive oxygen species (1998)
    Springer
    Diabetologia 41 (1998), S. 249-256 
    Details
    ISSN: 1432-0428
    Keywords: Keywords Apoptosis ; proinsulin ; umbilical vein ; endothelial cells
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary There is much evidence that diabetes and hyperglycaemia contribute to the impairment of endothelial function and induce severe changes in the proliferation, the adhesive and synthetic properties of endothelial cells. Induction of apoptosis could represent one mechanism to prevent the new accumulation of those vascular defects and to allow generation of vascular endothelium. In this study, we demonstrate that high concentrations of glucose or proinsulin induce apoptosis in human umbilical endothelial cells by three independent methods (DNA fragmentation, fluorescence activated cell sorting analysis, and morphology). The number of apoptotic cells was increased by glucose (30 mmol/l or proinsulin (100 nmol/l) from less than 10 % to about 30 %. Activation of protein kinase C (PKC) largely prevented the induction of apoptosis, whereas inhibition of PKC further increased the number of apoptotic cells. Similar changes as induced by glucose were also observed after incubation of the cells with the non-metabolisable 3-0-methylglucose. These findings indicate that hyperglycaemic conditions stimulate the induction of apoptosis in endothelial cells by a mechanism which is independent from the formation of diacylglycerol and the activation of PKC. The induction of apoptosis by the non-metabolisable glucose suggests that formation of oxygen derived radicals by autoxidative processes is involved and may lead to an activation of transcription factors such as nuclear transcription factor-ϰB (NF-ϰB) transferring the activation signal into the nucleus and leading to changes in gene expression necessary for induction of apoptosis. [Diabetologia (1998) 41: 249–256]
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001250050900
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Levels of Cytokinins in the Ovules of Cotton Mutants with Altered Fiber Development (1997)
    Springer
    Journal of plant growth regulation 16 (1997), S. 181-185 
    Details
    ISSN: 1435-8107
    Keywords: Key Words. Cotton fiber—Cytokinin—Abscisic acid—Cotton ovule—Mutant
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract. Endogenous levels of cytokinin and abscisic acid (ABA) were determined in ovules of normal cotton (TM-1) and four fiber differentiation mutants (n2, Ligon lintless, H10, and Xu142) before and after flowering by enzyme-linked immunosorbent assays. The fluctuation patterns of ABA levels in ovules of normal cotton and mutants were similar. At the fiber elongation stage, ABA content was low, and from 1 day after flowering, the ABA content decreased steadily. On the other hand, the peaks of isopentenyladenine and isopentenyladenosine in ovules of TM-1 were observed 1 day before flowering. The level of cytokinins decreased after flowering in TM-1, whereas in the mutants it increased steadily. These results indicate that endogenous ABA is probably not the main inhibitor for fiber elongation and that endogenous cytokinins likely play a dual role in fiber development. Before flowering, cytokinins function as one of the stimuli for the initiation of fibers, but after flowering, cytokinins inhibit fiber growth.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/PL00006994
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    Paper (German National Licenses)
    Fulltext
  • 3
    Electronic Resource
    Electronic Resource
    Atom trap trace analysis (2000)
    Springer
    Hyperfine interactions 127 (2000), S. 515-518 
    Details
    ISSN: 1572-9540
    Keywords: ATTA ; atom trap trace analysis ; magneto optical trap ; 85Kr ; 81Kr
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract A new method of ultrasensitive isotope trace analysis has been developed. This method, based on the technique of laser manipulation of neutral atoms, has been used to count individual 85Kr and 81Kr atoms present in a natural krypton gas sample with isotopic abundances in the range of 10−11 and 10−13, respectively. The method is free of contamination from other isotopes and elements, and can be applied to many different isotope tracers for a wide range of applications.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1012698508113
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    Paper (German National Licenses)
    Fulltext
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