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  • Achalasia  (1)
  • Key words: Bone-specific alkaline phosphatase — Purification — Saos-2 cell — Serial lectin affinity chromatography.  (1)
  • Osteopenia  (1)
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Material
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Keywords
  • 1
    Electronic Resource
    Electronic Resource
    Circadian rhythm in type I collagen formation in postmenopausal women with and without osteopenia (1995)
    Springer
    Osteoporosis international 5 (1995), S. 472-477 
    Details
    ISSN: 1433-2965
    Keywords: Circadian rhythm ; Cosinor analysis ; Osteopenia ; Postmenopause ; Procollagen type I carboxyl-terminal propeptide (PICP)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract A circadian rhythm in the serum concentration of the procollagen type I carboxyl-terminal propeptide (sPICP) has previously been demonstrated in premenopausal women. This study was performed to investigate the circadian rhythm in sPICP in healthy and osteopenic postmenopausal women. Blood samples were taken every third hour for 27 h from three groups of women: 12 early postmenopausal women (aged 55±2 years; mean±SD); 12 late postmenopausal women (aged 73±1 years); and 12 osteopenic but otherwise healthy late postmenopausal women (aged 73±1 years). A circadian rhythm in sPICP was found in all three groups, as shown by cosinor analysis (p=0.000003−0.03). The circadian rhythm in sPICP was significantly different between the osteopenic group and the age-matched healthy group (p〈0.008). The amplitude of the circadian rhythm in sPICP was about twice as high in the osteopenic group, and the time of the maximum tended to be about 3 h later, as compared with the age-matched healthy group. The plasma concentration of osteocalcin, as measured by a recently developed two-site enzyme-linked immunosorbent assay, also showed a circadian rhythm in all three groups (p=0.0001−0.05), with no significant differences between groups. In conclusion, we have found a significant circadian rhythm in sPICP in both early and late postmenopausal women. In osteopenic women the nightly peak in sPICP is larger and persists later into the night as compared with non-osteopenic women.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01626611
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Radiographic evaluation of acute tracheal obstruction in chalasia (1987)
    Springer
    Dysphagia 1 (1987), S. 217-220 
    Details
    ISSN: 1432-0460
    Keywords: Achalasia ; Airway obstruction ; Esophagus
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract This report describes the clinical and radiographic features of three patients with achalasia who developed acute upper airway obstruction secondary to dilatation of the esophagus. Computed tomography was especially useful in confirming the diagnoses in two cases. Standard radiographs of the cervical area demonstrated obstruction of the larynx in the third case. In all cases, relief of respiratory distress was achieved by decompression of the esophagus.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02406921
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Purification and Characterization of Bone-Specific Alkaline Phosphatase from a Human Osteosarcoma Cell Line (1998)
    Springer
    Calcified tissue international 62 (1998), S. 67-73 
    Details
    ISSN: 1432-0827
    Keywords: Key words: Bone-specific alkaline phosphatase — Purification — Saos-2 cell — Serial lectin affinity chromatography.
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Physics
    Notes: Abstract. Bone-specific alkaline phosphatase (bone ALP) levels are considered to reflect osteoblastic activity and can therefore be used as a marker of bone formation. However, bone ALP is difficult to distinguish from other ALP isoforms since the kidney, liver, and bone isoenzymes are encoded by the same gene and only differ because of post-translational modification of their carbohydrate side chains. The aim of this study was to purify and separate bone ALP which could be used to raise specific antisera against human bone ALP, from Saos-2, a human osteogenic sarcoma cell line. The procedure involved two steps. The first step, cultivation of 105 Saos-2 cells, yielded approximately 1 U ALP. Subsequent butanol extraction achieved 1.82-fold purification. For the second step, separating bone ALP, we used serial lectin affinity chromatography to distinguish between the carbohydrate side chains of the various ALP isoforms. A sample of the butanol extract was fractionated into three peaks (I, II, and III) by concanavalin A. Peaks II and III were subsequently identified as types IIa and IIIb bone ALP using pea lectin and wheat germ agglutinin columns, respectively. The specific activity of bone ALP was measured using commercial kits. Since bone ALP accounted for at least 84% of the total ALP activity after the final separation, this method appears more convenient and reproducible than others using bone or Pagetic sera. The bone ALP purified in this study could be used to raise monoclonal antibodies against bone-specific ALP.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002239900396
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    Paper (German National Licenses)
    Fulltext
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