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  • 1
    Electronic Resource
    Electronic Resource
    Persistence of B19 parvovirus in synovial membranes of patients with rheumatoid arthritis (1992)
    Springer
    Rheumatology international 12 (1992), S. 147-151 
    Details
    ISSN: 1437-160X
    Keywords: Rheumatoid arthritis ; B19 parvovirus ; Polymerase chain reaction
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Recent clinical observations support the hypothesis that persistent parvovirus B19 is a triggering factor of rheumatoid arthritis (RA) in certain genetically predisposed individuals. If this hypothesis is correct, a number of RA patients may exhibit parvovirus B19 DNA in their synovial membranes. We tested the synovial tissue and peripheral blood leukocytes of 20 patients with RA, 24 patients with other arthritides or osteoarthritis (non-RA), and 34 healthy blood donors for the presence of parvovirus B19 DNA using specific DNA amplification by polymerase chain reaction (PCR). Using this technique, parvovirus B19 DNA was demonstrated in the synovial biopsies of 75% of patients with RA but in those of only 16.7% of patients with non-RA. In autologous peripheral blood mononuclear cells the percentage of PCR-positive patients was about 15% in both RA and non-RA groups and did not differ from that in healthy controls. When the PCR data were correlated with the presence of anti-parvovirus B19 IgG antibodies in serum and synovia all patients with parvovirus B19 DNA in peripheral blood alone or in both peripheral blood and synovial membrane were seropositive. In contrast, about 40% of patients with parvovirus B19 DNA restricted to the synovial membrane were seronegative. These data indicate a highly disease-related persistence of parvovirus B19 in the rheumatoid synovium.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00274934
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  • 2
    Electronic Resource
    Electronic Resource
    Isoenzym-Muster saurer Phosphatasen aus normalen und leukämischen weißen Blutzellen (1977)
    Springer
    Annals of hematology 35 (1977), S. 295-303 
    Details
    ISSN: 1432-0584
    Keywords: Acid phosphatases ; Isoenzyme pattern ; Leukemic blood cells ; Hairy-cell-leukemia ; Saure Phosphatasen ; Isoenzym-Muster ; Leukämische Blutzellen ; Haarzell-Leukämie
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Zusammenfassung Die durchschnittlichen Aktivitäten (IE/106 Zellen) der sauren Phosphatasen und die prozentuale Verteilung der Isoenzyme der sauren Phosphatasen wurden aus Lysaten von normalen und leukämischen weißen Blutzellen bestimmt. Bei 4 Patienten mit Haarzell-Leukämie lag der Anteil des Isoenzyms 5 geringfügig über dem der tartratresistenten Zellen. Bei 2 Patienten mit einer fraglichen Haarzell-Leukämie waren deutliche Isoenzym-5-Fraktionen, aber keine tartratresistenten Zellen zu erkennen. Bei einem Patienten mit Haarzell-Leukämie wurde bei einem hohem Anteil tartratresistenter Haarzellen eine auffallend geringere Isoenzym-5-Fraktion gefunden. Diese unterschiedlichen Beziehungen wurden mit der empfindlichen Nachweismethode der Gelelektrophorese und unterschiedlicher Substrataffinität erklärt.
    Notes: Summary The activities of acid phosphatases (AP) were measured in leukocytes from patients with chronic myelocytic leukemia (CML), macrophages, granulocytes, in the fractionated mononuclear cells of patients with CML and with hairy-cell-leukemia (HCL) and in the cells from patients with acute leukemia (AL). The lowest activities were found in lymphocytes of normal subjects and of patients with chronic lymphatic leukemia (CLL) and in thrombocytes. Isoenzyme (IsE) l was characteristic for thymocytes, IsE 2 for granulocytes, IsE 3 for pathologic blast-cells, lymphocytes and thrombocytes, IsE 4 for macrophages, IsE 5 with components a and b for the mononuclear fraction of patients with HCL. In addition IsE5 was detected in lymphocytes, macrophages and CLL-cells. In 4 patients with HCL the relative percentage of IsE-5-fraction was slightly greater than the percentage of tartrate resistant cells. In two patients with questionable HCL well marked IsE-5-fractions were recognized but no tartrate resistant cells. In one patient with HCL a relatively high percentage of tartrate resistant hairy-cells and in comparison an inadäquate low IsE-5-fraction was found. These different relations were explained with the more sensitive method of gelelectrophoresis and different affinity of substrates to AP.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00996142
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    Paper (German National Licenses)
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