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  • 1
    Electronic Resource
    Electronic Resource
    Partial amino-acid sequence of ECP31, a carrot embryogenic-cell protein, and enhancement of its accumulation by abscisic acid in somatic embryos (1992)
    Springer
    Planta 186 (1992), S. 337-342 
    Details
    ISSN: 1432-2048
    Keywords: Abscisic acid and embryogenesis ; Cell culture (embryogenesis) ; Daucus (somatic embryogenesis) ; Embryogenesis (somatic) ; LEA protein (late embryogenesis-abundant protein)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract ECP31, an embryogenic-cell protein from carrot (Daucus carota L.), was purified by sequential column-chromatographic steps and digested by V8 protease on a nitrocellulose membrane. The resultant peptides were separated by reverse-phased column chromatography and sequenced. The sequences obtained were 70–80% homologous to those of a late-embryogenesis-abundant protein (D34) from cotton (Baker et al, 1988, Plant Mol. Biol. 11, 227–291). The level of ECP31 in somatic embryos of carrot was increased by treatment of the embryos with 3.7 · 10−6 M abscisic acid (ABA) for 48 h, and there was no change in this enhanced level for up to 192 h in the presence of ABA. No similar enhancing effect of ABA was observed on the level of ECP31 in embryogenic callus or segments of carrot hypocotyls. In an immunohistochemical analysis, ECP31 was found in epidermal tissue and in the vascular system of ABA-treated somatic embryos.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00195313
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Effects of light and growth regulators on adventitious bud formation in horseradish (Armoracia rusticana) (1995)
    Springer
    Plant cell reports 14 (1995), S. 611-615 
    Details
    ISSN: 1432-203X
    Keywords: Adventitious bud formation ; Horseradish (Armoracia rusticana) ; Leaf ; Light ; Phytohormone ; Root
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary To clarify that the presence of Ri T-DNA genes are not prerequisite for the light-induced bud formation in horseradish (Armoracia rusticana) hairy roots, leaf and root segments of nontransformed horseradish plants were used as explants. Bud formation from nontransformed tissues was observed in hormone-free medium under 16 h daylight conditions, but not under continuous darkness. To investigate the effects of growth regulators on bud formation, leaf and root explants were treated with auxin (1-naphthaleneacetic acid; NAA) and / or cytokinin (6-benzyl-aminopurine; BA). The most effective treatment in the dark to stimulate bud formation was BA at 1 mg·1-1. These results show that adventitious bud formation in horseradish can be induced by light and growth regulators, and especially cytokinin, may be involved in bud formation, irrespective of whether the tissues were transformed with Ri T-DNA.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00232723
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Promotion of flowering and morphological alterations in Atropa belladonna transformed with a CaMV 35S-rolC chimeric gene of the Ri plasmid (1992)
    Springer
    Plant cell reports 12 (1992), S. 1-6 
    Details
    ISSN: 1432-203X
    Keywords: Atropa belladonna ; Flowering ; Ri plasmid ; rolC gene ; Transformation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Kanamycin-resistant plants of belladonna (Atropa belladonna) were obtained after Agrobacterium mediated transformation. When a rolC gene, which is one of the loci located on Ri plasmid of Agrobacterium rhizogenes, was co-introduced with a kanamycin resistant (NPT II) gene under control of a cauliflower mosaic virus 35S promoter, the rolC gene was expressed strongly in leaves, flowers, stems and roots. The transformed plants exhibited dramatic promotion of flowering, reduced apical dominance, pale and lanceolated leaves and smaller flowers. On the other hand, when native rolC gene was co-introduced with NPT II, the transgenic plants obtained did not exhibit the altered phenotypes observed in 35S-rolC transformants, and the expression level of the rolC gene was much lower than in 35S-rolC transformants. These results suggest that the morphological changes in transgenic Atropa belladonna were related to the degree of expression of the rolC gene.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00232412
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    Paper (German National Licenses)
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  • 4
    Electronic Resource
    Electronic Resource
    Action spectrum for light-induced formation of adventitious shoots in hairy roots of horseradish (1993)
    Springer
    Planta 189 (1993), S. 590-592 
    Details
    ISSN: 1432-2048
    Keywords: Action spectrum ; Adventitious bud formation ; Armoracia ; Hairy roots ; Light and bud formation ; Phytochrome
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract An action spectrum was determined for lightinduced formation of adventitious shoots in hairy roots of horseradish (Armoracia rusticana Gaert., Mey. et Scherb.) cultured in vitro. Near ultraviolet (350–400 nm), blue (440–460 nm) and red light (600–680 nm) were most effective in inducing formation of adventitious buds. Farred light (730 nm) inhibited the promotive effect of all three wavelength regions. These results are consistent with induction by phytochrome(s) of adventitious shoots in hairy roots of horseradish.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00198224
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    Paper (German National Licenses)
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