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  • 1
    Electronic Resource
    Electronic Resource
    Circadian rhythm in type I collagen formation in postmenopausal women with and without osteopenia (1995)
    Springer
    Osteoporosis international 5 (1995), S. 472-477 
    Details
    ISSN: 1433-2965
    Keywords: Circadian rhythm ; Cosinor analysis ; Osteopenia ; Postmenopause ; Procollagen type I carboxyl-terminal propeptide (PICP)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract A circadian rhythm in the serum concentration of the procollagen type I carboxyl-terminal propeptide (sPICP) has previously been demonstrated in premenopausal women. This study was performed to investigate the circadian rhythm in sPICP in healthy and osteopenic postmenopausal women. Blood samples were taken every third hour for 27 h from three groups of women: 12 early postmenopausal women (aged 55±2 years; mean±SD); 12 late postmenopausal women (aged 73±1 years); and 12 osteopenic but otherwise healthy late postmenopausal women (aged 73±1 years). A circadian rhythm in sPICP was found in all three groups, as shown by cosinor analysis (p=0.000003−0.03). The circadian rhythm in sPICP was significantly different between the osteopenic group and the age-matched healthy group (p〈0.008). The amplitude of the circadian rhythm in sPICP was about twice as high in the osteopenic group, and the time of the maximum tended to be about 3 h later, as compared with the age-matched healthy group. The plasma concentration of osteocalcin, as measured by a recently developed two-site enzyme-linked immunosorbent assay, also showed a circadian rhythm in all three groups (p=0.0001−0.05), with no significant differences between groups. In conclusion, we have found a significant circadian rhythm in sPICP in both early and late postmenopausal women. In osteopenic women the nightly peak in sPICP is larger and persists later into the night as compared with non-osteopenic women.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01626611
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  • 2
    Electronic Resource
    Electronic Resource
    Purification and Characterization of Bone-Specific Alkaline Phosphatase from a Human Osteosarcoma Cell Line (1998)
    Springer
    Calcified tissue international 62 (1998), S. 67-73 
    Details
    ISSN: 1432-0827
    Keywords: Key words: Bone-specific alkaline phosphatase — Purification — Saos-2 cell — Serial lectin affinity chromatography.
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Physics
    Notes: Abstract. Bone-specific alkaline phosphatase (bone ALP) levels are considered to reflect osteoblastic activity and can therefore be used as a marker of bone formation. However, bone ALP is difficult to distinguish from other ALP isoforms since the kidney, liver, and bone isoenzymes are encoded by the same gene and only differ because of post-translational modification of their carbohydrate side chains. The aim of this study was to purify and separate bone ALP which could be used to raise specific antisera against human bone ALP, from Saos-2, a human osteogenic sarcoma cell line. The procedure involved two steps. The first step, cultivation of 105 Saos-2 cells, yielded approximately 1 U ALP. Subsequent butanol extraction achieved 1.82-fold purification. For the second step, separating bone ALP, we used serial lectin affinity chromatography to distinguish between the carbohydrate side chains of the various ALP isoforms. A sample of the butanol extract was fractionated into three peaks (I, II, and III) by concanavalin A. Peaks II and III were subsequently identified as types IIa and IIIb bone ALP using pea lectin and wheat germ agglutinin columns, respectively. The specific activity of bone ALP was measured using commercial kits. Since bone ALP accounted for at least 84% of the total ALP activity after the final separation, this method appears more convenient and reproducible than others using bone or Pagetic sera. The bone ALP purified in this study could be used to raise monoclonal antibodies against bone-specific ALP.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002239900396
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  • 3
    Electronic Resource
    Electronic Resource
    Radiographic evaluation of acute tracheal obstruction in chalasia (1987)
    Springer
    Dysphagia 1 (1987), S. 217-220 
    Details
    ISSN: 1432-0460
    Keywords: Achalasia ; Airway obstruction ; Esophagus
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract This report describes the clinical and radiographic features of three patients with achalasia who developed acute upper airway obstruction secondary to dilatation of the esophagus. Computed tomography was especially useful in confirming the diagnoses in two cases. Standard radiographs of the cervical area demonstrated obstruction of the larynx in the third case. In all cases, relief of respiratory distress was achieved by decompression of the esophagus.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02406921
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    Paper (German National Licenses)
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  • 4
    Electronic Resource
    Electronic Resource
    A comparative study of intravenous cholangiography and99mTc-pyridoxylideneglutamate in patients with hepatobiliary disease (1977)
    Springer
    Abdominal imaging 2 (1977), S. 141-144 
    Details
    ISSN: 1432-0509
    Keywords: Biliary scanning ; Intravenous cholangiography ; Jaundice ; Biliary tract disease ; 99mTc-Pyridoxylideneglutamate
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract The purpose of this investigation was to compare the diagnostic value of intravenous cholangiography with99mTc-pyridoxylideneglutamate, a new radioisotopic biliary imaging agent, in 15 patients with suspected hepatobiliary disease. Each subject had both examinations, and the studies were interpreted independently. The results suggest that intravenous cholangiography may provide more specific information about the ductal system but is of limited value in patients with elevated bilirubin. In these patients99mTc-pyridoxylideneglutamate may show patency of the bile ducts and distinguish between partial and complete biliary tract obstruction. Depending upon the serum bilirubin level, both studies may provide useful and complementary information about the biliary tract.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02256487
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    Paper (German National Licenses)
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  • 5
    Electronic Resource
    Electronic Resource
    Queueing analysis: A useful planning and management technique for radiology (1987)
    Springer
    Journal of medical systems 11 (1987), S. 413-419 
    Details
    ISSN: 1573-689X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Queueing analysis is a mathematical technique which is used for studying characteristics of queueing (waiting) for service. Recent development of imporved software systems has made the technique easily available and versatile using a microcomputer. In this study the characteristics of examination time and interarrival time were determined for an emergency room radiology service, and two examples were analyzed that demonstrate the usefulness of queueing analysis in radiology. In the first example the effects of a 5% annual increase in patients on waiting time are shown, while the second example shows the use of the technique for cost analysis. Radiologists and medical administrators should be aware of the availability and value of queueing analysis for department planning and management.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00993008
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    Paper (German National Licenses)
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  • 6
    Electronic Resource
    Electronic Resource
    Enzyme-linked immunosorbent assay of urokinase-type plasminogen activator (uPA) in cytosolic extracts of human breast cancer tissue (1993)
    Springer
    Breast cancer research and treatment 28 (1993), S. 223-229 
    Details
    ISSN: 1573-7217
    Keywords: breast cancer ; cytosols ; ELISA ; urokinase-type plasminogen activator
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The enzyme urokinase-type plasminogen activator (uPA) plays a role in cancer invasion, and high levels of uPA in detergent extracts of mammary cancer tissue have been reported to be associated with a poor prognosis. We have explored the possibility of using mammary cancer cytosol extracts routinely prepared for steroid receptor analysis for retrospective prognostic studies of uPA. A sandwich enzyme-linked immunosorbent assay (ELISA) for uPA was developed, using polyclonal catching antibodies and a mixture of three biotinylated monoclonal detecting antibodies, that were selected to recognize free uPA, inhibitor-bound uPA, and uPA bound to its cell surface receptor. The assay detects active uPA and its inactive proenzyme form, pro-uPA, equally well. The limit of detection is approximately 1 pg of pro-uPA in a volume of 100 µl, and there is a linear dose-response up to 100 pg pro-uPA. The efficiency in extracting uPA of a neutral non-detergent buffer used to prepare cytosol extracts was compared with that of 4 other buffers. There was a pronounced difference in the efficiency, the most efficient being a pH 4.2 buffer containing the non-ionic detergent Triton X-100, while the least efficient was the buffer used to prepare cytosols. Nevertheless, uPA immunoreactivity was readily measurable in the cytosols, and there was a close correlation between the amounts of uPA extracted under optimal conditions and those routinely used for steroid hormone receptor analysis. While the amount of uPA extracted under the latter conditions constituted only about 12% of that optimally extractable, we conclude that the ELISA described herein can be used to retrospectively analyze the potential prognostic value of uPA-concentrations in cytosols prepared for analysis of steroid hormone receptors.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00666583
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