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  • 1
    Electronic Resource
    Electronic Resource
    Late embryogenesis abundant protein in Arabidopsis thaliana homologous to carrot ECP31 (1996)
    Oxford, UK : Blackwell Publishing Ltd
    Physiologia plantarum 98 (1996), S. 0 
    Details
    ISSN: 1399-3054
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Using carrot ECP31 cDNA as a probe, we isolated AtECP31 cDNA from a seed library of Arabidopsis thaliana. The AtECP31 cDNA contained an open reading frame of 256 amino acids. Its deduced amino acid sequence showed more than 60% identity with those of DCECP31, ZMRAB28 and GHLEA D-34 which are late embryogenesis abundant (LEA) proteins of carrot, maize and cotton, respectively. Northern blot analysis showed that the transcripts of AtECP31 accumulated specifically in mature seeds. The results suggest that AtECP31 cDNA, which encodes a LEA protein in group 5, might be involved in maturation and desiccation tolerance of seeds. Restriction fragment length polymorphism (RFLP) mapping showed that AtECP31 gene was present on chromosome 3. Cleaved amplified polymorphic sequence (CAPS) mapping allowed us to map more accurately the AtECP31 gene in the region of 28.3–29.6 cM of chromosome 3.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1399-3054.1996.tb05724.x
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  • 2
    Electronic Resource
    Electronic Resource
    4-Hydroxybenzyl alcohol accumulates in suspension-cell cultures and inhibits somatic embryogenesis in carrot (2001)
    Copenhagen : Munksgaard International Publishers
    Physiologia plantarum 112 (2001), S. 0 
    Details
    ISSN: 1399-3054
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Somatic embryogenesis in carrot (Daucus carota L.) is strongly inhibited by certain factors that accumulate in culture medium of high-density cultures of embryogenic cells. We previously identified 4-hydroxybenzyl alcohol (4HBA) as one of the inhibitory factors. In this study, we analyzed the accumulation pattern of 4HBA in the cultures of carrot suspension cells. When somatic embryogenesis was induced by culturing embryogenic cells in phytohormone-free Murashige and Skoog medium at various initial cell densities, 4HBA accumulated in the culture medium. The concentration of 4HBA in high cell density cultures was higher than in low cell density cultures. The accumulation of 4HBA in high cell density cultures was rapid during the early days of culture. This rapid accumulation of 4HBA in high cell density cultures might result in the strong inhibition of somatic embryogenesis. The production of 4HBA decreased as the somatic embryos developed. In addition, embryogenic cells released larger amount of 4HBA into the culture medium compared with non-embryogenic cells. These results suggest that the production of 4HBA is both related to embryogenic competence and developmentally regulated during somatic embryogenesis.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1034/j.1399-3054.2001.1120217.x
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  • 3
    Electronic Resource
    Electronic Resource
    Promotion of flowering and morphological alterations in Atropa belladonna transformed with a CaMV 35S-rolC chimeric gene of the Ri plasmid (1992)
    Springer
    Plant cell reports 12 (1992), S. 1-6 
    Details
    ISSN: 1432-203X
    Keywords: Atropa belladonna ; Flowering ; Ri plasmid ; rolC gene ; Transformation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Kanamycin-resistant plants of belladonna (Atropa belladonna) were obtained after Agrobacterium mediated transformation. When a rolC gene, which is one of the loci located on Ri plasmid of Agrobacterium rhizogenes, was co-introduced with a kanamycin resistant (NPT II) gene under control of a cauliflower mosaic virus 35S promoter, the rolC gene was expressed strongly in leaves, flowers, stems and roots. The transformed plants exhibited dramatic promotion of flowering, reduced apical dominance, pale and lanceolated leaves and smaller flowers. On the other hand, when native rolC gene was co-introduced with NPT II, the transgenic plants obtained did not exhibit the altered phenotypes observed in 35S-rolC transformants, and the expression level of the rolC gene was much lower than in 35S-rolC transformants. These results suggest that the morphological changes in transgenic Atropa belladonna were related to the degree of expression of the rolC gene.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00232412
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  • 4
    Electronic Resource
    Electronic Resource
    Alkaloid production by hairy root cultures in Atropa belladonna (1986)
    Springer
    Plant cell reports 5 (1986), S. 239-242 
    Details
    ISSN: 1432-203X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Hairy roots were induced by inoculation of stems of sterile plants of Atropa belladonna with Agrobacterium rhizogenes. The axenic culture of the hairy roots isolated from the stems proliferated 60 fold as based on the initial fresh weight after one month of culture. The presence of atropine and scopolamine in hairy roots were examined by TLC and HPLC. Their amounts were analyzed by GLC. The results show that the amount of the two alkaloids in the axenic cultures was the same as or even higher than those of normal plants grown in the field.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00269811
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  • 5
    Electronic Resource
    Electronic Resource
    Partial amino-acid sequence of ECP31, a carrot embryogenic-cell protein, and enhancement of its accumulation by abscisic acid in somatic embryos (1992)
    Springer
    Planta 186 (1992), S. 337-342 
    Details
    ISSN: 1432-2048
    Keywords: Abscisic acid and embryogenesis ; Cell culture (embryogenesis) ; Daucus (somatic embryogenesis) ; Embryogenesis (somatic) ; LEA protein (late embryogenesis-abundant protein)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract ECP31, an embryogenic-cell protein from carrot (Daucus carota L.), was purified by sequential column-chromatographic steps and digested by V8 protease on a nitrocellulose membrane. The resultant peptides were separated by reverse-phased column chromatography and sequenced. The sequences obtained were 70–80% homologous to those of a late-embryogenesis-abundant protein (D34) from cotton (Baker et al, 1988, Plant Mol. Biol. 11, 227–291). The level of ECP31 in somatic embryos of carrot was increased by treatment of the embryos with 3.7 · 10−6 M abscisic acid (ABA) for 48 h, and there was no change in this enhanced level for up to 192 h in the presence of ABA. No similar enhancing effect of ABA was observed on the level of ECP31 in embryogenic callus or segments of carrot hypocotyls. In an immunohistochemical analysis, ECP31 was found in epidermal tissue and in the vascular system of ABA-treated somatic embryos.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00195313
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  • 6
    Electronic Resource
    Electronic Resource
    Action spectrum for light-induced formation of adventitious shoots in hairy roots of horseradish (1993)
    Springer
    Planta 189 (1993), S. 590-592 
    Details
    ISSN: 1432-2048
    Keywords: Action spectrum ; Adventitious bud formation ; Armoracia ; Hairy roots ; Light and bud formation ; Phytochrome
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract An action spectrum was determined for lightinduced formation of adventitious shoots in hairy roots of horseradish (Armoracia rusticana Gaert., Mey. et Scherb.) cultured in vitro. Near ultraviolet (350–400 nm), blue (440–460 nm) and red light (600–680 nm) were most effective in inducing formation of adventitious buds. Farred light (730 nm) inhibited the promotive effect of all three wavelength regions. These results are consistent with induction by phytochrome(s) of adventitious shoots in hairy roots of horseradish.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00198224
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  • 7
    Electronic Resource
    Electronic Resource
    Effects of light and growth regulators on adventitious bud formation in horseradish (Armoracia rusticana) (1995)
    Springer
    Plant cell reports 14 (1995), S. 611-615 
    Details
    ISSN: 1432-203X
    Keywords: Adventitious bud formation ; Horseradish (Armoracia rusticana) ; Leaf ; Light ; Phytohormone ; Root
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary To clarify that the presence of Ri T-DNA genes are not prerequisite for the light-induced bud formation in horseradish (Armoracia rusticana) hairy roots, leaf and root segments of nontransformed horseradish plants were used as explants. Bud formation from nontransformed tissues was observed in hormone-free medium under 16 h daylight conditions, but not under continuous darkness. To investigate the effects of growth regulators on bud formation, leaf and root explants were treated with auxin (1-naphthaleneacetic acid; NAA) and / or cytokinin (6-benzyl-aminopurine; BA). The most effective treatment in the dark to stimulate bud formation was BA at 1 mg·1-1. These results show that adventitious bud formation in horseradish can be induced by light and growth regulators, and especially cytokinin, may be involved in bud formation, irrespective of whether the tissues were transformed with Ri T-DNA.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00232723
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  • 8
    Electronic Resource
    Electronic Resource
    Shikonin production and secretion by hairy root cultures of Lithospermum erythrorhizon (1991)
    Springer
    Plant cell reports 10 (1991), S. 282-285 
    Details
    ISSN: 1432-203X
    Keywords: Lithospermum erythrorhizon ; Hairy root culture ; Shikonin ; Secretion ; Adsorbent
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Hairy root cultures of Lithospermum erythrorhizon were established by transformation of in vitro grown shoots with Agrobacterium rhizogenes 15834. Hairy roots cultured on Murashige and Skoog solid medium did not produce any red pigments. However, the hairy roots cultured in Root Culture solid or liquid media produced a large amount of red pigments, which were released to the medium. The addition of adsorbents to the culture medium stimulated shikonin production by ca. 3-fold. Using this method an air-lift fermenter system was established, equipped with a XAD-2 column, which continuously produced ca. 5 mg/day of shikonin during a period of more than 220 days.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00193142
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  • 9
    Electronic Resource
    Electronic Resource
    Genetic transformation of Begonia tuberhybrida by Ri rol genes (1996)
    Springer
    Plant cell reports 15 (1996), S. 606-609 
    Details
    ISSN: 1432-203X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract We have developed an Agrobacterium -mediated transformation system for commercial Begonia species. The leaf explants of Begonia semperflorens, Begonia x hiemalis and B. tuberhybrida were inoculated with Agrobacterium tumefaciens LBA4404 harboring a binary vector pBI121 which contains rolA, B and C genes of an agropine type Ri plasmid (pRiA4b). Kanamycin resistant shoots of B. tuberhybrida were obtained on MS agar medium supplemented with 0.1 mg/l NAA, 0.5 mg/l BA, 500 mg/l claforan and 100 mg/l kanamycin. These shoots exhibited GUS activity and Southern analysis showed a single copy insertion into the genome. When the transgenic plants were transferred to soil, they displayed the phenotype specific to the transgenic plants by A. rhizogenes such as dwarfness, delay of flowering, and wrinkled leaves and petals.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00232462
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  • 10
    Electronic Resource
    Electronic Resource
    Genetic transformation of Begonia tuberhybrida by Ri rol genes (1996)
    Springer
    Plant cell reports 15 (1996), S. 606-609 
    Details
    ISSN: 1432-203X
    Keywords: Abbreviations: NAA: 1-naphthaleneacetic acid; BA: benzyladenine; MS: Murashige and Skoog (1962); GUS: β-glucuronidase (EC 3.2.1.31); NPT II: neomycin phosphotransferase (EC 2.7.1.95); NOS: nopaline synthase; CaMV: Cauliflower Mosaic Virus; CTAB: cetyl triethylammonium bromide; X-GLUC: 5-bromo-4-chloro-3-indolyl-β-D-glucuronide; PCR: polymerase chain reaction
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract. We have developed an Agrobacterium-mediated transformation system for commercial Begonia species. The leaf explants of Begonia semperflorens, Begonia x hiemalis and B. tuberhybrida were inoculated with Agrobacterium tumefaciens LBA4404 harboring a binary vector pBI121 which contains rolA,B and C genes of an agropine type Ri plasmid (pRiA4b). Kanamycin resistant shoots of B. tuberhybrida were obtained on MS agar medium supplemented with 0.1 mg/l NAA, 0.5 mg/l BA, 500 mg/l claforan and 100 mg/l kanamycin. These shoots exhibited GUS activity and Southern analysis showed a single copy insertion into the genome. When the transgenic plants were transferred to soil, they displayed the phenotype specific to the transgenic plants by A. rhizogenes such as dwarfness, delay of flowering, and wrinkled leaves and petals.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002990050083
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