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  • 1
    ISSN: 1432-0878
    Keywords: Thymus ; Myosin ; Actin ; Immunofluorescence microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary A sensitive immunofluorescence microscopical technique employing specific antibodies against highly purified actin and (smooth muscle type) myosin from chicken gizzard and myosin from human striated muscle was used to localize these contractile proteins in the thymus of rat, guinea-pig, cat and chicken. Myoid cells were seen to react with antibodies to striated, but not to smooth muscle type myosin, whereas reticular epithelial cells contained smooth, but not striated type myosin. Actin immunoreactivity was found in both myoid and reticular epithelial cells.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1573-8469
    Keywords: Fusarium head blight ; mycotoxins ; plant breeding ; resistance
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Heads of 12 spring barley genotypes (eight cultivars, four lines) were inoculated with conidial suspensions of twoFusarium culmorum isolates (I1 and I2). Both isolates caused the following significant reductions (%) when compared with the control: number of kernels head−1 12 and 16, weight of 1000 kernels 47 and 24, yield 49 and 37, for I1 and I2 respectively. Both isolates were able to produce average levels (mg kg−1) of deoxynivalenol of 67.1 and 13.9, 3-acetyldeoxynivalenol of 9.4 and 1.4 and zearalenone of 0.3 and 0.4 for I1 and I2 respectively. In kernels of three genotypes inoculated with the same isolates, 15-acetyldeoxynivalenol at an average level (mg kg−1) of 1.3 and 1.5 was detected, while in kernels of six genotypes inoculated with I2, 0.2 of nivalenol was found. A significant correlation for yield and toxin level was found for less pathogenicF. culmorum isolate I2.
    Type of Medium: Electronic Resource
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  • 3
    ISSN: 1573-8469
    Keywords: Fusarium head blight ; mycotoxins ; plant breeding ; resistance
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Twelve barley genotypes were inoculated with two F. graminearum isolates of different chemotype I1 #148 (producing nivalenol/deoxynivalenol) and I2 #108 (deoxynivalenol/acetyldeoxynivalenol). For both I1 and I2 isolates, respectively, reductions (%) in number of kernels head-1 10.6 and 14.3; yield 39.6 and 35.7; weight of 1000 kernels 36.9 and 23.2 were observed in inoculated plants from control values. Chemical analysis revealed the presence (average concentration mg kg-1) of deoxynivalenol (1.3) and nivalenol (3.2) in kernels of all genotypes inoculated with the I1 isolate, and zearalenone (0.2) in three samples. After inoculation with the I2 isolate, deoxynivalenol (37.8) and zearalenone (0.4) were found in kernels of all genotypes, while 3-acetyldeoxynivalenol and 15-acetyldeoxynivalenol, respectively, were determined in five and four samples only. No significant correlation between examined characteristics was found for either the I1 or I2 isolate. The results obtained contribute information on the accumulation of toxins in cereal grain inoculated with F. graminearum isolates of different chemotypes.
    Type of Medium: Electronic Resource
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