Library

X
feed icon rss

Your email was sent successfully. Check your inbox.

An error occurred while sending the email. Please try again.

Proceed reservation?

Export
  • 1
    Electronic Resource
    Electronic Resource
    Locally evoked potentials in slices of rat neostriatum: A tool for the investigation of intrinsic excitatory processes (1979)
    Springer
    Experimental brain research 34 (1979), S. 575-590 
    Details
    ISSN: 1432-1106
    Keywords: Neostriatum slices ; Field potentials ; Action potentials ; EPSPs ; Intrinsic excitation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Field potentials, extracellular unitary discharges and intracellular potentials evoked by intrastriatal stimulation were recorded from slices (thickness 200–400 μm) of rat neostriatum maintained in an artificial medium. The field potentials consisted of two negative waves appearing at latencies of 0.5–1.5 ms (N-1) and 2–4 ms (N-2). Extracellular unitary records showed two types of discharges, one with short but constant latencies at threshold level stimulation and the other with longer and variable latencies. In intracellular recordings the late discharge was seen to arise from EPSPs. Based on the intra- and extracellular unitary records, N-1 was identified as the population spike of antidromically or directly activated unitary discharges and N-2 as that of orthodromically activated discharges. This interpretation was substantiated by the fact that the N-2 potential was blocked in a perfusion medium containing a lower Ca++ or a higher Mg++ concentration than the standard solution. Neither interruption of ascending neostriatal inputs nor decortication 14 days prior to recording altered the configuration of the locally evoked potentials or the probability of synaptically driven discharge occurrence. Thus by intrastriatal stimulation, neostriatal neurons are activated antidromically or directly and/or orthodromically through intrinsic excitatory synapses. Since the intracellular recordings showed that neostriatal neurons can be well preserved, this preparation can be regarded as a useful tool for electrophysiological and neuropharmacological investigations on intrinsic excitatory processes in the neostriatum.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00239150
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 2
    Electronic Resource
    Electronic Resource
    (–)-Baclofen-induced and constitutively active inwardly rectifying potassium conductances in cultured rat midbrain neurons (1996)
    Springer
    Pflügers Archiv 433 (1996), S. 49-57 
    Details
    ISSN: 1432-2013
    Keywords: Key words Inward rectification ; Baclofen ; Barium ; Caesium ; Development
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract  Biophysical and pharmacological properties, and development of the (–)-baclofen-induced potassium (KBac) conductance and the constitutively active inwardly rectifying potassium (KIR) conductance were characterised using the patch-clamp technique in cultured embryonic rat midbrain neurons. KBac conductance was induced by (–)-baclofen acting on γ-aminobutyric acid B (GABAB) receptors, and displayed a high degree of selectivity for potassium ions, an approximate square-root dependence of conductance on extracellular potassium concentration and strongly voltage-dependent activation. Ba2+ blocked the conductance in a voltage-independent manner, whereas Cs+ produced a voltage-dependent block. In the same preparation, the KIR conductance displayed biophysical properties indistinguishable from those of the KBac conductance. Block of KIR currents by Ba2+ was voltage independent (K I, 4 μM), whereas Cs+ produced a voltage-dependent block (K I, 370 μM at –100 mV, equivalent valence, z′, 1.67). The KBac and KIR conductances additionally displayed a strikingly similar pattern of development in culture; the specific conductance (nS/pF) of both conductances increased two- to three-fold between the first and second week in vitro, and remained constant thereafter.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004240050247
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
Close ⊗
This website uses cookies and the analysis tool Matomo. More information can be found here...